Morphological study of Schwann cells remyelination in contused spinal cord of rats
【Abstract】Objective: To study the role and effect of Schwann cells (SCs) remyelination in contused spinal cord. Methods: Green fluorescence protein expressing-SCs were transplanted into the epicenter, rostral and caudal tis-sues of the injury site at 1 week after the spinal cords were con...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2013-08-01
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| Series: | Chinese Journal of Traumatology |
| Online Access: | http://www.cjtrauma.com/apps/ojs/index.php/cjt/article/view/429 |
| _version_ | 1819044266393993216 |
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| author | LI Yue ZHANG Lu ZHANG Jie-yuan LIU Zheng DUAN Zhao-xia LI Bing-cang |
| author_facet | LI Yue ZHANG Lu ZHANG Jie-yuan LIU Zheng DUAN Zhao-xia LI Bing-cang |
| author_sort | LI Yue |
| collection | DOAJ |
| description | 【Abstract】Objective: To study the role and effect
of Schwann cells (SCs) remyelination in contused spinal
cord.
Methods: Green fluorescence protein expressing-SCs
were transplanted into the epicenter, rostral and caudal tis-sues of the injury site at 1 week after the spinal cords were
contused. At 6 weeks, the spinal cords were removed for
cryosections, semithin sections and ultrathin sections, and
then immunocytochemical staining of myelin basic protein
(MBP), P0 protein (P0) and S100 protein (S100) was carried
out on the cryosections. Qualitative and semiquantitative
analyses were performed on the cryosections and semithin
sections. Ultrastructure of myelinated fibers was observed
on the ultrathin sections under electron microscope.
Results: Transplanted SCs and myelinated fibers im-munocytochemically labeled by MBP, P0 as well as S100
distributed in whole injured area. The quantity of myeli-nated fibers labeled by the three myelin proteins showed no
statistical difference, however, which was significantly larger
than that of controls. On the semithin sections, the experi-mental group demonstrated more myelinated fibers in the
injured area than the controls, but the fibers had smaller
diameter and thinner myelin sheath under electron
microscope.
Conclusion: SCs can promote regeneration of injured
nerve fibers and enhance remyelination, which may be his-tological basis of SCs-mediated functional repair of injured
spinal cords.
Key words: Spinal cord injury; Schwann cells; My-elin basic protein; Myelin P0 protein; S100 proteins |
| first_indexed | 2024-12-21T10:09:57Z |
| format | Article |
| id | doaj.art-f926613dbdae4d93b4af3e843537eb18 |
| institution | Directory Open Access Journal |
| issn | 1008-1275 |
| language | English |
| last_indexed | 2024-12-21T10:09:57Z |
| publishDate | 2013-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Chinese Journal of Traumatology |
| spelling | doaj.art-f926613dbdae4d93b4af3e843537eb182022-12-21T19:07:44ZengElsevierChinese Journal of Traumatology1008-12752013-08-01164225229241Morphological study of Schwann cells remyelination in contused spinal cord of ratsLI YueZHANG LuZHANG Jie-yuanLIU ZhengDUAN Zhao-xiaLI Bing-cang【Abstract】Objective: To study the role and effect of Schwann cells (SCs) remyelination in contused spinal cord. Methods: Green fluorescence protein expressing-SCs were transplanted into the epicenter, rostral and caudal tis-sues of the injury site at 1 week after the spinal cords were contused. At 6 weeks, the spinal cords were removed for cryosections, semithin sections and ultrathin sections, and then immunocytochemical staining of myelin basic protein (MBP), P0 protein (P0) and S100 protein (S100) was carried out on the cryosections. Qualitative and semiquantitative analyses were performed on the cryosections and semithin sections. Ultrastructure of myelinated fibers was observed on the ultrathin sections under electron microscope. Results: Transplanted SCs and myelinated fibers im-munocytochemically labeled by MBP, P0 as well as S100 distributed in whole injured area. The quantity of myeli-nated fibers labeled by the three myelin proteins showed no statistical difference, however, which was significantly larger than that of controls. On the semithin sections, the experi-mental group demonstrated more myelinated fibers in the injured area than the controls, but the fibers had smaller diameter and thinner myelin sheath under electron microscope. Conclusion: SCs can promote regeneration of injured nerve fibers and enhance remyelination, which may be his-tological basis of SCs-mediated functional repair of injured spinal cords. Key words: Spinal cord injury; Schwann cells; My-elin basic protein; Myelin P0 protein; S100 proteinshttp://www.cjtrauma.com/apps/ojs/index.php/cjt/article/view/429 |
| spellingShingle | LI Yue ZHANG Lu ZHANG Jie-yuan LIU Zheng DUAN Zhao-xia LI Bing-cang Morphological study of Schwann cells remyelination in contused spinal cord of rats Chinese Journal of Traumatology |
| title | Morphological study of Schwann cells remyelination in contused spinal cord of rats |
| title_full | Morphological study of Schwann cells remyelination in contused spinal cord of rats |
| title_fullStr | Morphological study of Schwann cells remyelination in contused spinal cord of rats |
| title_full_unstemmed | Morphological study of Schwann cells remyelination in contused spinal cord of rats |
| title_short | Morphological study of Schwann cells remyelination in contused spinal cord of rats |
| title_sort | morphological study of schwann cells remyelination in contused spinal cord of rats |
| url | http://www.cjtrauma.com/apps/ojs/index.php/cjt/article/view/429 |
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