The Efficacy of N-acetylcysteine Against Renal Oxidative Stress After Extracorporeal Shock Wave Treatment: An Experimental Rat Model
Objective:To evaluate effects of renal extracorporeal shock wave lithotripsy (SWL) on plasma Oxidative Stress index (OSI) and to observe histopathological alterations in an experimental model. Secondly, protective role of N-acetylcysteine (NAC) was investigated.Materials and Methods:A total of 24 ra...
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| Format: | Article |
| Language: | English |
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Galenos Yayinevi
2020-03-01
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| Series: | Journal of Urological Surgery |
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http://jurolsurgery.org/archives/archive-detail/article-preview/the-efficacy-of-n-acetylcysteine-against-renal-oxi/36091
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| _version_ | 1828028844257312768 |
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| author | Dursun Baba Kamil Çam Yusuf Şenoğlu Alpaslan Yüksel Havva Erdem Ekrem Başaran |
| author_facet | Dursun Baba Kamil Çam Yusuf Şenoğlu Alpaslan Yüksel Havva Erdem Ekrem Başaran |
| author_sort | Dursun Baba |
| collection | DOAJ |
| description | Objective:To evaluate effects of renal extracorporeal shock wave lithotripsy (SWL) on plasma Oxidative Stress index (OSI) and to observe histopathological alterations in an experimental model. Secondly, protective role of N-acetylcysteine (NAC) was investigated.Materials and Methods:A total of 24 rats were randomly divided into 3 groups as control (group 1), SWL + saline (group 2), and SWL + NAC (group 3). Study groups were further divided into two subgroups as short-term and long-term. In groups 2 and 3, 2000 shock waves were applied. Intraperitoneal saline was administered in group 2, and intraperitoneal NAC was given to group 3. No treatment was administered to group 1. Blood samples and nephrectomy specimens were obtained for biochemical and histopathological examinations, respectively. OSI was calculated by measuring plasma total antioxidant status (TAS) and total oxidant status (TOS). Acute and chronic histopathological damage were evaluated by light microscopy.Results:SWL caused a remarkable increase in oxidative stress. Strikingly, TOS levels were significantly lower (p=0.027) and TAS levels were significantly higher (p=0.006) in rats with SWL + NAC (group 3). As a result, OSI was lower (p=0.013). This effect was particularly significant in the short-term subgroup. It was also concluded that tubular damage and interstitial inflammation were higher in the SWL group (p=0.022). These acute histological alterations were slighter in rats with NAC.Conclusion:The current study demonstrated that SWL can cause severe oxidative stress and acute renal damage by increasing free oxygen radical production. NAC was effective in decreasing SWL-induced oxidative stress and preventing certain histological alterations to some extent. |
| first_indexed | 2024-04-10T14:00:37Z |
| format | Article |
| id | doaj.art-f951782a444443e2a82fa2e696794e23 |
| institution | Directory Open Access Journal |
| issn | 2148-9580 2148-9580 |
| language | English |
| last_indexed | 2024-04-10T14:00:37Z |
| publishDate | 2020-03-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Journal of Urological Surgery |
| spelling | doaj.art-f951782a444443e2a82fa2e696794e232023-02-15T16:10:18ZengGalenos YayineviJournal of Urological Surgery2148-95802148-95802020-03-017181510.4274/jus.galenos.2019.294113049054The Efficacy of N-acetylcysteine Against Renal Oxidative Stress After Extracorporeal Shock Wave Treatment: An Experimental Rat ModelDursun Baba0Kamil Çam1Yusuf Şenoğlu2Alpaslan Yüksel3Havva Erdem4Ekrem Başaran5 Atatürk State Hospital, Clinic of Urology, Düzce, Turkiye Marmara University Faculty of Medicine, Department of Urology, İstanbul, Turkiye Düzce University Faculty of Medicine, Department of Urology, Düzce, Turkiye Düzce University Faculty of Medicine, Department of Urology, Düzce, Turkiye Ordu University Faculty of Medicine, Department of Pathology, Ordu, Turkiye Atatürk State Hospital, Clinic of Urology, Düzce, Turkiye Objective:To evaluate effects of renal extracorporeal shock wave lithotripsy (SWL) on plasma Oxidative Stress index (OSI) and to observe histopathological alterations in an experimental model. Secondly, protective role of N-acetylcysteine (NAC) was investigated.Materials and Methods:A total of 24 rats were randomly divided into 3 groups as control (group 1), SWL + saline (group 2), and SWL + NAC (group 3). Study groups were further divided into two subgroups as short-term and long-term. In groups 2 and 3, 2000 shock waves were applied. Intraperitoneal saline was administered in group 2, and intraperitoneal NAC was given to group 3. No treatment was administered to group 1. Blood samples and nephrectomy specimens were obtained for biochemical and histopathological examinations, respectively. OSI was calculated by measuring plasma total antioxidant status (TAS) and total oxidant status (TOS). Acute and chronic histopathological damage were evaluated by light microscopy.Results:SWL caused a remarkable increase in oxidative stress. Strikingly, TOS levels were significantly lower (p=0.027) and TAS levels were significantly higher (p=0.006) in rats with SWL + NAC (group 3). As a result, OSI was lower (p=0.013). This effect was particularly significant in the short-term subgroup. It was also concluded that tubular damage and interstitial inflammation were higher in the SWL group (p=0.022). These acute histological alterations were slighter in rats with NAC.Conclusion:The current study demonstrated that SWL can cause severe oxidative stress and acute renal damage by increasing free oxygen radical production. NAC was effective in decreasing SWL-induced oxidative stress and preventing certain histological alterations to some extent. http://jurolsurgery.org/archives/archive-detail/article-preview/the-efficacy-of-n-acetylcysteine-against-renal-oxi/36091 extracorporeal shock wave lithotripsykidneyoxidative stressrat |
| spellingShingle | Dursun Baba Kamil Çam Yusuf Şenoğlu Alpaslan Yüksel Havva Erdem Ekrem Başaran The Efficacy of N-acetylcysteine Against Renal Oxidative Stress After Extracorporeal Shock Wave Treatment: An Experimental Rat Model Journal of Urological Surgery extracorporeal shock wave lithotripsy kidney oxidative stress rat |
| title | The Efficacy of N-acetylcysteine Against Renal Oxidative Stress After Extracorporeal Shock Wave Treatment: An Experimental Rat Model |
| title_full | The Efficacy of N-acetylcysteine Against Renal Oxidative Stress After Extracorporeal Shock Wave Treatment: An Experimental Rat Model |
| title_fullStr | The Efficacy of N-acetylcysteine Against Renal Oxidative Stress After Extracorporeal Shock Wave Treatment: An Experimental Rat Model |
| title_full_unstemmed | The Efficacy of N-acetylcysteine Against Renal Oxidative Stress After Extracorporeal Shock Wave Treatment: An Experimental Rat Model |
| title_short | The Efficacy of N-acetylcysteine Against Renal Oxidative Stress After Extracorporeal Shock Wave Treatment: An Experimental Rat Model |
| title_sort | efficacy of n acetylcysteine against renal oxidative stress after extracorporeal shock wave treatment an experimental rat model |
| topic | extracorporeal shock wave lithotripsy kidney oxidative stress rat |
| url |
http://jurolsurgery.org/archives/archive-detail/article-preview/the-efficacy-of-n-acetylcysteine-against-renal-oxi/36091
|
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