Antifungal Effect of Autochthonous Aromatic Plant Extracts on Two Mycotoxigenic Strains of <i>Aspergillus flavus</i>
This study identified the compounds obtained from four native Dehesa plants, which were holm oak, elm, blackberry and white rockrose, and evaluated their ability to inhibit the growth and production of aflatoxins B<sub>1</sub> and B<sub>2</sub> of two strains of mycotoxigenic...
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MDPI AG
2023-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/9/1821 |
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| author | Francisco Ramiro Boy Rocío Casquete Iris Gudiño Almudena V. Merchán Belén Peromingo María José Benito |
| author_facet | Francisco Ramiro Boy Rocío Casquete Iris Gudiño Almudena V. Merchán Belén Peromingo María José Benito |
| author_sort | Francisco Ramiro Boy |
| collection | DOAJ |
| description | This study identified the compounds obtained from four native Dehesa plants, which were holm oak, elm, blackberry and white rockrose, and evaluated their ability to inhibit the growth and production of aflatoxins B<sub>1</sub> and B<sub>2</sub> of two strains of mycotoxigenic <i>Aspergillus flavus</i>. For this purpose, phenolic compounds present in the leaves and flowers of the plants were extracted and identified, and subsequently, the effect on the growth of <i>A. flavus</i>, aflatoxin production and the expression of a gene related to its synthesis were studied. <i>Cistus albidus</i> was the plant with the highest concentration of phenolic compounds, followed by <i>Quercus ilex</i>. Phenolic acids and flavonoids were mainly identified, and there was great variability among plant extracts in terms of the type and quantity of compounds. Concentrated and diluted extracts were used for each individual plant. The influence on mold growth was not very significant for any of the extracts. However, those obtained from plants of the genus <i>Quercus ilex</i>, followed by <i>Ulmus</i> sp., were very useful for inhibiting the production of aflatoxin B<sub>1</sub> and B<sub>2</sub> produced by the two strains of <i>A. flavus</i>. Expression studies of the gene involved in the aflatoxin synthesis pathway did not prove to be effective. The results indicated that using these new natural antifungal compounds from the Dehesa for aflatoxin production inhibition would be desirable, promoting respect for the environment by avoiding the use of chemical fungicides. However, further studies are needed to determine whether the specific phenolic compounds responsible for the antifungal activity of <i>Quercus ilex</i> and <i>Ulmus</i> sp. produce the antifungal activity in pure form, as well as to verify the action mechanism of these compounds. |
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| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T04:19:45Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Foods |
| spelling | doaj.art-f94f26ee9b074579927045277cfbde962023-11-17T22:55:18ZengMDPI AGFoods2304-81582023-04-01129182110.3390/foods12091821Antifungal Effect of Autochthonous Aromatic Plant Extracts on Two Mycotoxigenic Strains of <i>Aspergillus flavus</i>Francisco Ramiro Boy0Rocío Casquete1Iris Gudiño2Almudena V. Merchán3Belén Peromingo4María José Benito5Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Avd. Adolfo Suárez s/n, 06007 Badajoz, SpainNutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Avd. Adolfo Suárez s/n, 06007 Badajoz, SpainNutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Avd. Adolfo Suárez s/n, 06007 Badajoz, SpainNutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Avd. Adolfo Suárez s/n, 06007 Badajoz, SpainNutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Avd. Adolfo Suárez s/n, 06007 Badajoz, SpainNutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Avd. Adolfo Suárez s/n, 06007 Badajoz, SpainThis study identified the compounds obtained from four native Dehesa plants, which were holm oak, elm, blackberry and white rockrose, and evaluated their ability to inhibit the growth and production of aflatoxins B<sub>1</sub> and B<sub>2</sub> of two strains of mycotoxigenic <i>Aspergillus flavus</i>. For this purpose, phenolic compounds present in the leaves and flowers of the plants were extracted and identified, and subsequently, the effect on the growth of <i>A. flavus</i>, aflatoxin production and the expression of a gene related to its synthesis were studied. <i>Cistus albidus</i> was the plant with the highest concentration of phenolic compounds, followed by <i>Quercus ilex</i>. Phenolic acids and flavonoids were mainly identified, and there was great variability among plant extracts in terms of the type and quantity of compounds. Concentrated and diluted extracts were used for each individual plant. The influence on mold growth was not very significant for any of the extracts. However, those obtained from plants of the genus <i>Quercus ilex</i>, followed by <i>Ulmus</i> sp., were very useful for inhibiting the production of aflatoxin B<sub>1</sub> and B<sub>2</sub> produced by the two strains of <i>A. flavus</i>. Expression studies of the gene involved in the aflatoxin synthesis pathway did not prove to be effective. The results indicated that using these new natural antifungal compounds from the Dehesa for aflatoxin production inhibition would be desirable, promoting respect for the environment by avoiding the use of chemical fungicides. However, further studies are needed to determine whether the specific phenolic compounds responsible for the antifungal activity of <i>Quercus ilex</i> and <i>Ulmus</i> sp. produce the antifungal activity in pure form, as well as to verify the action mechanism of these compounds.https://www.mdpi.com/2304-8158/12/9/1821aromatic Dehesa plantsphenolic compoundsantifungal activity<i>Aspergillus flavus</i>aflatoxins |
| spellingShingle | Francisco Ramiro Boy Rocío Casquete Iris Gudiño Almudena V. Merchán Belén Peromingo María José Benito Antifungal Effect of Autochthonous Aromatic Plant Extracts on Two Mycotoxigenic Strains of <i>Aspergillus flavus</i> Foods aromatic Dehesa plants phenolic compounds antifungal activity <i>Aspergillus flavus</i> aflatoxins |
| title | Antifungal Effect of Autochthonous Aromatic Plant Extracts on Two Mycotoxigenic Strains of <i>Aspergillus flavus</i> |
| title_full | Antifungal Effect of Autochthonous Aromatic Plant Extracts on Two Mycotoxigenic Strains of <i>Aspergillus flavus</i> |
| title_fullStr | Antifungal Effect of Autochthonous Aromatic Plant Extracts on Two Mycotoxigenic Strains of <i>Aspergillus flavus</i> |
| title_full_unstemmed | Antifungal Effect of Autochthonous Aromatic Plant Extracts on Two Mycotoxigenic Strains of <i>Aspergillus flavus</i> |
| title_short | Antifungal Effect of Autochthonous Aromatic Plant Extracts on Two Mycotoxigenic Strains of <i>Aspergillus flavus</i> |
| title_sort | antifungal effect of autochthonous aromatic plant extracts on two mycotoxigenic strains of i aspergillus flavus i |
| topic | aromatic Dehesa plants phenolic compounds antifungal activity <i>Aspergillus flavus</i> aflatoxins |
| url | https://www.mdpi.com/2304-8158/12/9/1821 |
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