Effects of ATP on the Physicochemical Properties and Cytocompatibility of Calcium Sulfate/Calcium Citrate Composite Cement
Adenosine triphosphate (ATP), acting as a source of energy, has effects on cellular activities, such as adhesion, proliferation, and differentiation. In this study, ATP-loaded calcium sulfate hemihydrate/calcium citrate tetrahydrate cement (ATP/CSH/CCT) was successfully prepared for the first time....
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/1996-1944/16/11/3947 |
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| author | Xiangyue Liu Hong Chen Haohao Ren Bo Wang Xiaodan Li Suping Peng Qiyi Zhang Yonggang Yan |
| author_facet | Xiangyue Liu Hong Chen Haohao Ren Bo Wang Xiaodan Li Suping Peng Qiyi Zhang Yonggang Yan |
| author_sort | Xiangyue Liu |
| collection | DOAJ |
| description | Adenosine triphosphate (ATP), acting as a source of energy, has effects on cellular activities, such as adhesion, proliferation, and differentiation. In this study, ATP-loaded calcium sulfate hemihydrate/calcium citrate tetrahydrate cement (ATP/CSH/CCT) was successfully prepared for the first time. The effect of different contents of ATP on the structure and physicochemical properties of ATP/CSH/CCT was also studied in detail. The results indicated that incorporating ATP into the cement did not significantly alter their structures. However, the addition ratio of ATP directly impacted the mechanical properties and in vitro degradation properties of the composite bone cement. The compressive strength of ATP/CSH/CCT gradually decreased with an increasing ATP content. The degradation rate of ATP/CSH/CCT did not significantly change at low concentrations of ATP, but it increased with a higher ATP content. The composite cement induced the deposition of a Ca-P layer in a phosphate buffer solution (PBS, pH = 7.4). Additionally, the release of ATP from the composite cement was controlled. The ATP was controlled releasing at the 0.5% and 1% ATP in cement by the diffusion of ATP and the degradation of the cement, whereas it was controlled by the diffusion process merely at the 0.1% ATP in cement. Furthermore, ATP/CSH/CCT demonstrated good cytoactivity with the addition of ATP and is expected to be used for the repair and regeneration of bone tissue. |
| first_indexed | 2024-03-11T03:04:14Z |
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| id | doaj.art-f9309c94204349d7b9b7dd7cd00388c8 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-11T03:04:14Z |
| publishDate | 2023-05-01 |
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| spelling | doaj.art-f9309c94204349d7b9b7dd7cd00388c82023-11-18T08:08:12ZengMDPI AGMaterials1996-19442023-05-011611394710.3390/ma16113947Effects of ATP on the Physicochemical Properties and Cytocompatibility of Calcium Sulfate/Calcium Citrate Composite CementXiangyue Liu0Hong Chen1Haohao Ren2Bo Wang3Xiaodan Li4Suping Peng5Qiyi Zhang6Yonggang Yan7College of Physics, Sichuan University, Chengdu 610065, ChinaCollege of Physics, Sichuan University, Chengdu 610065, ChinaCollege of Physics, Sichuan University, Chengdu 610065, ChinaSchool of Chemical Engineering, Sichuan University, Chengdu 610065, ChinaCollege of Physics, Sichuan University, Chengdu 610065, ChinaSchool of Chemical Engineering, Sichuan University, Chengdu 610065, ChinaSchool of Chemical Engineering, Sichuan University, Chengdu 610065, ChinaCollege of Physics, Sichuan University, Chengdu 610065, ChinaAdenosine triphosphate (ATP), acting as a source of energy, has effects on cellular activities, such as adhesion, proliferation, and differentiation. In this study, ATP-loaded calcium sulfate hemihydrate/calcium citrate tetrahydrate cement (ATP/CSH/CCT) was successfully prepared for the first time. The effect of different contents of ATP on the structure and physicochemical properties of ATP/CSH/CCT was also studied in detail. The results indicated that incorporating ATP into the cement did not significantly alter their structures. However, the addition ratio of ATP directly impacted the mechanical properties and in vitro degradation properties of the composite bone cement. The compressive strength of ATP/CSH/CCT gradually decreased with an increasing ATP content. The degradation rate of ATP/CSH/CCT did not significantly change at low concentrations of ATP, but it increased with a higher ATP content. The composite cement induced the deposition of a Ca-P layer in a phosphate buffer solution (PBS, pH = 7.4). Additionally, the release of ATP from the composite cement was controlled. The ATP was controlled releasing at the 0.5% and 1% ATP in cement by the diffusion of ATP and the degradation of the cement, whereas it was controlled by the diffusion process merely at the 0.1% ATP in cement. Furthermore, ATP/CSH/CCT demonstrated good cytoactivity with the addition of ATP and is expected to be used for the repair and regeneration of bone tissue.https://www.mdpi.com/1996-1944/16/11/3947ATPcalcium sulfate hemihydratecalcium citrate tetrahydratecomposite bone cementcytoactivity |
| spellingShingle | Xiangyue Liu Hong Chen Haohao Ren Bo Wang Xiaodan Li Suping Peng Qiyi Zhang Yonggang Yan Effects of ATP on the Physicochemical Properties and Cytocompatibility of Calcium Sulfate/Calcium Citrate Composite Cement Materials ATP calcium sulfate hemihydrate calcium citrate tetrahydrate composite bone cement cytoactivity |
| title | Effects of ATP on the Physicochemical Properties and Cytocompatibility of Calcium Sulfate/Calcium Citrate Composite Cement |
| title_full | Effects of ATP on the Physicochemical Properties and Cytocompatibility of Calcium Sulfate/Calcium Citrate Composite Cement |
| title_fullStr | Effects of ATP on the Physicochemical Properties and Cytocompatibility of Calcium Sulfate/Calcium Citrate Composite Cement |
| title_full_unstemmed | Effects of ATP on the Physicochemical Properties and Cytocompatibility of Calcium Sulfate/Calcium Citrate Composite Cement |
| title_short | Effects of ATP on the Physicochemical Properties and Cytocompatibility of Calcium Sulfate/Calcium Citrate Composite Cement |
| title_sort | effects of atp on the physicochemical properties and cytocompatibility of calcium sulfate calcium citrate composite cement |
| topic | ATP calcium sulfate hemihydrate calcium citrate tetrahydrate composite bone cement cytoactivity |
| url | https://www.mdpi.com/1996-1944/16/11/3947 |
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