Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses
Striosomes were discovered several decades ago as neurochemically identified zones in the striatum, yet technical hurdles have hampered the study of the functions of these striatal compartments. Here we used 2-photon calcium imaging in neuronal birthdate-labeled Mash1-CreER;Ai14 mice to image simult...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-12-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/32353 |
| _version_ | 1811181065455271936 |
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| author | Bernard Bloem Rafiq Huda Mriganka Sur Ann M Graybiel |
| author_facet | Bernard Bloem Rafiq Huda Mriganka Sur Ann M Graybiel |
| author_sort | Bernard Bloem |
| collection | DOAJ |
| description | Striosomes were discovered several decades ago as neurochemically identified zones in the striatum, yet technical hurdles have hampered the study of the functions of these striatal compartments. Here we used 2-photon calcium imaging in neuronal birthdate-labeled Mash1-CreER;Ai14 mice to image simultaneously the activity of striosomal and matrix neurons as mice performed an auditory conditioning task. With this method, we identified circumscribed zones of tdTomato-labeled neuropil that correspond to striosomes as verified immunohistochemically. Neurons in both striosomes and matrix responded to reward-predicting cues and were active during or after consummatory licking. However, we found quantitative differences in response strength: striosomal neurons fired more to reward-predicting cues and encoded more information about expected outcome as mice learned the task, whereas matrix neurons were more strongly modulated by recent reward history. These findings open the possibility of harnessing in vivo imaging to determine the contributions of striosomes and matrix to striatal circuit function. |
| first_indexed | 2024-04-11T09:12:16Z |
| format | Article |
| id | doaj.art-f950cb3b2064474098ab4bb8cd7bf103 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:12:16Z |
| publishDate | 2017-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-f950cb3b2064474098ab4bb8cd7bf1032022-12-22T04:32:28ZengeLife Sciences Publications LtdeLife2050-084X2017-12-01610.7554/eLife.32353Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responsesBernard Bloem0https://orcid.org/0000-0002-0930-580XRafiq Huda1Mriganka Sur2Ann M Graybiel3https://orcid.org/0000-0002-4326-7720McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, United States; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, United StatesDepartment of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, United States; Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, United StatesDepartment of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, United States; Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, United StatesMcGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, United States; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, United StatesStriosomes were discovered several decades ago as neurochemically identified zones in the striatum, yet technical hurdles have hampered the study of the functions of these striatal compartments. Here we used 2-photon calcium imaging in neuronal birthdate-labeled Mash1-CreER;Ai14 mice to image simultaneously the activity of striosomal and matrix neurons as mice performed an auditory conditioning task. With this method, we identified circumscribed zones of tdTomato-labeled neuropil that correspond to striosomes as verified immunohistochemically. Neurons in both striosomes and matrix responded to reward-predicting cues and were active during or after consummatory licking. However, we found quantitative differences in response strength: striosomal neurons fired more to reward-predicting cues and encoded more information about expected outcome as mice learned the task, whereas matrix neurons were more strongly modulated by recent reward history. These findings open the possibility of harnessing in vivo imaging to determine the contributions of striosomes and matrix to striatal circuit function.https://elifesciences.org/articles/32353striosomestriatum2-photon imagingreinforcement learningbasal ganglia |
| spellingShingle | Bernard Bloem Rafiq Huda Mriganka Sur Ann M Graybiel Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses eLife striosome striatum 2-photon imaging reinforcement learning basal ganglia |
| title | Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses |
| title_full | Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses |
| title_fullStr | Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses |
| title_full_unstemmed | Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses |
| title_short | Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses |
| title_sort | two photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement related responses |
| topic | striosome striatum 2-photon imaging reinforcement learning basal ganglia |
| url | https://elifesciences.org/articles/32353 |
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