Towards optimized tissue regeneration: a new 3D printable bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate
Introduction:Autologous platelet concentrate (APC) are pro-angiogenic and can promote wound healing and tissue repair, also in combination with other biomaterials. However, challenging defect situations remain demanding. 3D bioprinting of an APC based bioink encapsulated in a hydrogel could overcome...
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| Format: | Article |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1363380/full |
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| author | Till Grandjean Natarajan Perumal Caroline Manicam Björn Matthey Tao Wu Daniel G. E. Thiem Stefan Stein Dirk Henrich Peer W. Kämmerer Bilal Al-Nawas Ulrike Ritz Ulrike Ritz Sebastian Blatt Sebastian Blatt |
| author_facet | Till Grandjean Natarajan Perumal Caroline Manicam Björn Matthey Tao Wu Daniel G. E. Thiem Stefan Stein Dirk Henrich Peer W. Kämmerer Bilal Al-Nawas Ulrike Ritz Ulrike Ritz Sebastian Blatt Sebastian Blatt |
| author_sort | Till Grandjean |
| collection | DOAJ |
| description | Introduction:Autologous platelet concentrate (APC) are pro-angiogenic and can promote wound healing and tissue repair, also in combination with other biomaterials. However, challenging defect situations remain demanding. 3D bioprinting of an APC based bioink encapsulated in a hydrogel could overcome this limitation with enhanced physio-mechanical interface, growth factor retention/secretion and defect-personalized shape to ultimately enhance regeneration.Methods:This study used extrusion-based bioprinting to create a novel bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate. Chemico-physical testing exhibited an amorphous structure characterized by high shape fidelity. Cytotoxicity assay and incubation of human osteogenic sarcoma cells (SaOs2) exposed excellent biocompatibility. enzyme-linked immunosorbent assay analysis confirmed pro-angiogenic growth factor release of the printed constructs, and co-incubation with HUVECS displayed proper cell viability and proliferation. Chorioallantoic membrane (CAM) assay explored the pro-angiogenic potential of the prints in vivo. Detailed proteome and secretome analysis revealed a substantial amount and homologous presence of pro-angiogenic proteins in the 3D construct.Results:This study demonstrated a 3D bioprinting approach to fabricate a novel bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate with high shape fidelity, biocompatibility, and substantial pro-angiogenic properties.Conclusion:This approach may be suitable for challenging physiological and anatomical defect situations when translated into clinical use. |
| first_indexed | 2024-04-24T19:18:03Z |
| format | Article |
| id | doaj.art-f94a2e03f41a4c73ad8bb71eefec4252 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-24T19:18:03Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-f94a2e03f41a4c73ad8bb71eefec42522024-03-26T04:57:30ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-03-011210.3389/fbioe.2024.13633801363380Towards optimized tissue regeneration: a new 3D printable bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrateTill Grandjean0Natarajan Perumal1Caroline Manicam2Björn Matthey3Tao Wu4Daniel G. E. Thiem5Stefan Stein6Dirk Henrich7Peer W. Kämmerer8Bilal Al-Nawas9Ulrike Ritz10Ulrike Ritz11Sebastian Blatt12Sebastian Blatt13Department of Orthopedics and Traumatology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, GermanyDepartment of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, GermanyDepartment of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, GermanyFraunhofer Institute for Ceramic Technologies and Systems (Fraunhofer IKTS), Dresden, GermanyFraunhofer Institute for Ceramic Technologies and Systems (Fraunhofer IKTS), Dresden, GermanyDepartment of Oral and Maxillofacial Surgery, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, GermanyGeorg-Speyer-Haus, Institute for Tumor Biology and Experimental Therapy, Frankfurt am Main, GermanyDepartment of Trauma, Hand and Reconstructive Surgery, University Hospital, Goethe University Frankfurt, Frankfurt am Main, GermanyDepartment of Oral and Maxillofacial Surgery, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, GermanyDepartment of Oral and Maxillofacial Surgery, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, GermanyDepartment of Orthopedics and Traumatology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, GermanyPlatform for Biomaterial Research, BiomaTiCS Group, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, GermanyDepartment of Oral and Maxillofacial Surgery, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, GermanyPlatform for Biomaterial Research, BiomaTiCS Group, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, GermanyIntroduction:Autologous platelet concentrate (APC) are pro-angiogenic and can promote wound healing and tissue repair, also in combination with other biomaterials. However, challenging defect situations remain demanding. 3D bioprinting of an APC based bioink encapsulated in a hydrogel could overcome this limitation with enhanced physio-mechanical interface, growth factor retention/secretion and defect-personalized shape to ultimately enhance regeneration.Methods:This study used extrusion-based bioprinting to create a novel bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate. Chemico-physical testing exhibited an amorphous structure characterized by high shape fidelity. Cytotoxicity assay and incubation of human osteogenic sarcoma cells (SaOs2) exposed excellent biocompatibility. enzyme-linked immunosorbent assay analysis confirmed pro-angiogenic growth factor release of the printed constructs, and co-incubation with HUVECS displayed proper cell viability and proliferation. Chorioallantoic membrane (CAM) assay explored the pro-angiogenic potential of the prints in vivo. Detailed proteome and secretome analysis revealed a substantial amount and homologous presence of pro-angiogenic proteins in the 3D construct.Results:This study demonstrated a 3D bioprinting approach to fabricate a novel bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate with high shape fidelity, biocompatibility, and substantial pro-angiogenic properties.Conclusion:This approach may be suitable for challenging physiological and anatomical defect situations when translated into clinical use.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1363380/fulladditive manufacturingbioprintingplatelet rich fibrinhydrogelreconstruction |
| spellingShingle | Till Grandjean Natarajan Perumal Caroline Manicam Björn Matthey Tao Wu Daniel G. E. Thiem Stefan Stein Dirk Henrich Peer W. Kämmerer Bilal Al-Nawas Ulrike Ritz Ulrike Ritz Sebastian Blatt Sebastian Blatt Towards optimized tissue regeneration: a new 3D printable bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate Frontiers in Bioengineering and Biotechnology additive manufacturing bioprinting platelet rich fibrin hydrogel reconstruction |
| title | Towards optimized tissue regeneration: a new 3D printable bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate |
| title_full | Towards optimized tissue regeneration: a new 3D printable bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate |
| title_fullStr | Towards optimized tissue regeneration: a new 3D printable bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate |
| title_full_unstemmed | Towards optimized tissue regeneration: a new 3D printable bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate |
| title_short | Towards optimized tissue regeneration: a new 3D printable bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate |
| title_sort | towards optimized tissue regeneration a new 3d printable bioink of alginate cellulose hydrogel loaded with thrombocyte concentrate |
| topic | additive manufacturing bioprinting platelet rich fibrin hydrogel reconstruction |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1363380/full |
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