Pulsed Electric Field Extraction of α and β-Acids From Pellets of Humulus lupulus (Hop)

This paper investigates the process of extracting hop pellets (hops) utilizing the pulsed electric field (PEF) technique and the contrasting effects of the technique between two distinct hop varieties (one bitter and one aromatic). The effect of PEF on the extraction was evaluated by measuring the c...

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Main Authors: George Ntourtoglou, Evangelia Anastasia Tsapou, Fotini Drosou, Eleni Bozinou, Stavros Lalas, Panagiotis Tataridis, Vassilis Dourtoglou
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-04-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2020.00297/full
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author George Ntourtoglou
Evangelia Anastasia Tsapou
Fotini Drosou
Eleni Bozinou
Stavros Lalas
Panagiotis Tataridis
Vassilis Dourtoglou
author_facet George Ntourtoglou
Evangelia Anastasia Tsapou
Fotini Drosou
Eleni Bozinou
Stavros Lalas
Panagiotis Tataridis
Vassilis Dourtoglou
author_sort George Ntourtoglou
collection DOAJ
description This paper investigates the process of extracting hop pellets (hops) utilizing the pulsed electric field (PEF) technique and the contrasting effects of the technique between two distinct hop varieties (one bitter and one aromatic). The effect of PEF on the extraction was evaluated by measuring the concentration of α-acids and β-acids (humulones and lupulones). Regarding the aromatic character, the hop’s volatile caryophyllene, humulene and β-myrcene were analyzed both with and without employing the PEF treatment. In order to analyze the acids and the volatile fraction, the analytical method of UV–vis spectrophotometry was applied followed by gas chromatography coupled with mass spectrometry. For the second technique, the extracts were previously purified through a Graphitized Carbon Black syringe for Solid Phase Extraction. The results revealed that PEF had a positive impact on the alpha acids of bitter hops by increasing the extraction rate of these acids by 20%, while the volatiles demonstrated an increase of 5.6 and 7.4% for humulene and caryophyllene, respectively. Concerning the aromatic variety of hops, the PEF treatment appeared to have no noteworthy effects.
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spelling doaj.art-f91f0de9f9c54971b241e715c14d03ed2022-12-22T00:50:29ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-04-01810.3389/fbioe.2020.00297500537Pulsed Electric Field Extraction of α and β-Acids From Pellets of Humulus lupulus (Hop)George Ntourtoglou0Evangelia Anastasia Tsapou1Fotini Drosou2Eleni Bozinou3Stavros Lalas4Panagiotis Tataridis5Vassilis Dourtoglou6Department of Wine, Vine and Beverage Sciences, University of West Attica, Athens, GreeceDepartment of Wine, Vine and Beverage Sciences, University of West Attica, Athens, GreeceDepartment of Wine, Vine and Beverage Sciences, University of West Attica, Athens, GreeceDepartment of Food Science and Nutrition, University of Thessaly, Karditsa, GreeceDepartment of Food Science and Nutrition, University of Thessaly, Karditsa, GreeceDepartment of Wine, Vine and Beverage Sciences, University of West Attica, Athens, GreeceDepartment of Wine, Vine and Beverage Sciences, University of West Attica, Athens, GreeceThis paper investigates the process of extracting hop pellets (hops) utilizing the pulsed electric field (PEF) technique and the contrasting effects of the technique between two distinct hop varieties (one bitter and one aromatic). The effect of PEF on the extraction was evaluated by measuring the concentration of α-acids and β-acids (humulones and lupulones). Regarding the aromatic character, the hop’s volatile caryophyllene, humulene and β-myrcene were analyzed both with and without employing the PEF treatment. In order to analyze the acids and the volatile fraction, the analytical method of UV–vis spectrophotometry was applied followed by gas chromatography coupled with mass spectrometry. For the second technique, the extracts were previously purified through a Graphitized Carbon Black syringe for Solid Phase Extraction. The results revealed that PEF had a positive impact on the alpha acids of bitter hops by increasing the extraction rate of these acids by 20%, while the volatiles demonstrated an increase of 5.6 and 7.4% for humulene and caryophyllene, respectively. Concerning the aromatic variety of hops, the PEF treatment appeared to have no noteworthy effects.https://www.frontiersin.org/article/10.3389/fbioe.2020.00297/fullhopspulsed electric fieldα-acidsβ-acidsextractionSPE
spellingShingle George Ntourtoglou
Evangelia Anastasia Tsapou
Fotini Drosou
Eleni Bozinou
Stavros Lalas
Panagiotis Tataridis
Vassilis Dourtoglou
Pulsed Electric Field Extraction of α and β-Acids From Pellets of Humulus lupulus (Hop)
Frontiers in Bioengineering and Biotechnology
hops
pulsed electric field
α-acids
β-acids
extraction
SPE
title Pulsed Electric Field Extraction of α and β-Acids From Pellets of Humulus lupulus (Hop)
title_full Pulsed Electric Field Extraction of α and β-Acids From Pellets of Humulus lupulus (Hop)
title_fullStr Pulsed Electric Field Extraction of α and β-Acids From Pellets of Humulus lupulus (Hop)
title_full_unstemmed Pulsed Electric Field Extraction of α and β-Acids From Pellets of Humulus lupulus (Hop)
title_short Pulsed Electric Field Extraction of α and β-Acids From Pellets of Humulus lupulus (Hop)
title_sort pulsed electric field extraction of α and β acids from pellets of humulus lupulus hop
topic hops
pulsed electric field
α-acids
β-acids
extraction
SPE
url https://www.frontiersin.org/article/10.3389/fbioe.2020.00297/full
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