Improvement of Enantiomeric <span style="font-variant: small-caps">l</span>-Lactic Acid Production from Mixed Hexose-Pentose Sugars by Coculture of <i>Enterococcus mundtii</i> WX1 and <i>Lactobacillus rhamnosus</i> SCJ9
Among 39 pentose-utilizing lactic acid bacteria (LAB) selected from acid-forming bacteria from the midgut of Eri silkworm, the isolate WX1 was selected with the highest capability to produce optically pure <span style="font-variant: small-caps;">l</span>-lactic acid (<span s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/7/2/95 |
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| author | Augchararat Klongklaew Kridsada Unban Apinun Kanpiengjai Pairote Wongputtisin Punnita Pamueangmun Kalidas Shetty Chartchai Khanongnuch |
| author_facet | Augchararat Klongklaew Kridsada Unban Apinun Kanpiengjai Pairote Wongputtisin Punnita Pamueangmun Kalidas Shetty Chartchai Khanongnuch |
| author_sort | Augchararat Klongklaew |
| collection | DOAJ |
| description | Among 39 pentose-utilizing lactic acid bacteria (LAB) selected from acid-forming bacteria from the midgut of Eri silkworm, the isolate WX1 was selected with the highest capability to produce optically pure <span style="font-variant: small-caps;">l</span>-lactic acid (<span style="font-variant: small-caps;">l</span>-LA) from glucose, xylose and arabinose with furfural-tolerant properties. The isolate WX1 was identified as <i>Enterococcus mundtii</i> based on 16S rDNA sequence analysis. The conversion yields of <span style="font-variant: small-caps;">l</span>-LA from glucose and xylose by <i>E. mundtii</i> WX1 were 0.97 and 0.68 g/g substrate, respectively. Furthermore, <span style="font-variant: small-caps;">l</span>-LA production by <i>E. mundtii</i> WX1 in various glucose-xylose mixtures indicated glucose repression effect on xylose consumption. The coculture of <i>E. mundtii</i> WX1 and <i>Lactobacillus rhamnosus</i> SCJ9, a homofermentative LAB capable of producing <span style="font-variant: small-caps;">l</span>-LA from glucose clearly showed an improvement of <span style="font-variant: small-caps;">l</span>-LA production from 30 g/L total glucose-xylose (6:4). The results from Plackett–Burman design (PBD) indicated that Tween 80, MnSO<sub>4</sub> and yeast extract (YE) were three medium components that significantly influenced (<i>p</i> < 0.05) <span style="font-variant: small-caps;">l</span>-LA production using the coculture strategy in the presence of 2 g/L furfural. Optimal concentrations of these variables revealed by central composite design (CCD) and response surface methodology (RSM) were 20.61 g/L YE, 1.44 g/L Tween 80 and 1.27 g/L MnSO<sub>4</sub>. Based on the optimized medium with 30 g/L total glucose-xylose (6:4), the maximum experimental <span style="font-variant: small-caps;">l</span>-LA value of 23.59 g/L reflecting 0.76 g/g substrate were achieved from 48 h fermentation at 37 °C. <span style="font-variant: small-caps;">l</span>-LA produced by coculture cultivated under standard MRS medium and new optimized conditions were 1.28 and 1.53 times higher than that obtained from single culture by <i>E. mundtii</i> WX1, respectively. This study provides the foundations for practical applications of coculture in bioconversion of lignocellulose particularly glucose-xylose-rich corn stover to <span style="font-variant: small-caps;">l</span>-LA. |
| first_indexed | 2024-03-10T10:32:46Z |
| format | Article |
| id | doaj.art-f9431c0b66b8441fa37644c1406fa7dc |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T10:32:46Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-f9431c0b66b8441fa37644c1406fa7dc2023-11-21T23:35:10ZengMDPI AGFermentation2311-56372021-06-01729510.3390/fermentation7020095Improvement of Enantiomeric <span style="font-variant: small-caps">l</span>-Lactic Acid Production from Mixed Hexose-Pentose Sugars by Coculture of <i>Enterococcus mundtii</i> WX1 and <i>Lactobacillus rhamnosus</i> SCJ9Augchararat Klongklaew0Kridsada Unban1Apinun Kanpiengjai2Pairote Wongputtisin3Punnita Pamueangmun4Kalidas Shetty5Chartchai Khanongnuch6Interdisciplinary Program in Biotechnology, The Graduate School, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Biotechnology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Muang, Chiang Mai 50100, ThailandDivision of Biochemistry and Biochemical Technology, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandProgram in Biotechnology, Faculty of Science, Maejo University, Chiang Mai 50290, ThailandInterdisciplinary Program in Biotechnology, The Graduate School, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Plant Sciences, Global Institute of Food Security and International Agriculture (GIFSIA), North Dakota State University, Fargo, ND 58108, USAInterdisciplinary Program in Biotechnology, The Graduate School, Chiang Mai University, Chiang Mai 50200, ThailandAmong 39 pentose-utilizing lactic acid bacteria (LAB) selected from acid-forming bacteria from the midgut of Eri silkworm, the isolate WX1 was selected with the highest capability to produce optically pure <span style="font-variant: small-caps;">l</span>-lactic acid (<span style="font-variant: small-caps;">l</span>-LA) from glucose, xylose and arabinose with furfural-tolerant properties. The isolate WX1 was identified as <i>Enterococcus mundtii</i> based on 16S rDNA sequence analysis. The conversion yields of <span style="font-variant: small-caps;">l</span>-LA from glucose and xylose by <i>E. mundtii</i> WX1 were 0.97 and 0.68 g/g substrate, respectively. Furthermore, <span style="font-variant: small-caps;">l</span>-LA production by <i>E. mundtii</i> WX1 in various glucose-xylose mixtures indicated glucose repression effect on xylose consumption. The coculture of <i>E. mundtii</i> WX1 and <i>Lactobacillus rhamnosus</i> SCJ9, a homofermentative LAB capable of producing <span style="font-variant: small-caps;">l</span>-LA from glucose clearly showed an improvement of <span style="font-variant: small-caps;">l</span>-LA production from 30 g/L total glucose-xylose (6:4). The results from Plackett–Burman design (PBD) indicated that Tween 80, MnSO<sub>4</sub> and yeast extract (YE) were three medium components that significantly influenced (<i>p</i> < 0.05) <span style="font-variant: small-caps;">l</span>-LA production using the coculture strategy in the presence of 2 g/L furfural. Optimal concentrations of these variables revealed by central composite design (CCD) and response surface methodology (RSM) were 20.61 g/L YE, 1.44 g/L Tween 80 and 1.27 g/L MnSO<sub>4</sub>. Based on the optimized medium with 30 g/L total glucose-xylose (6:4), the maximum experimental <span style="font-variant: small-caps;">l</span>-LA value of 23.59 g/L reflecting 0.76 g/g substrate were achieved from 48 h fermentation at 37 °C. <span style="font-variant: small-caps;">l</span>-LA produced by coculture cultivated under standard MRS medium and new optimized conditions were 1.28 and 1.53 times higher than that obtained from single culture by <i>E. mundtii</i> WX1, respectively. This study provides the foundations for practical applications of coculture in bioconversion of lignocellulose particularly glucose-xylose-rich corn stover to <span style="font-variant: small-caps;">l</span>-LA.https://www.mdpi.com/2311-5637/7/2/95lactic acid bacteriacoculturesilkworm<span style="font-variant: small-caps">l</span>-lactic acidmedium optimization |
| spellingShingle | Augchararat Klongklaew Kridsada Unban Apinun Kanpiengjai Pairote Wongputtisin Punnita Pamueangmun Kalidas Shetty Chartchai Khanongnuch Improvement of Enantiomeric <span style="font-variant: small-caps">l</span>-Lactic Acid Production from Mixed Hexose-Pentose Sugars by Coculture of <i>Enterococcus mundtii</i> WX1 and <i>Lactobacillus rhamnosus</i> SCJ9 Fermentation lactic acid bacteria coculture silkworm <span style="font-variant: small-caps">l</span>-lactic acid medium optimization |
| title | Improvement of Enantiomeric <span style="font-variant: small-caps">l</span>-Lactic Acid Production from Mixed Hexose-Pentose Sugars by Coculture of <i>Enterococcus mundtii</i> WX1 and <i>Lactobacillus rhamnosus</i> SCJ9 |
| title_full | Improvement of Enantiomeric <span style="font-variant: small-caps">l</span>-Lactic Acid Production from Mixed Hexose-Pentose Sugars by Coculture of <i>Enterococcus mundtii</i> WX1 and <i>Lactobacillus rhamnosus</i> SCJ9 |
| title_fullStr | Improvement of Enantiomeric <span style="font-variant: small-caps">l</span>-Lactic Acid Production from Mixed Hexose-Pentose Sugars by Coculture of <i>Enterococcus mundtii</i> WX1 and <i>Lactobacillus rhamnosus</i> SCJ9 |
| title_full_unstemmed | Improvement of Enantiomeric <span style="font-variant: small-caps">l</span>-Lactic Acid Production from Mixed Hexose-Pentose Sugars by Coculture of <i>Enterococcus mundtii</i> WX1 and <i>Lactobacillus rhamnosus</i> SCJ9 |
| title_short | Improvement of Enantiomeric <span style="font-variant: small-caps">l</span>-Lactic Acid Production from Mixed Hexose-Pentose Sugars by Coculture of <i>Enterococcus mundtii</i> WX1 and <i>Lactobacillus rhamnosus</i> SCJ9 |
| title_sort | improvement of enantiomeric span style font variant small caps l span lactic acid production from mixed hexose pentose sugars by coculture of i enterococcus mundtii i wx1 and i lactobacillus rhamnosus i scj9 |
| topic | lactic acid bacteria coculture silkworm <span style="font-variant: small-caps">l</span>-lactic acid medium optimization |
| url | https://www.mdpi.com/2311-5637/7/2/95 |
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