Mutagenic and DNA repair activity in traffic policemen: a case-crossover study
Abstract Background Emissions from vehicles are composed of heterogeneous mixtures of hazardous substances; several pollutants such as Polycyclic Aromatic Hydrocarbons (PAHs) are amongst the most dangerous substances detected in urban monitoring. A cohort of traffic policemen usually occupationally...
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| Format: | Article |
| Language: | English |
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BMC
2018-08-01
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| Series: | Journal of Occupational Medicine and Toxicology |
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| Online Access: | http://link.springer.com/article/10.1186/s12995-018-0206-9 |
| _version_ | 1828136470018260992 |
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| author | Caterina Ledda Carla Loreto Massimo Bracci Claudia Lombardo Gaetano Romano Diana Cinà Nicola Mucci Sergio Castorina Venerando Rapisarda |
| author_facet | Caterina Ledda Carla Loreto Massimo Bracci Claudia Lombardo Gaetano Romano Diana Cinà Nicola Mucci Sergio Castorina Venerando Rapisarda |
| author_sort | Caterina Ledda |
| collection | DOAJ |
| description | Abstract Background Emissions from vehicles are composed of heterogeneous mixtures of hazardous substances; several pollutants such as Polycyclic Aromatic Hydrocarbons (PAHs) are amongst the most dangerous substances detected in urban monitoring. A cohort of traffic policemen usually occupationally exposed to PAHs present in the urban environment were examined in order to assess the mutagenicity and DNA capacity repair. Methods Seventy-two urban traffic policemen working in Catania’s metropolitan area were enrolled in the study. Two spot urine samples were collected from each subject during the whole working cycle as follows: sample 1 (S1), pre-shift on day 1; sample 2 (S2) post-shift on day 6. 1-hydroxypyrene (1-OHP) was measured to serve as an indirect exposure indicator. Urinary mutagenic activity was assessed through the plate incorporation pre-incubation technique with S9, using YG1024 Salmonella typhimurium strain over-sensitive to PAH metabolite. Concentrations of urinary 8-oxodG were measured using liquid chromatography tandem mass spectrometry. Results As regards the exposure to PAHs, results highlighted a statistically significant difference (p < 0.001) between pre-shift on day 1 and post-shift on day 6 levels. Mutagenic activity was detected in 38 (66%) workers on S1 and in 47 (81%) on S2. Also 8-oxodG analysis showed a statistically significant difference between S1 and S2 sampling. Conclusions This study demonstrated that occupational exposure to pollutants from traffic emission, assessed via 1-OHP measurements in urine, may lead to DNA repair and mutagenic activity, in line with other studies. |
| first_indexed | 2024-04-11T18:04:48Z |
| format | Article |
| id | doaj.art-f93324ce8d434a15b32b47df0d1ef323 |
| institution | Directory Open Access Journal |
| issn | 1745-6673 |
| language | English |
| last_indexed | 2024-04-11T18:04:48Z |
| publishDate | 2018-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Occupational Medicine and Toxicology |
| spelling | doaj.art-f93324ce8d434a15b32b47df0d1ef3232022-12-22T04:10:22ZengBMCJournal of Occupational Medicine and Toxicology1745-66732018-08-011311710.1186/s12995-018-0206-9Mutagenic and DNA repair activity in traffic policemen: a case-crossover studyCaterina Ledda0Carla Loreto1Massimo Bracci2Claudia Lombardo3Gaetano Romano4Diana Cinà5Nicola Mucci6Sergio Castorina7Venerando Rapisarda8Occupational Medicine, Department of Clinical and Experimental Medicine, University of CataniaHuman Anatomy and Histology, Department of Biomedical and Biotechnology Sciences, University of CataniaOccupational Medicine, Department of Clinical and Molecular Sciences, Polytechnic University of MarcheHuman Anatomy and Histology, Department of Biomedical and Biotechnology Sciences, University of CataniaOccupational Medicine, Department of Clinical and Experimental Medicine, University of CataniaClinical Pathology Unit, “Garibaldi Centro” Hospital of CataniaOccupational Medicine, Department of Experimental and Clinical Medicine, University of FlorenceHuman Anatomy and Histology, Department of Biomedical and Biotechnology Sciences, University of CataniaOccupational Medicine, Department of Clinical and Experimental Medicine, University of CataniaAbstract Background Emissions from vehicles are composed of heterogeneous mixtures of hazardous substances; several pollutants such as Polycyclic Aromatic Hydrocarbons (PAHs) are amongst the most dangerous substances detected in urban monitoring. A cohort of traffic policemen usually occupationally exposed to PAHs present in the urban environment were examined in order to assess the mutagenicity and DNA capacity repair. Methods Seventy-two urban traffic policemen working in Catania’s metropolitan area were enrolled in the study. Two spot urine samples were collected from each subject during the whole working cycle as follows: sample 1 (S1), pre-shift on day 1; sample 2 (S2) post-shift on day 6. 1-hydroxypyrene (1-OHP) was measured to serve as an indirect exposure indicator. Urinary mutagenic activity was assessed through the plate incorporation pre-incubation technique with S9, using YG1024 Salmonella typhimurium strain over-sensitive to PAH metabolite. Concentrations of urinary 8-oxodG were measured using liquid chromatography tandem mass spectrometry. Results As regards the exposure to PAHs, results highlighted a statistically significant difference (p < 0.001) between pre-shift on day 1 and post-shift on day 6 levels. Mutagenic activity was detected in 38 (66%) workers on S1 and in 47 (81%) on S2. Also 8-oxodG analysis showed a statistically significant difference between S1 and S2 sampling. Conclusions This study demonstrated that occupational exposure to pollutants from traffic emission, assessed via 1-OHP measurements in urine, may lead to DNA repair and mutagenic activity, in line with other studies.http://link.springer.com/article/10.1186/s12995-018-0206-9PAHsDNA damageOxidative stressAir pollutionUrban trafficCancer |
| spellingShingle | Caterina Ledda Carla Loreto Massimo Bracci Claudia Lombardo Gaetano Romano Diana Cinà Nicola Mucci Sergio Castorina Venerando Rapisarda Mutagenic and DNA repair activity in traffic policemen: a case-crossover study Journal of Occupational Medicine and Toxicology PAHs DNA damage Oxidative stress Air pollution Urban traffic Cancer |
| title | Mutagenic and DNA repair activity in traffic policemen: a case-crossover study |
| title_full | Mutagenic and DNA repair activity in traffic policemen: a case-crossover study |
| title_fullStr | Mutagenic and DNA repair activity in traffic policemen: a case-crossover study |
| title_full_unstemmed | Mutagenic and DNA repair activity in traffic policemen: a case-crossover study |
| title_short | Mutagenic and DNA repair activity in traffic policemen: a case-crossover study |
| title_sort | mutagenic and dna repair activity in traffic policemen a case crossover study |
| topic | PAHs DNA damage Oxidative stress Air pollution Urban traffic Cancer |
| url | http://link.springer.com/article/10.1186/s12995-018-0206-9 |
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