Systematic evaluation of signal-to-noise ratio in variant detection from single cell genome multiple displacement amplification and exome sequencing

Systematic evaluation of signal-to-noise ratio in variant detection from single cell genome multiple displacement amplification and exome sequencing

Abstract Background The current literature on single cell genomic analyses on the DNA level is conflicting regarding requirements for cell quality, amplification success rates, allelic dropouts and resolution, lacking a systematic comparison of multiple cell input down to the single cell. We hypothe...

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Bibliographic Details
Main Authors: Anita T. Simonsen, Marcus C. Hansen, Eigil Kjeldsen, Peter L. Møller, Johnny J. Hindkjær, Peter Hokland, Anni Aggerholm
Format: Article
Language:English
Published: BMC 2018-09-01
Series:BMC Genomics
Subjects:
Single cell sequencing
Sparse cell sequencing
Allele dropout
Signal-to-noise ratio
Whole exome sequencing
NGS
Online Access:http://link.springer.com/article/10.1186/s12864-018-5063-5

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