First report on chlorophyllin to protect mammalian and fish muscle cells from pesticide toxicity via activation of p53 and PARP
Objectives: Pesticide toxicity has become one of the major environmental menaces affecting all types of life forms of the ecosystem. Pesticides get washed off from agricultural fields into nearby water bodies and enter the aquatic organisms. Their bio-accumulated form finally reaches the human race,...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2021-07-01
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| Series: | Aquaculture and Fisheries |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468550X20301015 |
| _version_ | 1797204089273057280 |
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| author | Asmita Samadder Swatilekha Das Bakul Pal Sweta Das Anindita Mandal Priyanka Biswas Sujoy Ghosh Shamim Hossain Mandal Priyanka Sow Ruchira Das Souvik Biswas Ashis Kumar Panigrahi |
| author_facet | Asmita Samadder Swatilekha Das Bakul Pal Sweta Das Anindita Mandal Priyanka Biswas Sujoy Ghosh Shamim Hossain Mandal Priyanka Sow Ruchira Das Souvik Biswas Ashis Kumar Panigrahi |
| author_sort | Asmita Samadder |
| collection | DOAJ |
| description | Objectives: Pesticide toxicity has become one of the major environmental menaces affecting all types of life forms of the ecosystem. Pesticides get washed off from agricultural fields into nearby water bodies and enter the aquatic organisms. Their bio-accumulated form finally reaches the human race, through consumption of pesticide infested aquatic animals, causing several physiological dysfunctions. Hence it becomes necessary to find a therapeutic cure/a preventive measure to stop the health hazard issues of pesticide. With this projection a search for a phyto-based-product was made whose primary objective would be to lower the pesticidal toxicity in fish and simultaneously in the human race. Methods: In this study we tried to check whether the phyto-chemical, Chlorophyllin (CHL), known for its anti-genotoxic, anti-oxidant activities, could render any kind of protection against Cypermethrin (CM) induced-toxicity in fish model and mammalian cell line L6. Both the model L6 and fish were pre-treated with CHL prior to exposure of CM. Different scientific parameters like % cellular cytotoxicity, reactive oxygen species (ROS) generation, nuclear condensation, etc were checked to validate the possibility of CHL in protecting CM-induced toxicity. Results: The overall results revealed that pre-treatment with CHL could restrict the ROS generation leading to modulation in associated cytokine proteins expression NFkβ and IFNγ. Further, CHL lowered nuclear condensation and elevated expression of DNA repair proteins p53 and PARP, showing a kind of pre-activation of signalling cascades for overall protection against the severity of pesticidal toxicity. Conclusion: Thus, this phyto-based preventive approach would possibly solve many areas of human health issues related to pesticide toxicity in future. |
| first_indexed | 2024-04-24T08:29:41Z |
| format | Article |
| id | doaj.art-f92b8a46f45744cc825a1fccded8b6f0 |
| institution | Directory Open Access Journal |
| issn | 2468-550X |
| language | English |
| last_indexed | 2024-04-24T08:29:41Z |
| publishDate | 2021-07-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Aquaculture and Fisheries |
| spelling | doaj.art-f92b8a46f45744cc825a1fccded8b6f02024-04-16T20:31:56ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2021-07-0164393399First report on chlorophyllin to protect mammalian and fish muscle cells from pesticide toxicity via activation of p53 and PARPAsmita Samadder0Swatilekha Das1Bakul Pal2Sweta Das3Anindita Mandal4Priyanka Biswas5Sujoy Ghosh6Shamim Hossain Mandal7Priyanka Sow8Ruchira Das9Souvik Biswas10Ashis Kumar Panigrahi11Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani, Nadia, 741235, India; Corresponding author. Department of Zoology, University of Kalyani, Kalyani, Nadia, 741235, India.Cell and Developmental Biology Special, Department of Zoology, University of Kalyani, Kalyani, Nadia, 741235, IndiaCell and Developmental Biology Special, Department of Zoology, University of Kalyani, Kalyani, Nadia, 741235, IndiaCell and Developmental Biology Special, Department of Zoology, University of Kalyani, Kalyani, Nadia, 741235, IndiaCell and Developmental Biology Special, Department of Zoology, University of Kalyani, Kalyani, Nadia, 741235, IndiaCell and Developmental Biology Special, Department of Zoology, University of Kalyani, Kalyani, Nadia, 741235, IndiaCell and Developmental Biology Special, Department of Zoology, University of Kalyani, Kalyani, Nadia, 741235, IndiaCell and Developmental Biology Special, Department of Zoology, University of Kalyani, Kalyani, Nadia, 741235, IndiaCytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani, Nadia, 741235, IndiaCytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani, Nadia, 741235, India; Department of Zoology, Dum Dum Motijheel College, Kolkata, 700074, IndiaEco-Toxicology, Fisheries and Aquaculture Extn. Laboratory, Department of Zoology, University of Kalyani, Kalyani, Nadia, 741235, IndiaEco-Toxicology, Fisheries and Aquaculture Extn. Laboratory, Department of Zoology, University of Kalyani, Kalyani, Nadia, 741235, IndiaObjectives: Pesticide toxicity has become one of the major environmental menaces affecting all types of life forms of the ecosystem. Pesticides get washed off from agricultural fields into nearby water bodies and enter the aquatic organisms. Their bio-accumulated form finally reaches the human race, through consumption of pesticide infested aquatic animals, causing several physiological dysfunctions. Hence it becomes necessary to find a therapeutic cure/a preventive measure to stop the health hazard issues of pesticide. With this projection a search for a phyto-based-product was made whose primary objective would be to lower the pesticidal toxicity in fish and simultaneously in the human race. Methods: In this study we tried to check whether the phyto-chemical, Chlorophyllin (CHL), known for its anti-genotoxic, anti-oxidant activities, could render any kind of protection against Cypermethrin (CM) induced-toxicity in fish model and mammalian cell line L6. Both the model L6 and fish were pre-treated with CHL prior to exposure of CM. Different scientific parameters like % cellular cytotoxicity, reactive oxygen species (ROS) generation, nuclear condensation, etc were checked to validate the possibility of CHL in protecting CM-induced toxicity. Results: The overall results revealed that pre-treatment with CHL could restrict the ROS generation leading to modulation in associated cytokine proteins expression NFkβ and IFNγ. Further, CHL lowered nuclear condensation and elevated expression of DNA repair proteins p53 and PARP, showing a kind of pre-activation of signalling cascades for overall protection against the severity of pesticidal toxicity. Conclusion: Thus, this phyto-based preventive approach would possibly solve many areas of human health issues related to pesticide toxicity in future.http://www.sciencedirect.com/science/article/pii/S2468550X20301015CypermethrinChlorophyllinFishL6 cellsDNA damageROS generation |
| spellingShingle | Asmita Samadder Swatilekha Das Bakul Pal Sweta Das Anindita Mandal Priyanka Biswas Sujoy Ghosh Shamim Hossain Mandal Priyanka Sow Ruchira Das Souvik Biswas Ashis Kumar Panigrahi First report on chlorophyllin to protect mammalian and fish muscle cells from pesticide toxicity via activation of p53 and PARP Aquaculture and Fisheries Cypermethrin Chlorophyllin Fish L6 cells DNA damage ROS generation |
| title | First report on chlorophyllin to protect mammalian and fish muscle cells from pesticide toxicity via activation of p53 and PARP |
| title_full | First report on chlorophyllin to protect mammalian and fish muscle cells from pesticide toxicity via activation of p53 and PARP |
| title_fullStr | First report on chlorophyllin to protect mammalian and fish muscle cells from pesticide toxicity via activation of p53 and PARP |
| title_full_unstemmed | First report on chlorophyllin to protect mammalian and fish muscle cells from pesticide toxicity via activation of p53 and PARP |
| title_short | First report on chlorophyllin to protect mammalian and fish muscle cells from pesticide toxicity via activation of p53 and PARP |
| title_sort | first report on chlorophyllin to protect mammalian and fish muscle cells from pesticide toxicity via activation of p53 and parp |
| topic | Cypermethrin Chlorophyllin Fish L6 cells DNA damage ROS generation |
| url | http://www.sciencedirect.com/science/article/pii/S2468550X20301015 |
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