Imazalil resulted in glucolipid metabolism disturbance and abnormal m6A RNA methylation in the liver of dam and offspring mice
As a fungicide with the characteristics of high effectiveness, internal absorption and broad spectrum, imazalil is widely used to prevent and treat in fruits and vegetables. Here, pregnant C57BL/6 mice were exposed to imazalil at dietary levels of 0, 0.025‰, and 0.25‰ through drinking water during p...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324000381 |
| _version_ | 1797328884549550080 |
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| author | Cuiyuan Jin Caiyun Wang Chen Zhang Qi Ye Yuanxiang Jin Liyun Shi |
| author_facet | Cuiyuan Jin Caiyun Wang Chen Zhang Qi Ye Yuanxiang Jin Liyun Shi |
| author_sort | Cuiyuan Jin |
| collection | DOAJ |
| description | As a fungicide with the characteristics of high effectiveness, internal absorption and broad spectrum, imazalil is widely used to prevent and treat in fruits and vegetables. Here, pregnant C57BL/6 mice were exposed to imazalil at dietary levels of 0, 0.025‰, and 0.25‰ through drinking water during pregnancy and lactation. We then analyzed the phenotype, metabolome, and expression of related genes and proteins in the livers of mice. There was a marked decrease in the body and liver weights of male offspring mice after maternal imazalil exposure, while this effect on the dam and female offspring was slight. Metabolomics analyses revealed that imazalil significantly altered the metabolite composition of liver samples from both dams and offspring. The preliminary results of the analysis indicated that glucolipid metabolism was the pathway most significantly affected by imazalil. We performed a coabundance association analysis of metabolites with significant changes in the pathway of glycolipid metabolism, and IMZ altered the networks of both dams and offspring compared with the network in control mice, especially in male offspring. The hepatic triglyceride, non-esterified fatty acid and glucose levels were increased significantly in the dams but decreased significantly in male offspring after maternal imazalil exposure. Furthermore, the expression levels of genes associated with glycolipid metabolism and m6A RNA methylation were significantly affected by maternal intake of imazalil. Imazalil-induced glucolipid metabolism disturbance was highly correlated with m6A RNA methylation. In conclusion, maternal imazalil exposure resulted in glucolipid metabolism disturbance and abnormal m6A RNA methylation in the livers of dams and offspring mice. We expected that the information acquired in this study will provide novel evidence for understanding the effect of maternal imazalil exposure on potential health risks. |
| first_indexed | 2024-03-08T06:57:26Z |
| format | Article |
| id | doaj.art-f950a0eea47140da964a35e848fbc5eb |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-08T06:57:26Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-f950a0eea47140da964a35e848fbc5eb2024-02-03T06:34:27ZengElsevierEcotoxicology and Environmental Safety0147-65132024-02-01271115963Imazalil resulted in glucolipid metabolism disturbance and abnormal m6A RNA methylation in the liver of dam and offspring miceCuiyuan Jin0Caiyun Wang1Chen Zhang2Qi Ye3Yuanxiang Jin4Liyun Shi5Institute of Translational Medicine, Zhejiang Shuren University, Hangzhou, Zhejiang 310015, ChinaDepartment of Public Health and Preventive Medicine, Changzhi Medical College, Changzhi, Shanxi, 046000, ChinaInstitute of Translational Medicine, Zhejiang Shuren University, Hangzhou, Zhejiang 310015, ChinaInstitute of Translational Medicine, Zhejiang Shuren University, Hangzhou, Zhejiang 310015, ChinaCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310032, China; Correspondence to: 18, Chaowang Road, Hangzhou, Zhejiang Province 310032, China.Institute of Translational Medicine, Zhejiang Shuren University, Hangzhou, Zhejiang 310015, China; Correspondence to: 8, Shuren Road, Hangzhou, Zhejiang Province 310015, China.As a fungicide with the characteristics of high effectiveness, internal absorption and broad spectrum, imazalil is widely used to prevent and treat in fruits and vegetables. Here, pregnant C57BL/6 mice were exposed to imazalil at dietary levels of 0, 0.025‰, and 0.25‰ through drinking water during pregnancy and lactation. We then analyzed the phenotype, metabolome, and expression of related genes and proteins in the livers of mice. There was a marked decrease in the body and liver weights of male offspring mice after maternal imazalil exposure, while this effect on the dam and female offspring was slight. Metabolomics analyses revealed that imazalil significantly altered the metabolite composition of liver samples from both dams and offspring. The preliminary results of the analysis indicated that glucolipid metabolism was the pathway most significantly affected by imazalil. We performed a coabundance association analysis of metabolites with significant changes in the pathway of glycolipid metabolism, and IMZ altered the networks of both dams and offspring compared with the network in control mice, especially in male offspring. The hepatic triglyceride, non-esterified fatty acid and glucose levels were increased significantly in the dams but decreased significantly in male offspring after maternal imazalil exposure. Furthermore, the expression levels of genes associated with glycolipid metabolism and m6A RNA methylation were significantly affected by maternal intake of imazalil. Imazalil-induced glucolipid metabolism disturbance was highly correlated with m6A RNA methylation. In conclusion, maternal imazalil exposure resulted in glucolipid metabolism disturbance and abnormal m6A RNA methylation in the livers of dams and offspring mice. We expected that the information acquired in this study will provide novel evidence for understanding the effect of maternal imazalil exposure on potential health risks.http://www.sciencedirect.com/science/article/pii/S0147651324000381ImazalilGlucolipid metabolismm6A RNA methylationOffspring |
| spellingShingle | Cuiyuan Jin Caiyun Wang Chen Zhang Qi Ye Yuanxiang Jin Liyun Shi Imazalil resulted in glucolipid metabolism disturbance and abnormal m6A RNA methylation in the liver of dam and offspring mice Ecotoxicology and Environmental Safety Imazalil Glucolipid metabolism m6A RNA methylation Offspring |
| title | Imazalil resulted in glucolipid metabolism disturbance and abnormal m6A RNA methylation in the liver of dam and offspring mice |
| title_full | Imazalil resulted in glucolipid metabolism disturbance and abnormal m6A RNA methylation in the liver of dam and offspring mice |
| title_fullStr | Imazalil resulted in glucolipid metabolism disturbance and abnormal m6A RNA methylation in the liver of dam and offspring mice |
| title_full_unstemmed | Imazalil resulted in glucolipid metabolism disturbance and abnormal m6A RNA methylation in the liver of dam and offspring mice |
| title_short | Imazalil resulted in glucolipid metabolism disturbance and abnormal m6A RNA methylation in the liver of dam and offspring mice |
| title_sort | imazalil resulted in glucolipid metabolism disturbance and abnormal m6a rna methylation in the liver of dam and offspring mice |
| topic | Imazalil Glucolipid metabolism m6A RNA methylation Offspring |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324000381 |
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