Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb <i>Wedelia chinensis</i>
<i>Wedelia chinensis</i>, which belongs to the Asteraceae family, is a procumbent, perennial herb. It has medicinal anti-inflammatory properties and has been traditionally used as folk medicine in East and South Asia for treating fever, cough and phlegm. In Taiwan, <i>W. chinensis&...
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MDPI AG
2021-06-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/6/1232 |
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| author | Yung-Ting Tsai Po-Yen Chen Kin-Ying To |
| author_facet | Yung-Ting Tsai Po-Yen Chen Kin-Ying To |
| author_sort | Yung-Ting Tsai |
| collection | DOAJ |
| description | <i>Wedelia chinensis</i>, which belongs to the Asteraceae family, is a procumbent, perennial herb. It has medicinal anti-inflammatory properties and has been traditionally used as folk medicine in East and South Asia for treating fever, cough and phlegm. In Taiwan, <i>W. chinensis</i> is a common ingredient of herbal tea. Previous studies showed that the plant leaves contain four major bioactive compounds, wedelolactone, demethylwedelolactone, luteolin and apigenin, that have potent antihepatoxic activity, and are thus used as major ingredients in phytopharmaceutical formulations. In this study, we set up optimal conditions for induction of ploidy in <i>W.</i> <i>chinensis</i>. Ploidy can be an effective method of increasing plant biomass and improving medicinal and ornamental characteristics. By using flow cytometry and chicken erythrocyte nuclei as a reference, the DNA content (2C) or genome size of <i>W. chinensis</i> was determined to be 4.80 picograms (pg) in this study for the first time. Subsequently, we developed the successful induction of five triploid and three tetraploid plants by using shoot explants treated with different concentrations (0, 0.25, 0.5, 1, 1.5, 2 g/L) of colchicine. No apparent morphological changes were observed between these polyploid plants and the diploid wild-type (WT) plant, except that larger stomata in leaves were found in all polyploid plants as compared to diploid WT. Ultra-performance liquid chromatography coupled with tandem mass spectrometry was used to quantify the four index compounds (wedelolactone, demethylwedelolactone, luteolin, apigenin) in these polyploid plants, and fluctuating patterns were detected. This is the first report regarding polyploidy in the herbal plant <i>W. chinensis</i>. |
| first_indexed | 2024-03-10T10:20:04Z |
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| last_indexed | 2024-03-10T10:20:04Z |
| publishDate | 2021-06-01 |
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| series | Plants |
| spelling | doaj.art-f93a7af066d3467c926cb59d6e13af512023-11-22T00:30:49ZengMDPI AGPlants2223-77472021-06-01106123210.3390/plants10061232Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb <i>Wedelia chinensis</i>Yung-Ting Tsai0Po-Yen Chen1Kin-Ying To2Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, TaiwanAgricultural Biotechnology Research Center, Academia Sinica, Taipei 115, TaiwanAgricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan<i>Wedelia chinensis</i>, which belongs to the Asteraceae family, is a procumbent, perennial herb. It has medicinal anti-inflammatory properties and has been traditionally used as folk medicine in East and South Asia for treating fever, cough and phlegm. In Taiwan, <i>W. chinensis</i> is a common ingredient of herbal tea. Previous studies showed that the plant leaves contain four major bioactive compounds, wedelolactone, demethylwedelolactone, luteolin and apigenin, that have potent antihepatoxic activity, and are thus used as major ingredients in phytopharmaceutical formulations. In this study, we set up optimal conditions for induction of ploidy in <i>W.</i> <i>chinensis</i>. Ploidy can be an effective method of increasing plant biomass and improving medicinal and ornamental characteristics. By using flow cytometry and chicken erythrocyte nuclei as a reference, the DNA content (2C) or genome size of <i>W. chinensis</i> was determined to be 4.80 picograms (pg) in this study for the first time. Subsequently, we developed the successful induction of five triploid and three tetraploid plants by using shoot explants treated with different concentrations (0, 0.25, 0.5, 1, 1.5, 2 g/L) of colchicine. No apparent morphological changes were observed between these polyploid plants and the diploid wild-type (WT) plant, except that larger stomata in leaves were found in all polyploid plants as compared to diploid WT. Ultra-performance liquid chromatography coupled with tandem mass spectrometry was used to quantify the four index compounds (wedelolactone, demethylwedelolactone, luteolin, apigenin) in these polyploid plants, and fluctuating patterns were detected. This is the first report regarding polyploidy in the herbal plant <i>W. chinensis</i>.https://www.mdpi.com/2223-7747/10/6/1232colchicineflow cytometryinduction of ploidymedicinal plantmetabolic profilingpolyploid plants |
| spellingShingle | Yung-Ting Tsai Po-Yen Chen Kin-Ying To Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb <i>Wedelia chinensis</i> Plants colchicine flow cytometry induction of ploidy medicinal plant metabolic profiling polyploid plants |
| title | Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb <i>Wedelia chinensis</i> |
| title_full | Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb <i>Wedelia chinensis</i> |
| title_fullStr | Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb <i>Wedelia chinensis</i> |
| title_full_unstemmed | Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb <i>Wedelia chinensis</i> |
| title_short | Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb <i>Wedelia chinensis</i> |
| title_sort | induction of polyploidy and metabolic profiling in the medicinal herb i wedelia chinensis i |
| topic | colchicine flow cytometry induction of ploidy medicinal plant metabolic profiling polyploid plants |
| url | https://www.mdpi.com/2223-7747/10/6/1232 |
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