Molecular detection of Salmonella spp., Listeria spp. and Brucella spp. in fresh artisanal cheese marketed in the city of Barranquilla: A pilot study
Introduction: Each year approximately 3 million people die as the result of foodborne diseases. The fresh artisan (handmade) cheese produced and distributed in the Colombian Caribbean region is a native product from the departments of Córdoba, Sucre, Bolívar, Atlántico, Magdalena, Cesar, and La Guaj...
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| Format: | Article |
| Language: | English |
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Instituto Nacional de Salud
2018-08-01
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| Series: | Biomédica: revista del Instituto Nacional de Salud |
| Subjects: | |
| Online Access: | https://www.revistabiomedica.org/index.php/biomedica/article/view/3677 |
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| author | Zamira E. Soto-Varela Clara Gilma Gutiérrez Yurina de Moya Ramón Mattos Hernando José Bolívar-Anillo José Luis Villarreal |
| author_facet | Zamira E. Soto-Varela Clara Gilma Gutiérrez Yurina de Moya Ramón Mattos Hernando José Bolívar-Anillo José Luis Villarreal |
| author_sort | Zamira E. Soto-Varela |
| collection | DOAJ |
| description | Introduction: Each year approximately 3 million people die as the result of foodborne diseases. The fresh artisan (handmade) cheese produced and distributed in the Colombian Caribbean region is a native product from the departments of Córdoba, Sucre, Bolívar, Atlántico, Magdalena, Cesar, and La Guajira. Its mass consumption increases the risk of infection with Salmonella spp., Listeria spp., and Brucella spp., as it is made with a very rustic technology, with unpasteurized cow milk, without standardized and hygienic procedures and its storage is inadequate.
Objective: To detect the presence of Salmonella spp., Listeria spp., and Brucella spp. in samples of fresh artisan cheese from the Colombian Caribbean region.
Materials and methods: Twenty-seven samples of cheese from five departments of the Caribbean Region (Atlántico (n=6), Bolívar (n=2), Córdoba (n=1), Magdalena (n=16), and Sucre (n=2)) were analyzed by real-time polymerase chain reaction (qPCR). Seventeen of the samples corresponded to soft cheese, five to semi-hard cheese and five to hard cheese.
Results: In 62.9% (17/27) of the samples we detected Salmonella spp., in 70.4% (19/27), Listeria spp., and in 22.2% (6/27), Brucella spp., mainly from the department of Magdalena. In 62.5% (10/16) of the samples we detected Salmonella spp. and Listeria spp. while in the department of Atlántico, 50% (3/6) of the samples corresponded to Brucella spp.
Conclusion: The results confirmed the presence of these microorganisms in all the samples of soft cheese from the Colombian Caribbean region. |
| first_indexed | 2024-12-10T17:36:03Z |
| format | Article |
| id | doaj.art-f93582fc2e4e416ba3224b86fb777fb6 |
| institution | Directory Open Access Journal |
| issn | 0120-4157 0120-4157 |
| language | English |
| last_indexed | 2024-12-10T17:36:03Z |
| publishDate | 2018-08-01 |
| publisher | Instituto Nacional de Salud |
| record_format | Article |
| series | Biomédica: revista del Instituto Nacional de Salud |
| spelling | doaj.art-f93582fc2e4e416ba3224b86fb777fb62022-12-22T01:39:32ZengInstituto Nacional de SaludBiomédica: revista del Instituto Nacional de Salud0120-41570120-41572018-08-01380303610.7705/biomedica.v38i3.36772119Molecular detection of Salmonella spp., Listeria spp. and Brucella spp. in fresh artisanal cheese marketed in the city of Barranquilla: A pilot studyZamira E. Soto-Varela0Clara Gilma Gutiérrez1Yurina de Moya2Ramón Mattos3Hernando José Bolívar-Anillo4José Luis Villarreal5Grupo de Investigación en Gestión Ecológica y Agroindustrial, Programa de Microbiología, Facultad de Ciencias Exactas, Universidad Libre, Barranquilla, ColombiaGrupo de Investigación en Gestión Ecológica y Agroindustrial, Programa de Microbiología, Facultad de Ciencias Exactas, Universidad Libre, Barranquilla, ColombiaGrupo de Investigación en Gestión Ecológica y Agroindustrial, Programa de Microbiología, Facultad de Ciencias Exactas, Universidad Libre, Barranquilla, ColombiaGrupo de Investigación en Métodos Estadísticos Aplicados, Universidad Libre, Barranquilla, ColombiaGrupo de Investigación de Biomembranas, Programa de Medicina, Facultad de Ciencias de la Salud, Universidad Libre, Barranquilla, ColombiaGrupo de Investigación en Bioquímica Patológica, Programa de Medicina, Facultad de Ciencias de la Salud, Universidad Libre, Barranquilla, ColombiaIntroduction: Each year approximately 3 million people die as the result of foodborne diseases. The fresh artisan (handmade) cheese produced and distributed in the Colombian Caribbean region is a native product from the departments of Córdoba, Sucre, Bolívar, Atlántico, Magdalena, Cesar, and La Guajira. Its mass consumption increases the risk of infection with Salmonella spp., Listeria spp., and Brucella spp., as it is made with a very rustic technology, with unpasteurized cow milk, without standardized and hygienic procedures and its storage is inadequate. Objective: To detect the presence of Salmonella spp., Listeria spp., and Brucella spp. in samples of fresh artisan cheese from the Colombian Caribbean region. Materials and methods: Twenty-seven samples of cheese from five departments of the Caribbean Region (Atlántico (n=6), Bolívar (n=2), Córdoba (n=1), Magdalena (n=16), and Sucre (n=2)) were analyzed by real-time polymerase chain reaction (qPCR). Seventeen of the samples corresponded to soft cheese, five to semi-hard cheese and five to hard cheese. Results: In 62.9% (17/27) of the samples we detected Salmonella spp., in 70.4% (19/27), Listeria spp., and in 22.2% (6/27), Brucella spp., mainly from the department of Magdalena. In 62.5% (10/16) of the samples we detected Salmonella spp. and Listeria spp. while in the department of Atlántico, 50% (3/6) of the samples corresponded to Brucella spp. Conclusion: The results confirmed the presence of these microorganisms in all the samples of soft cheese from the Colombian Caribbean region.https://www.revistabiomedica.org/index.php/biomedica/article/view/3677SalmonellaListeriaBrucellaenfermedades transmitidas por los alimentos/ epidemiologíareacción en cadena de la polimerasaqueso |
| spellingShingle | Zamira E. Soto-Varela Clara Gilma Gutiérrez Yurina de Moya Ramón Mattos Hernando José Bolívar-Anillo José Luis Villarreal Molecular detection of Salmonella spp., Listeria spp. and Brucella spp. in fresh artisanal cheese marketed in the city of Barranquilla: A pilot study Biomédica: revista del Instituto Nacional de Salud Salmonella Listeria Brucella enfermedades transmitidas por los alimentos/ epidemiología reacción en cadena de la polimerasa queso |
| title | Molecular detection of Salmonella spp., Listeria spp. and Brucella spp. in fresh artisanal cheese marketed in the city of Barranquilla: A pilot study |
| title_full | Molecular detection of Salmonella spp., Listeria spp. and Brucella spp. in fresh artisanal cheese marketed in the city of Barranquilla: A pilot study |
| title_fullStr | Molecular detection of Salmonella spp., Listeria spp. and Brucella spp. in fresh artisanal cheese marketed in the city of Barranquilla: A pilot study |
| title_full_unstemmed | Molecular detection of Salmonella spp., Listeria spp. and Brucella spp. in fresh artisanal cheese marketed in the city of Barranquilla: A pilot study |
| title_short | Molecular detection of Salmonella spp., Listeria spp. and Brucella spp. in fresh artisanal cheese marketed in the city of Barranquilla: A pilot study |
| title_sort | molecular detection of salmonella spp listeria spp and brucella spp in fresh artisanal cheese marketed in the city of barranquilla a pilot study |
| topic | Salmonella Listeria Brucella enfermedades transmitidas por los alimentos/ epidemiología reacción en cadena de la polimerasa queso |
| url | https://www.revistabiomedica.org/index.php/biomedica/article/view/3677 |
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