Serpin7 controls egg diapause of migratory locust (Locusta migratoria) by regulating polyphenol oxidase
Diapause is a state of arrested growth, which allows insects to adapt to diverse environments. Serine protease inhibitors (serpins) play an important role in various physiological processes, including blood coagulation, fibrinolysis, development, complement activation and extracellular matrix remode...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-05-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12825 |
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| author | Jun Chen Dongnan Cui Hidayat Ullah Kun Hao Xiongbing Tu Zehua Zhang |
| author_facet | Jun Chen Dongnan Cui Hidayat Ullah Kun Hao Xiongbing Tu Zehua Zhang |
| author_sort | Jun Chen |
| collection | DOAJ |
| description | Diapause is a state of arrested growth, which allows insects to adapt to diverse environments. Serine protease inhibitors (serpins) play an important role in various physiological processes, including blood coagulation, fibrinolysis, development, complement activation and extracellular matrix remodeling. We hypothesized that serpin may affect energy metabolism and thereby control diapause of migratory locust (Locusta migratoria) embryos by regulating protease cascades. A total of seven nonredundant serpin genes (named serpin1–serpin7) of L. migratoria were obtained through transcriptomic analysis. We further performed label‐free proteomic sequencing and analysis of diapause and nondiapause eggs of L. migratoria, revealing significant differences in serpin7 expression. A significant reduction in diapause rate under the short photoperiod was observed in insects treated with serpin7 double‐stranded RNA. Furthermore, knockdown of the serpin7 gene resulted in significant upregulation of the activity of polyphenol oxidase. We therefore propose that the observed serpin7 gene plays a crucial role in diapause, suggesting that control of energy metabolism may have potential as a future strategy for the reproductive control of insect pests. |
| first_indexed | 2024-12-12T05:10:21Z |
| format | Article |
| id | doaj.art-f949d9298c2a4e9ba78931f1458495a1 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-12-12T05:10:21Z |
| publishDate | 2020-05-01 |
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| spelling | doaj.art-f949d9298c2a4e9ba78931f1458495a12022-12-22T00:36:57ZengWileyFEBS Open Bio2211-54632020-05-0110570771710.1002/2211-5463.12825Serpin7 controls egg diapause of migratory locust (Locusta migratoria) by regulating polyphenol oxidaseJun Chen0Dongnan Cui1Hidayat Ullah2Kun Hao3Xiongbing Tu4Zehua Zhang5State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing ChinaDiapause is a state of arrested growth, which allows insects to adapt to diverse environments. Serine protease inhibitors (serpins) play an important role in various physiological processes, including blood coagulation, fibrinolysis, development, complement activation and extracellular matrix remodeling. We hypothesized that serpin may affect energy metabolism and thereby control diapause of migratory locust (Locusta migratoria) embryos by regulating protease cascades. A total of seven nonredundant serpin genes (named serpin1–serpin7) of L. migratoria were obtained through transcriptomic analysis. We further performed label‐free proteomic sequencing and analysis of diapause and nondiapause eggs of L. migratoria, revealing significant differences in serpin7 expression. A significant reduction in diapause rate under the short photoperiod was observed in insects treated with serpin7 double‐stranded RNA. Furthermore, knockdown of the serpin7 gene resulted in significant upregulation of the activity of polyphenol oxidase. We therefore propose that the observed serpin7 gene plays a crucial role in diapause, suggesting that control of energy metabolism may have potential as a future strategy for the reproductive control of insect pests.https://doi.org/10.1002/2211-5463.12825diapauselocustLocusta migratoriapolyphenol oxidaseRNAiserpin |
| spellingShingle | Jun Chen Dongnan Cui Hidayat Ullah Kun Hao Xiongbing Tu Zehua Zhang Serpin7 controls egg diapause of migratory locust (Locusta migratoria) by regulating polyphenol oxidase FEBS Open Bio diapause locust Locusta migratoria polyphenol oxidase RNAi serpin |
| title | Serpin7 controls egg diapause of migratory locust (Locusta migratoria) by regulating polyphenol oxidase |
| title_full | Serpin7 controls egg diapause of migratory locust (Locusta migratoria) by regulating polyphenol oxidase |
| title_fullStr | Serpin7 controls egg diapause of migratory locust (Locusta migratoria) by regulating polyphenol oxidase |
| title_full_unstemmed | Serpin7 controls egg diapause of migratory locust (Locusta migratoria) by regulating polyphenol oxidase |
| title_short | Serpin7 controls egg diapause of migratory locust (Locusta migratoria) by regulating polyphenol oxidase |
| title_sort | serpin7 controls egg diapause of migratory locust locusta migratoria by regulating polyphenol oxidase |
| topic | diapause locust Locusta migratoria polyphenol oxidase RNAi serpin |
| url | https://doi.org/10.1002/2211-5463.12825 |
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