Fabrication of alginate-based hydrogel cross-linked via horseradish peroxidase for articular cartilage engineering
Abstract Objective We aimed to detect the effect of a couple of parameters including Alg, H2O2, and HRP concentrations on the gelation time of Alg-based hydrogels using an enzymatic cross-linked procedure. Results NMR, UV–Vis, and ATR-FTIR analyses confirmed the conjugation of Ph to the Alg backbone...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-09-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13104-021-05795-2 |
| _version_ | 1819020911801532416 |
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| author | Sepideh Saghati Ali Baradar Khoshfetrat Hamid Tayefi Nasrabadi Leila Roshangar Reza Rahbarghazi |
| author_facet | Sepideh Saghati Ali Baradar Khoshfetrat Hamid Tayefi Nasrabadi Leila Roshangar Reza Rahbarghazi |
| author_sort | Sepideh Saghati |
| collection | DOAJ |
| description | Abstract Objective We aimed to detect the effect of a couple of parameters including Alg, H2O2, and HRP concentrations on the gelation time of Alg-based hydrogels using an enzymatic cross-linked procedure. Results NMR, UV–Vis, and ATR-FTIR analyses confirmed the conjugation of Ph to the Alg backbone. Data showed gelation time was delayed with the increase and reduction of H2O2 and HRP, respectively. We noted that hydrogel consisted of 1.2% (w/v) Alg, 5 U HRP, and 100 mM H2O2 yielded an appropriate gelation time with appropriate mechanical properties. The addition of 0.5% (v/v) Col developed hydrogel increased the gelation time. The data showed that Alg, HRP, and H2O2 with the ratio of 1:0.54:0.54 had proper physicochemical features for cartilage engineering. |
| first_indexed | 2024-12-21T03:58:44Z |
| format | Article |
| id | doaj.art-f9567174deb941168af1da7ef0e3737d |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-21T03:58:44Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-f9567174deb941168af1da7ef0e3737d2022-12-21T19:16:46ZengBMCBMC Research Notes1756-05002021-09-011411710.1186/s13104-021-05795-2Fabrication of alginate-based hydrogel cross-linked via horseradish peroxidase for articular cartilage engineeringSepideh Saghati0Ali Baradar Khoshfetrat1Hamid Tayefi Nasrabadi2Leila Roshangar3Reza Rahbarghazi4Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical SciencesChemical Engineering Faculty, Sahand University of TechnologyDepartment of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical SciencesDepartment of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical SciencesStem Cell Research Center, Tabriz University of Medical SciencesAbstract Objective We aimed to detect the effect of a couple of parameters including Alg, H2O2, and HRP concentrations on the gelation time of Alg-based hydrogels using an enzymatic cross-linked procedure. Results NMR, UV–Vis, and ATR-FTIR analyses confirmed the conjugation of Ph to the Alg backbone. Data showed gelation time was delayed with the increase and reduction of H2O2 and HRP, respectively. We noted that hydrogel consisted of 1.2% (w/v) Alg, 5 U HRP, and 100 mM H2O2 yielded an appropriate gelation time with appropriate mechanical properties. The addition of 0.5% (v/v) Col developed hydrogel increased the gelation time. The data showed that Alg, HRP, and H2O2 with the ratio of 1:0.54:0.54 had proper physicochemical features for cartilage engineering.https://doi.org/10.1186/s13104-021-05795-2AlginateHydrogelPhenolationEnzymatic cross-linking |
| spellingShingle | Sepideh Saghati Ali Baradar Khoshfetrat Hamid Tayefi Nasrabadi Leila Roshangar Reza Rahbarghazi Fabrication of alginate-based hydrogel cross-linked via horseradish peroxidase for articular cartilage engineering BMC Research Notes Alginate Hydrogel Phenolation Enzymatic cross-linking |
| title | Fabrication of alginate-based hydrogel cross-linked via horseradish peroxidase for articular cartilage engineering |
| title_full | Fabrication of alginate-based hydrogel cross-linked via horseradish peroxidase for articular cartilage engineering |
| title_fullStr | Fabrication of alginate-based hydrogel cross-linked via horseradish peroxidase for articular cartilage engineering |
| title_full_unstemmed | Fabrication of alginate-based hydrogel cross-linked via horseradish peroxidase for articular cartilage engineering |
| title_short | Fabrication of alginate-based hydrogel cross-linked via horseradish peroxidase for articular cartilage engineering |
| title_sort | fabrication of alginate based hydrogel cross linked via horseradish peroxidase for articular cartilage engineering |
| topic | Alginate Hydrogel Phenolation Enzymatic cross-linking |
| url | https://doi.org/10.1186/s13104-021-05795-2 |
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