Isolation of Ethyl <i>p</i>-Methoxycinnamate from <i>Azadirachta indica</i> Root Bark as Hong Kong Caterpillar (<i>Tenebrio molitor</i>) Antifeedant
This study aims to identify the antifeedant activity of Azadirachta indica A. Juss root bark against Hong Kong caterpillar (Tenebrio molitor). A. indica A. root was macerated in n-hexane, ethyl acetate, and methanol. The investigation of antifeedant activity was conducted by using the no choice leaf...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Department of Chemistry, Universitas Gadjah Mada
2021-10-01
|
| Series: | Indonesian Journal of Chemistry |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijc/article/view/64487 |
| _version_ | 1828944449297711104 |
|---|---|
| author | Rosnani Nasution Cut Nyak Candein Nurdin Saidi Muhammad Bahi Marianne Marianne |
| author_facet | Rosnani Nasution Cut Nyak Candein Nurdin Saidi Muhammad Bahi Marianne Marianne |
| author_sort | Rosnani Nasution |
| collection | DOAJ |
| description | This study aims to identify the antifeedant activity of Azadirachta indica A. Juss root bark against Hong Kong caterpillar (Tenebrio molitor). A. indica A. root was macerated in n-hexane, ethyl acetate, and methanol. The investigation of antifeedant activity was conducted by using the no choice leaf disk method. The antifeedant activity (%AI) tests showed that the extracts of n-hexane, ethyl acetate, and methanol with each concentration of 0.5% were 6.71, 71.78, and 40.14%, respectively. The ethyl acetate extract demonstrated the most active antifeedant activity. Ethyl acetate extract was fractionated to obtain five subfractions (A-E). Each subfraction with each concentration of 0.5% showed the %AI of 70.55, 85.29; 67.40, 82.70 and 82.22%, respectively. Furthermore, GC-MS results demonstrated that ethyl acetate extract contained 19 compounds. The main compounds are methyl hexadecanoate and methyl 8-octadecenoate. Further purification of fraction A, which has the highest amount, was then carried out. The obtained isolate, i.e., A32, was determined as ethyl p-methoxycinnamate confirmed by 13C- and 1H-NMR, FTIR, and MS spectra. The presence of ethyl p-methoxycinnamate makes A. indica A. root is potential as T. molitor antifeedant. The implication of these findings is to reference herbal antifeedants and reduce the use of synthetic pesticides. |
| first_indexed | 2024-12-14T04:34:05Z |
| format | Article |
| id | doaj.art-f943e9045bf04de2b13d8dac914718f7 |
| institution | Directory Open Access Journal |
| issn | 1411-9420 2460-1578 |
| language | English |
| last_indexed | 2024-12-14T04:34:05Z |
| publishDate | 2021-10-01 |
| publisher | Department of Chemistry, Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Chemistry |
| spelling | doaj.art-f943e9045bf04de2b13d8dac914718f72022-12-21T23:17:00ZengDepartment of Chemistry, Universitas Gadjah MadaIndonesian Journal of Chemistry1411-94202460-15782021-10-012161348135710.22146/ijc.6448730662Isolation of Ethyl <i>p</i>-Methoxycinnamate from <i>Azadirachta indica</i> Root Bark as Hong Kong Caterpillar (<i>Tenebrio molitor</i>) AntifeedantRosnani Nasution0Cut Nyak Candein1Nurdin Saidi2Muhammad Bahi3Marianne Marianne4Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, IndonesiaDepartment of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, IndonesiaThis study aims to identify the antifeedant activity of Azadirachta indica A. Juss root bark against Hong Kong caterpillar (Tenebrio molitor). A. indica A. root was macerated in n-hexane, ethyl acetate, and methanol. The investigation of antifeedant activity was conducted by using the no choice leaf disk method. The antifeedant activity (%AI) tests showed that the extracts of n-hexane, ethyl acetate, and methanol with each concentration of 0.5% were 6.71, 71.78, and 40.14%, respectively. The ethyl acetate extract demonstrated the most active antifeedant activity. Ethyl acetate extract was fractionated to obtain five subfractions (A-E). Each subfraction with each concentration of 0.5% showed the %AI of 70.55, 85.29; 67.40, 82.70 and 82.22%, respectively. Furthermore, GC-MS results demonstrated that ethyl acetate extract contained 19 compounds. The main compounds are methyl hexadecanoate and methyl 8-octadecenoate. Further purification of fraction A, which has the highest amount, was then carried out. The obtained isolate, i.e., A32, was determined as ethyl p-methoxycinnamate confirmed by 13C- and 1H-NMR, FTIR, and MS spectra. The presence of ethyl p-methoxycinnamate makes A. indica A. root is potential as T. molitor antifeedant. The implication of these findings is to reference herbal antifeedants and reduce the use of synthetic pesticides.https://jurnal.ugm.ac.id/ijc/article/view/64487azadirachta indica a. juss root bark, antifeedant, ethyl p-methoxycinnamate, tenebrio molitor. |
| spellingShingle | Rosnani Nasution Cut Nyak Candein Nurdin Saidi Muhammad Bahi Marianne Marianne Isolation of Ethyl <i>p</i>-Methoxycinnamate from <i>Azadirachta indica</i> Root Bark as Hong Kong Caterpillar (<i>Tenebrio molitor</i>) Antifeedant Indonesian Journal of Chemistry azadirachta indica a. juss root bark, antifeedant, ethyl p-methoxycinnamate, tenebrio molitor. |
| title | Isolation of Ethyl <i>p</i>-Methoxycinnamate from <i>Azadirachta indica</i> Root Bark as Hong Kong Caterpillar (<i>Tenebrio molitor</i>) Antifeedant |
| title_full | Isolation of Ethyl <i>p</i>-Methoxycinnamate from <i>Azadirachta indica</i> Root Bark as Hong Kong Caterpillar (<i>Tenebrio molitor</i>) Antifeedant |
| title_fullStr | Isolation of Ethyl <i>p</i>-Methoxycinnamate from <i>Azadirachta indica</i> Root Bark as Hong Kong Caterpillar (<i>Tenebrio molitor</i>) Antifeedant |
| title_full_unstemmed | Isolation of Ethyl <i>p</i>-Methoxycinnamate from <i>Azadirachta indica</i> Root Bark as Hong Kong Caterpillar (<i>Tenebrio molitor</i>) Antifeedant |
| title_short | Isolation of Ethyl <i>p</i>-Methoxycinnamate from <i>Azadirachta indica</i> Root Bark as Hong Kong Caterpillar (<i>Tenebrio molitor</i>) Antifeedant |
| title_sort | isolation of ethyl i p i methoxycinnamate from i azadirachta indica i root bark as hong kong caterpillar i tenebrio molitor i antifeedant |
| topic | azadirachta indica a. juss root bark, antifeedant, ethyl p-methoxycinnamate, tenebrio molitor. |
| url | https://jurnal.ugm.ac.id/ijc/article/view/64487 |
| work_keys_str_mv | AT rosnaninasution isolationofethylipimethoxycinnamatefromiazadirachtaindicairootbarkashongkongcaterpillaritenebriomolitoriantifeedant AT cutnyakcandein isolationofethylipimethoxycinnamatefromiazadirachtaindicairootbarkashongkongcaterpillaritenebriomolitoriantifeedant AT nurdinsaidi isolationofethylipimethoxycinnamatefromiazadirachtaindicairootbarkashongkongcaterpillaritenebriomolitoriantifeedant AT muhammadbahi isolationofethylipimethoxycinnamatefromiazadirachtaindicairootbarkashongkongcaterpillaritenebriomolitoriantifeedant AT mariannemarianne isolationofethylipimethoxycinnamatefromiazadirachtaindicairootbarkashongkongcaterpillaritenebriomolitoriantifeedant |