Evaluation of Antibacterial Activity of Two Different Honeys Against Clinical Isolates of β-hemolytic Streptococci Group A
Introduction: Aim of this investigation was to evaluate in vitro antibacterial activity of horse chestnut honey and acacia honey at different concentrations against clinical isolates of β-hemolytic streptococci group A (BHS-A). Honey’s active compounds have multiple therapeutic effects and it is use...
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| Format: | Article |
| Language: | English |
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Faculty of Medicine Osijek
2017-04-01
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| Series: | Southeastern European Medical Journal |
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| Online Access: | http://seemedj.mefos.unios.hr/index.php/seemedj/article/view/seemed17108/12 |
| _version_ | 1818137452691324928 |
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| author | Ivan Kotris Jasminka Talapko Domagoj Drenjančević |
| author_facet | Ivan Kotris Jasminka Talapko Domagoj Drenjančević |
| author_sort | Ivan Kotris |
| collection | DOAJ |
| description | Introduction: Aim of this investigation was to evaluate in vitro antibacterial activity of horse chestnut honey and acacia honey at different concentrations against clinical isolates of β-hemolytic streptococci group A (BHS-A). Honey’s active compounds have multiple therapeutic effects and it is used as traditional medicine for treatment and prevention of various illnesses.
Materials and methods: Antimicrobial effect of two honeys were tested on forty four strains of BHS-A isolated from throat by modified disk diffusion method. A bacterial suspension of BHS-A strains was plated onto Müller-Hinton agar with 5% defibrinated horse blood. Using a sterile 8 mm diameter cork borer, wells were cut in the agar and into each was introduced 100µL of the different concentrations of the honey solution (25 %v/v, 50 %v/v, 75% v/v and 100%v/v). Disk of penicillin was added as the positive control. The plates were incubated aerobically during 18-24 hours at 36 (± 1) °C and zones of inhibition were mesaured.
Results: The average diameter of the inhibition zones of acacia honey (100 %v/v) was 12.48mm±1.73mm, for acacia honey (75 %v/v)was 11.06mm±1.24 mm and for horse chestnut honey (100 %v/v) was 11.08mm±1.02mm. Positive control showed the average diameter of 30.45mm ±3.21mm. Statistically significance (p < 0.05) was observed comparing the zone diameters of the acacia honey (100%) and antibiotic penicillin, and between horse chestnut honey (100%) and antibiotic penicillin.
Conclusion: Acacia and horse chestnut honeys have small, but effective antibacterial activity onto clinical isolates of BHS-A. |
| first_indexed | 2024-12-11T09:56:32Z |
| format | Article |
| id | doaj.art-f93ed82cb9034f6a9760c6a45713a3e7 |
| institution | Directory Open Access Journal |
| issn | 2459-9484 |
| language | English |
| last_indexed | 2024-12-11T09:56:32Z |
| publishDate | 2017-04-01 |
| publisher | Faculty of Medicine Osijek |
| record_format | Article |
| series | Southeastern European Medical Journal |
| spelling | doaj.art-f93ed82cb9034f6a9760c6a45713a3e72022-12-22T01:12:16ZengFaculty of Medicine OsijekSoutheastern European Medical Journal2459-94842017-04-0111677310.26332/seemedj.v1i1.25Evaluation of Antibacterial Activity of Two Different Honeys Against Clinical Isolates of β-hemolytic Streptococci Group AIvan Kotris0Jasminka Talapko1Domagoj Drenjančević2General Hospital Vukovar, Department of Internal Medicine, Vukovar, CroatiaDepartment of Microbiology and Parasitology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia Department of Microbiology and Parasitology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia; University Hospital Center Osijek, Clinical Institute of Transfusion Medicine, Osijek, CroatiaIntroduction: Aim of this investigation was to evaluate in vitro antibacterial activity of horse chestnut honey and acacia honey at different concentrations against clinical isolates of β-hemolytic streptococci group A (BHS-A). Honey’s active compounds have multiple therapeutic effects and it is used as traditional medicine for treatment and prevention of various illnesses. Materials and methods: Antimicrobial effect of two honeys were tested on forty four strains of BHS-A isolated from throat by modified disk diffusion method. A bacterial suspension of BHS-A strains was plated onto Müller-Hinton agar with 5% defibrinated horse blood. Using a sterile 8 mm diameter cork borer, wells were cut in the agar and into each was introduced 100µL of the different concentrations of the honey solution (25 %v/v, 50 %v/v, 75% v/v and 100%v/v). Disk of penicillin was added as the positive control. The plates were incubated aerobically during 18-24 hours at 36 (± 1) °C and zones of inhibition were mesaured. Results: The average diameter of the inhibition zones of acacia honey (100 %v/v) was 12.48mm±1.73mm, for acacia honey (75 %v/v)was 11.06mm±1.24 mm and for horse chestnut honey (100 %v/v) was 11.08mm±1.02mm. Positive control showed the average diameter of 30.45mm ±3.21mm. Statistically significance (p < 0.05) was observed comparing the zone diameters of the acacia honey (100%) and antibiotic penicillin, and between horse chestnut honey (100%) and antibiotic penicillin. Conclusion: Acacia and horse chestnut honeys have small, but effective antibacterial activity onto clinical isolates of BHS-A.http://seemedj.mefos.unios.hr/index.php/seemedj/article/view/seemed17108/12antibacterial activityacacia honeyhorse chestnut honeybeta - hemolytic streptococci group A |
| spellingShingle | Ivan Kotris Jasminka Talapko Domagoj Drenjančević Evaluation of Antibacterial Activity of Two Different Honeys Against Clinical Isolates of β-hemolytic Streptococci Group A Southeastern European Medical Journal antibacterial activity acacia honey horse chestnut honey beta - hemolytic streptococci group A |
| title | Evaluation of Antibacterial Activity of Two Different Honeys Against Clinical Isolates of β-hemolytic Streptococci Group A |
| title_full | Evaluation of Antibacterial Activity of Two Different Honeys Against Clinical Isolates of β-hemolytic Streptococci Group A |
| title_fullStr | Evaluation of Antibacterial Activity of Two Different Honeys Against Clinical Isolates of β-hemolytic Streptococci Group A |
| title_full_unstemmed | Evaluation of Antibacterial Activity of Two Different Honeys Against Clinical Isolates of β-hemolytic Streptococci Group A |
| title_short | Evaluation of Antibacterial Activity of Two Different Honeys Against Clinical Isolates of β-hemolytic Streptococci Group A |
| title_sort | evaluation of antibacterial activity of two different honeys against clinical isolates of β hemolytic streptococci group a |
| topic | antibacterial activity acacia honey horse chestnut honey beta - hemolytic streptococci group A |
| url | http://seemedj.mefos.unios.hr/index.php/seemedj/article/view/seemed17108/12 |
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