VAL34LEU POLYMORPHISM DETECTION BY REAL TIME PCR ASSAY USING FLUORESCENCE RESONANCE ENERGY TRANSFER ON ROTOR-GENE 6000
Factor XIII (FXIII) Val34Leu is the most important polymorphism of the A subunit in factor XIII gene. The aim of the present study was to develop an efficient method based on real time PCR with Fluorescence resonance energy transfer (FRET) detection and melting curve analysis for the detection of...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Rojan GÜMÜŞ
2016-01-01
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| Series: | International Journal of Health Services Research and Policy |
| Subjects: | |
| Online Access: | http://dergipark.gov.tr/ijhsrp/issue/30684/331445 |
| _version_ | 1797918866646499328 |
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| author | Maryam NASER KAMJOO Ali NAZEMI |
| author_facet | Maryam NASER KAMJOO Ali NAZEMI |
| author_sort | Maryam NASER KAMJOO |
| collection | DOAJ |
| description | Factor XIII (FXIII) Val34Leu is the most important polymorphism of the A subunit in factor XIII gene. The aim of the present study was to develop an efficient method based on real time PCR with
Fluorescence resonance energy transfer (FRET) detection and melting curve analysis for the detection of Val34Leu polymorphism.Specific primers were used to amplify the relevant fragment of the factor XIII-A gene and fluorescence resonance energy transfer hybridization probes were used for detection in a Rotor-Gene Q 5Plex HRM platform. Melting temperature (Tm) for the wild type was at 68.8, while Tm for homozygote mutant was at 63.8; heterozygote demonstrated both peaks.Our results showed that primers and probes propoesd in this study demonstrated a high specificity to identify wild type , heterozygous and
homozygous mutantgenotypes. Due to the increasing molecular diagnosis in developing countries and the importance of identifying polymorphisms, this real time PCR assay is of great importance. An important advantage of this approach is the high sensitivity and specificity. |
| first_indexed | 2024-04-10T13:37:20Z |
| format | Article |
| id | doaj.art-f92cb346f3fd4acd8aafc75126e4a2af |
| institution | Directory Open Access Journal |
| issn | 2602-3482 |
| language | English |
| last_indexed | 2024-04-10T13:37:20Z |
| publishDate | 2016-01-01 |
| publisher | Rojan GÜMÜŞ |
| record_format | Article |
| series | International Journal of Health Services Research and Policy |
| spelling | doaj.art-f92cb346f3fd4acd8aafc75126e4a2af2023-02-15T16:11:22ZengRojan GÜMÜŞInternational Journal of Health Services Research and Policy2602-34822016-01-0111151910.23884/ijhsrp.2016.1.1.02VAL34LEU POLYMORPHISM DETECTION BY REAL TIME PCR ASSAY USING FLUORESCENCE RESONANCE ENERGY TRANSFER ON ROTOR-GENE 6000Maryam NASER KAMJOO0Ali NAZEMI1Islamic Azad University/IRANIslamic Azad University/IRANFactor XIII (FXIII) Val34Leu is the most important polymorphism of the A subunit in factor XIII gene. The aim of the present study was to develop an efficient method based on real time PCR with Fluorescence resonance energy transfer (FRET) detection and melting curve analysis for the detection of Val34Leu polymorphism.Specific primers were used to amplify the relevant fragment of the factor XIII-A gene and fluorescence resonance energy transfer hybridization probes were used for detection in a Rotor-Gene Q 5Plex HRM platform. Melting temperature (Tm) for the wild type was at 68.8, while Tm for homozygote mutant was at 63.8; heterozygote demonstrated both peaks.Our results showed that primers and probes propoesd in this study demonstrated a high specificity to identify wild type , heterozygous and homozygous mutantgenotypes. Due to the increasing molecular diagnosis in developing countries and the importance of identifying polymorphisms, this real time PCR assay is of great importance. An important advantage of this approach is the high sensitivity and specificity.http://dergipark.gov.tr/ijhsrp/issue/30684/331445factor XIIIval34leuFluorescence resonance energy transfer |
| spellingShingle | Maryam NASER KAMJOO Ali NAZEMI VAL34LEU POLYMORPHISM DETECTION BY REAL TIME PCR ASSAY USING FLUORESCENCE RESONANCE ENERGY TRANSFER ON ROTOR-GENE 6000 International Journal of Health Services Research and Policy factor XIII val34leu Fluorescence resonance energy transfer |
| title | VAL34LEU POLYMORPHISM DETECTION BY REAL TIME PCR ASSAY USING FLUORESCENCE RESONANCE ENERGY TRANSFER ON ROTOR-GENE 6000 |
| title_full | VAL34LEU POLYMORPHISM DETECTION BY REAL TIME PCR ASSAY USING FLUORESCENCE RESONANCE ENERGY TRANSFER ON ROTOR-GENE 6000 |
| title_fullStr | VAL34LEU POLYMORPHISM DETECTION BY REAL TIME PCR ASSAY USING FLUORESCENCE RESONANCE ENERGY TRANSFER ON ROTOR-GENE 6000 |
| title_full_unstemmed | VAL34LEU POLYMORPHISM DETECTION BY REAL TIME PCR ASSAY USING FLUORESCENCE RESONANCE ENERGY TRANSFER ON ROTOR-GENE 6000 |
| title_short | VAL34LEU POLYMORPHISM DETECTION BY REAL TIME PCR ASSAY USING FLUORESCENCE RESONANCE ENERGY TRANSFER ON ROTOR-GENE 6000 |
| title_sort | val34leu polymorphism detection by real time pcr assay using fluorescence resonance energy transfer on rotor gene 6000 |
| topic | factor XIII val34leu Fluorescence resonance energy transfer |
| url | http://dergipark.gov.tr/ijhsrp/issue/30684/331445 |
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