The Effect Of Lactobacillus Paracasei Isolated From Yoghurt On The Growth And Production Of Staphylococcus Aureus Enterotoxin
Background and Aim: Probiotics are useful bacteria which, after being consumed, leave useful effects on human health. The present study was conducted to examine the antimicrobial effect of Lactobacillus paracasei on pathogenic bacteria, especially on Staphylococcus aureus in the f...
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| Format: | Article |
| Language: | fas |
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Tehran University of Medical Sciences
2014-09-01
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| Series: | پیاورد سلامت |
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| Online Access: | http://payavard.tums.ac.ir/browse.php?a_code=A-10-1-75&slc_lang=en&sid=1 |
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| author | Mahnoosh Parsaeimehr Ali Misaghi Afshin Akhondzade Ashkan Jebelli Javan Mehdi Taheri Nabi Shariatifar |
| author_facet | Mahnoosh Parsaeimehr Ali Misaghi Afshin Akhondzade Ashkan Jebelli Javan Mehdi Taheri Nabi Shariatifar |
| author_sort | Mahnoosh Parsaeimehr |
| collection | DOAJ |
| description | Background and Aim: Probiotics are useful bacteria which, after being
consumed, leave useful effects on human health. The present study was conducted to examine the
antimicrobial effect of Lactobacillus
paracasei on
pathogenic bacteria, especially on Staphylococcus aureus in the field of food microbiology. Materials and Methods: In this study, the effect of Lactobacillus paracasei isolated from
yoghurt (1×108 cfu/ml) on the
growth of Staphylococcus aureus (1×105 cfu/ml)
was assessed at two different temperatures of 25 °C and 35°C in TSB. Then, samples from cocultures and control
were pour plated in Baird Parker agar and MRS agar to compute the number of S.
aureus and Lactobacillus paracasei at 0, 24, 48, 72 hours after incubation.
Then, enterotoxin production was evaluated by ELIZA rida screen kit. The data
were investigated by ANOVA and K-square (chi-square) tests. Results: It was found that compared to controls, Lactobacillus
paracasei inhibited the growth of Staphylococcus aureus and reduced the number
of S. aureus cells about 3 logs in 25°C and 2 logs in 35°C. The production of A, C, and E enterotoxins was
inhibited by S. aureus at a temperature of 25°C according to ELIZA rida screen test. The figure was
35°C for enterotoxins
E and A. Conclusion: Probiotics can be used as a new approach for the
biocontrol of S. aureus without using antibiotics. |
| first_indexed | 2024-12-17T19:53:46Z |
| format | Article |
| id | doaj.art-f93d28cffabd466ab7471a8d061adbc0 |
| institution | Directory Open Access Journal |
| issn | 1735-8132 2008-2665 |
| language | fas |
| last_indexed | 2024-12-17T19:53:46Z |
| publishDate | 2014-09-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | پیاورد سلامت |
| spelling | doaj.art-f93d28cffabd466ab7471a8d061adbc02022-12-21T21:34:40ZfasTehran University of Medical Sciencesپیاورد سلامت1735-81322008-26652014-09-0183188197The Effect Of Lactobacillus Paracasei Isolated From Yoghurt On The Growth And Production Of Staphylococcus Aureus EnterotoxinMahnoosh Parsaeimehr0Ali Misaghi1Afshin Akhondzade2Ashkan Jebelli Javan3Mehdi Taheri4Nabi Shariatifar5 Assistant Professor, Food Hygiene Department, School of Veterinary Medicine, Semnan University, Semnan, Iran Associate Professor, Food Hygiene Department, School of Veterinary Medicine, University of Tehran, Tehran, Iran Professor, Food Hygiene Department, School of Veterinary Medicine, University of Tehran, Tehran, Iran Assistant Professor, Food Hygiene Department, School of Veterinary Medicine, Semnan University, Semnan, Iran Assistant Professor, Food Hygiene Department, School of Veterinary Medicine, University of Tehran, Tehran, Iran Assistant Professor, Environmental Health Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Background and Aim: Probiotics are useful bacteria which, after being consumed, leave useful effects on human health. The present study was conducted to examine the antimicrobial effect of Lactobacillus paracasei on pathogenic bacteria, especially on Staphylococcus aureus in the field of food microbiology. Materials and Methods: In this study, the effect of Lactobacillus paracasei isolated from yoghurt (1×108 cfu/ml) on the growth of Staphylococcus aureus (1×105 cfu/ml) was assessed at two different temperatures of 25 °C and 35°C in TSB. Then, samples from cocultures and control were pour plated in Baird Parker agar and MRS agar to compute the number of S. aureus and Lactobacillus paracasei at 0, 24, 48, 72 hours after incubation. Then, enterotoxin production was evaluated by ELIZA rida screen kit. The data were investigated by ANOVA and K-square (chi-square) tests. Results: It was found that compared to controls, Lactobacillus paracasei inhibited the growth of Staphylococcus aureus and reduced the number of S. aureus cells about 3 logs in 25°C and 2 logs in 35°C. The production of A, C, and E enterotoxins was inhibited by S. aureus at a temperature of 25°C according to ELIZA rida screen test. The figure was 35°C for enterotoxins E and A. Conclusion: Probiotics can be used as a new approach for the biocontrol of S. aureus without using antibiotics. http://payavard.tums.ac.ir/browse.php?a_code=A-10-1-75&slc_lang=en&sid=1lactobacillus paracasei enterotoxin staphylococcus aureus |
| spellingShingle | Mahnoosh Parsaeimehr Ali Misaghi Afshin Akhondzade Ashkan Jebelli Javan Mehdi Taheri Nabi Shariatifar The Effect Of Lactobacillus Paracasei Isolated From Yoghurt On The Growth And Production Of Staphylococcus Aureus Enterotoxin پیاورد سلامت lactobacillus paracasei enterotoxin staphylococcus aureus |
| title | The Effect Of Lactobacillus Paracasei Isolated From Yoghurt On The Growth And Production Of Staphylococcus Aureus Enterotoxin |
| title_full | The Effect Of Lactobacillus Paracasei Isolated From Yoghurt On The Growth And Production Of Staphylococcus Aureus Enterotoxin |
| title_fullStr | The Effect Of Lactobacillus Paracasei Isolated From Yoghurt On The Growth And Production Of Staphylococcus Aureus Enterotoxin |
| title_full_unstemmed | The Effect Of Lactobacillus Paracasei Isolated From Yoghurt On The Growth And Production Of Staphylococcus Aureus Enterotoxin |
| title_short | The Effect Of Lactobacillus Paracasei Isolated From Yoghurt On The Growth And Production Of Staphylococcus Aureus Enterotoxin |
| title_sort | effect of lactobacillus paracasei isolated from yoghurt on the growth and production of staphylococcus aureus enterotoxin |
| topic | lactobacillus paracasei enterotoxin staphylococcus aureus |
| url | http://payavard.tums.ac.ir/browse.php?a_code=A-10-1-75&slc_lang=en&sid=1 |
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