The Delivery Strategy of Paclitaxel Nanostructured Lipid Carrier Coated with Platelet Membrane
Strategies for the development of anticancer drug delivery systems have undergone a dramatic transformation in the last few decades. Lipid-based drug delivery systems, such as a nanostructured lipid carrier (NLC), are one of the systems emerging to improve the outcomes of tumor treatments. However,...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-06-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/11/6/807 |
| _version_ | 1797724515096068096 |
|---|---|
| author | Ki-Hyun Bang Young-Guk Na Hyun Wook Huh Sung-Joo Hwang Min-Soo Kim Minki Kim Hong-Ki Lee Cheong-Weon Cho |
| author_facet | Ki-Hyun Bang Young-Guk Na Hyun Wook Huh Sung-Joo Hwang Min-Soo Kim Minki Kim Hong-Ki Lee Cheong-Weon Cho |
| author_sort | Ki-Hyun Bang |
| collection | DOAJ |
| description | Strategies for the development of anticancer drug delivery systems have undergone a dramatic transformation in the last few decades. Lipid-based drug delivery systems, such as a nanostructured lipid carrier (NLC), are one of the systems emerging to improve the outcomes of tumor treatments. However, NLC can act as an intruder and cause an immune response. To overcome this limitation, biomimicry technology was introduced to decorate the surface of the nanoparticles with various cell membrane proteins. Here, we designed paclitaxel (PT)-loaded nanostructured lipid carrier (PT-NLC) with platelet (PLT) membrane protein because PLT is involved with angiogenesis and interaction of circulating tumor cells. After PLT was isolated from blood using the gravity-gradient method and it was used for coating PT-NLC. Spherical PT-NLC and platelet membrane coated PT-NLC (P-PT-NLC) were successfully fabricated with high encapsulation efficiency (EE) (99.98%) and small particle size (less than 200 nm). The successful coating of PT-NLC with a PLT membrane was confirmed by the identification of CD41 based on transmission electron microscopy (TEM), western blot assay and enzyme-linked immunosorbent assay (ELISA) data. Moreover, the stronger affinity of P-PT-NLC than that of PT-NLC toward tumor cells was observed. In vitro cell study, the PLT coated nanoparticles successfully displayed the anti-tumor effect to SK-OV-3 cells. In summary, the biomimicry carrier system P-PT-NLC has an affinity and targeting ability for tumor cells. |
| first_indexed | 2024-03-12T10:18:16Z |
| format | Article |
| id | doaj.art-f94b9bcb166040319274f34e3267df09 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T10:18:16Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-f94b9bcb166040319274f34e3267df092023-09-02T10:23:20ZengMDPI AGCancers2072-66942019-06-0111680710.3390/cancers11060807cancers11060807The Delivery Strategy of Paclitaxel Nanostructured Lipid Carrier Coated with Platelet MembraneKi-Hyun Bang0Young-Guk Na1Hyun Wook Huh2Sung-Joo Hwang3Min-Soo Kim4Minki Kim5Hong-Ki Lee6Cheong-Weon Cho7College of Pharmacy, Chungnam National University, Daejeon 34134, KoreaCollege of Pharmacy, Chungnam National University, Daejeon 34134, KoreaCollege of Pharmacy, Chungnam National University, Daejeon 34134, KoreaCollege of Pharmacy and Yonsei Institute of Pharmaceutical Sciences, Yonsei University, 162-1 Songdo-dong, Yeonsu-gu, Incheon 406-840, KoreaCollege of Pharmacy, Pusan National University, 63 Busandaehak-ro, Geumjeong-gu, Busan 609-735, KoreaCollege of Pharmacy, Chungnam National University, Daejeon 34134, KoreaCollege of Pharmacy, Chungnam National University, Daejeon 34134, KoreaCollege of Pharmacy, Chungnam National University, Daejeon 34134, KoreaStrategies for the development of anticancer drug delivery systems have undergone a dramatic transformation in the last few decades. Lipid-based drug delivery systems, such as a nanostructured lipid carrier (NLC), are one of the systems emerging to improve the outcomes of tumor treatments. However, NLC can act as an intruder and cause an immune response. To overcome this limitation, biomimicry technology was introduced to decorate the surface of the nanoparticles with various cell membrane proteins. Here, we designed paclitaxel (PT)-loaded nanostructured lipid carrier (PT-NLC) with platelet (PLT) membrane protein because PLT is involved with angiogenesis and interaction of circulating tumor cells. After PLT was isolated from blood using the gravity-gradient method and it was used for coating PT-NLC. Spherical PT-NLC and platelet membrane coated PT-NLC (P-PT-NLC) were successfully fabricated with high encapsulation efficiency (EE) (99.98%) and small particle size (less than 200 nm). The successful coating of PT-NLC with a PLT membrane was confirmed by the identification of CD41 based on transmission electron microscopy (TEM), western blot assay and enzyme-linked immunosorbent assay (ELISA) data. Moreover, the stronger affinity of P-PT-NLC than that of PT-NLC toward tumor cells was observed. In vitro cell study, the PLT coated nanoparticles successfully displayed the anti-tumor effect to SK-OV-3 cells. In summary, the biomimicry carrier system P-PT-NLC has an affinity and targeting ability for tumor cells.https://www.mdpi.com/2072-6694/11/6/807nanostructured lipid carrierplatelet membranebiomimicrypaclitaxel |
| spellingShingle | Ki-Hyun Bang Young-Guk Na Hyun Wook Huh Sung-Joo Hwang Min-Soo Kim Minki Kim Hong-Ki Lee Cheong-Weon Cho The Delivery Strategy of Paclitaxel Nanostructured Lipid Carrier Coated with Platelet Membrane Cancers nanostructured lipid carrier platelet membrane biomimicry paclitaxel |
| title | The Delivery Strategy of Paclitaxel Nanostructured Lipid Carrier Coated with Platelet Membrane |
| title_full | The Delivery Strategy of Paclitaxel Nanostructured Lipid Carrier Coated with Platelet Membrane |
| title_fullStr | The Delivery Strategy of Paclitaxel Nanostructured Lipid Carrier Coated with Platelet Membrane |
| title_full_unstemmed | The Delivery Strategy of Paclitaxel Nanostructured Lipid Carrier Coated with Platelet Membrane |
| title_short | The Delivery Strategy of Paclitaxel Nanostructured Lipid Carrier Coated with Platelet Membrane |
| title_sort | delivery strategy of paclitaxel nanostructured lipid carrier coated with platelet membrane |
| topic | nanostructured lipid carrier platelet membrane biomimicry paclitaxel |
| url | https://www.mdpi.com/2072-6694/11/6/807 |
| work_keys_str_mv | AT kihyunbang thedeliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane AT younggukna thedeliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane AT hyunwookhuh thedeliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane AT sungjoohwang thedeliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane AT minsookim thedeliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane AT minkikim thedeliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane AT hongkilee thedeliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane AT cheongweoncho thedeliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane AT kihyunbang deliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane AT younggukna deliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane AT hyunwookhuh deliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane AT sungjoohwang deliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane AT minsookim deliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane AT minkikim deliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane AT hongkilee deliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane AT cheongweoncho deliverystrategyofpaclitaxelnanostructuredlipidcarriercoatedwithplateletmembrane |