Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats
Abstract Molecular methods such as Copro-PCR stand out in the diagnosis of T. gondii, because they are highly sensitive and specific, and can distinguish T. gondii from other morphologically similar coccids. The purpose was the detection of Toxoplasma gondii copro-prevalence by polymerase chain reac...
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Colégio Brasileiro de Parasitologia Veterinaria
2021-05-01
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612021000200301&tlng=en |
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| author | Jaqueline Ataíde Silva Lima da Igreja Hanstter Hallison Alves Rezende Jade de Oliveira Melo João Luís Garcia Felippe Danyel Cardoso Martins Ana Maria de Castro |
| author_facet | Jaqueline Ataíde Silva Lima da Igreja Hanstter Hallison Alves Rezende Jade de Oliveira Melo João Luís Garcia Felippe Danyel Cardoso Martins Ana Maria de Castro |
| author_sort | Jaqueline Ataíde Silva Lima da Igreja |
| collection | DOAJ |
| description | Abstract Molecular methods such as Copro-PCR stand out in the diagnosis of T. gondii, because they are highly sensitive and specific, and can distinguish T. gondii from other morphologically similar coccids. The purpose was the detection of Toxoplasma gondii copro-prevalence by polymerase chain reaction in 149 fecal samples from stray and domiciled cats, using three distinct markers (B5-B6, 18S and 529bp RE). Oocysts of T. gondii/H. hammondi were detected in 15.4% by parasitology fecal tests (PFT), and 4% of these oocysts were positively identified as T. gondii by Copro-PCR. The presence of T. gondii genetic material was detected in 16.1%, but 12% of the samples that tested positive by Copro-PCR were negative in PFT. Samples with discordant results were subjected to a new Copro-PCR with 18S marker and a 529, and of the 17 samples, 9 contained T. gondii genetic material. A comparison of the PFT and the molecular methods showed the latter was more sensitive, since it detected 22.1% while the PFT detected 15.4%. Demonstrating the high sensitivity and specificity of the Copro-PCR, particularly with the association of primers (k=0.809), but also confirms the importance of using molecular techniques in laboratories, since Copro-PCR was able to detect samples considered negative by PFT. |
| first_indexed | 2024-12-19T17:44:45Z |
| format | Article |
| id | doaj.art-f92a1fd69a074ce0a40c0394c11004ee |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-12-19T17:44:45Z |
| publishDate | 2021-05-01 |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-f92a1fd69a074ce0a40c0394c11004ee2022-12-21T20:12:07ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-29612021-05-0130210.1590/s1984-29612021022Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled catsJaqueline Ataíde Silva Lima da Igrejahttps://orcid.org/0000-0001-5743-043XHanstter Hallison Alves RezendeJade de Oliveira MeloJoão Luís GarciaFelippe Danyel Cardoso MartinsAna Maria de CastroAbstract Molecular methods such as Copro-PCR stand out in the diagnosis of T. gondii, because they are highly sensitive and specific, and can distinguish T. gondii from other morphologically similar coccids. The purpose was the detection of Toxoplasma gondii copro-prevalence by polymerase chain reaction in 149 fecal samples from stray and domiciled cats, using three distinct markers (B5-B6, 18S and 529bp RE). Oocysts of T. gondii/H. hammondi were detected in 15.4% by parasitology fecal tests (PFT), and 4% of these oocysts were positively identified as T. gondii by Copro-PCR. The presence of T. gondii genetic material was detected in 16.1%, but 12% of the samples that tested positive by Copro-PCR were negative in PFT. Samples with discordant results were subjected to a new Copro-PCR with 18S marker and a 529, and of the 17 samples, 9 contained T. gondii genetic material. A comparison of the PFT and the molecular methods showed the latter was more sensitive, since it detected 22.1% while the PFT detected 15.4%. Demonstrating the high sensitivity and specificity of the Copro-PCR, particularly with the association of primers (k=0.809), but also confirms the importance of using molecular techniques in laboratories, since Copro-PCR was able to detect samples considered negative by PFT.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612021000200301&tlng=enToxoplasma gondiiCopro-PCRcats |
| spellingShingle | Jaqueline Ataíde Silva Lima da Igreja Hanstter Hallison Alves Rezende Jade de Oliveira Melo João Luís Garcia Felippe Danyel Cardoso Martins Ana Maria de Castro Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats Revista Brasileira de Parasitologia Veterinária Toxoplasma gondii Copro-PCR cats |
| title | Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats |
| title_full | Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats |
| title_fullStr | Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats |
| title_full_unstemmed | Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats |
| title_short | Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats |
| title_sort | copro pcr in the detection and confirmation of toxoplasma gondii oocysts in feces of stray and domiciled cats |
| topic | Toxoplasma gondii Copro-PCR cats |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612021000200301&tlng=en |
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