Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings
Abstract Background Breeding rubber tree seedling with growth heterosis is vital for natural rubber production. It is the prerequisites for effectively utilizing growth heterosis to elucidate its molecular mechanisms, but the molecular mechanisms remain poorly understood in rubber tree. To elucidate...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-01-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-017-1203-3 |
| _version_ | 1819072512209715200 |
|---|---|
| author | Hong Yang Xuncheng Wang Yongxuan Wei Zhi Deng Hui Liu Jiangshu Chen Longjun Dai Zhihui Xia Guangming He Dejun Li |
| author_facet | Hong Yang Xuncheng Wang Yongxuan Wei Zhi Deng Hui Liu Jiangshu Chen Longjun Dai Zhihui Xia Guangming He Dejun Li |
| author_sort | Hong Yang |
| collection | DOAJ |
| description | Abstract Background Breeding rubber tree seedling with growth heterosis is vital for natural rubber production. It is the prerequisites for effectively utilizing growth heterosis to elucidate its molecular mechanisms, but the molecular mechanisms remain poorly understood in rubber tree. To elucidate seedling growth heterosis, we conducted comparative transcriptomic analyses between the two hybrids and their parents. Results By identifying and comparing differently expressed genes (DEGs), we found that the hybrids (BT 3410 and WC 11) show significantly differential expression profiles from their parents (PR 107 and RRIM 600). In BT 3410-parent triad, 1092 (49.95%) and 1094 (50.05%) DEGs indicated clear underdominance or overdominance, respectively. Whereas in WC 11-parent triad, most DEGs (78.2%, 721) showed low- or high-parent dominance; 160 (17.35%) exhibited expression patterns that are not statistically distinguishable from additivity, and 8 (0.87%) and 33 (3.58%) DEGs exhibited underdominance and overdominance, respectively. Furthermore, some biological processes are differentially regulated between two hybrids. Interestingly, the pathway in response to stimulus is significantly downregulated and metabolic pathways are more highly regulated in BT 3410. Conclusions Taken together, the genotypes, transcriptomes and biological pathways (especially, carbohydrate metabolism) are highly divergent between two hybrids, which may be associated with growth heterosis and weakness. Analyzing gene action models in hybrid-parent triads, we propose that overdominance may play important roles on growth heterosis, whereas dominance on hybrid weakness in rubber tree seedlings. These findings bring new insights into our understanding of growth heterosis of rubber tree seedling. |
| first_indexed | 2024-12-21T17:38:54Z |
| format | Article |
| id | doaj.art-f9595e4c595c4dba865ddfa1a61edb22 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-21T17:38:54Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-f9595e4c595c4dba865ddfa1a61edb222022-12-21T18:55:42ZengBMCBMC Plant Biology1471-22292018-01-0118111110.1186/s12870-017-1203-3Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlingsHong Yang0Xuncheng Wang1Yongxuan Wei2Zhi Deng3Hui Liu4Jiangshu Chen5Longjun Dai6Zhihui Xia7Guangming He8Dejun Li9Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural SciencesState Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agriculture Sciences and School of Life Sciences, Peking UniversityHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan UniversityKey Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural SciencesKey Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural SciencesHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan UniversityKey Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural SciencesHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan UniversityState Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agriculture Sciences and School of Life Sciences, Peking UniversityKey Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural SciencesAbstract Background Breeding rubber tree seedling with growth heterosis is vital for natural rubber production. It is the prerequisites for effectively utilizing growth heterosis to elucidate its molecular mechanisms, but the molecular mechanisms remain poorly understood in rubber tree. To elucidate seedling growth heterosis, we conducted comparative transcriptomic analyses between the two hybrids and their parents. Results By identifying and comparing differently expressed genes (DEGs), we found that the hybrids (BT 3410 and WC 11) show significantly differential expression profiles from their parents (PR 107 and RRIM 600). In BT 3410-parent triad, 1092 (49.95%) and 1094 (50.05%) DEGs indicated clear underdominance or overdominance, respectively. Whereas in WC 11-parent triad, most DEGs (78.2%, 721) showed low- or high-parent dominance; 160 (17.35%) exhibited expression patterns that are not statistically distinguishable from additivity, and 8 (0.87%) and 33 (3.58%) DEGs exhibited underdominance and overdominance, respectively. Furthermore, some biological processes are differentially regulated between two hybrids. Interestingly, the pathway in response to stimulus is significantly downregulated and metabolic pathways are more highly regulated in BT 3410. Conclusions Taken together, the genotypes, transcriptomes and biological pathways (especially, carbohydrate metabolism) are highly divergent between two hybrids, which may be associated with growth heterosis and weakness. Analyzing gene action models in hybrid-parent triads, we propose that overdominance may play important roles on growth heterosis, whereas dominance on hybrid weakness in rubber tree seedlings. These findings bring new insights into our understanding of growth heterosis of rubber tree seedling.http://link.springer.com/article/10.1186/s12870-017-1203-3Hevea brasiliensisTranscriptome analysesGrowth heterosisMolecular mechanismGene action model |
| spellingShingle | Hong Yang Xuncheng Wang Yongxuan Wei Zhi Deng Hui Liu Jiangshu Chen Longjun Dai Zhihui Xia Guangming He Dejun Li Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings BMC Plant Biology Hevea brasiliensis Transcriptome analyses Growth heterosis Molecular mechanism Gene action model |
| title | Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings |
| title_full | Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings |
| title_fullStr | Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings |
| title_full_unstemmed | Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings |
| title_short | Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings |
| title_sort | transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings |
| topic | Hevea brasiliensis Transcriptome analyses Growth heterosis Molecular mechanism Gene action model |
| url | http://link.springer.com/article/10.1186/s12870-017-1203-3 |
| work_keys_str_mv | AT hongyang transcriptomicanalysesrevealmolecularmechanismsunderlyinggrowthheterosisandweaknessofrubbertreeseedlings AT xunchengwang transcriptomicanalysesrevealmolecularmechanismsunderlyinggrowthheterosisandweaknessofrubbertreeseedlings AT yongxuanwei transcriptomicanalysesrevealmolecularmechanismsunderlyinggrowthheterosisandweaknessofrubbertreeseedlings AT zhideng transcriptomicanalysesrevealmolecularmechanismsunderlyinggrowthheterosisandweaknessofrubbertreeseedlings AT huiliu transcriptomicanalysesrevealmolecularmechanismsunderlyinggrowthheterosisandweaknessofrubbertreeseedlings AT jiangshuchen transcriptomicanalysesrevealmolecularmechanismsunderlyinggrowthheterosisandweaknessofrubbertreeseedlings AT longjundai transcriptomicanalysesrevealmolecularmechanismsunderlyinggrowthheterosisandweaknessofrubbertreeseedlings AT zhihuixia transcriptomicanalysesrevealmolecularmechanismsunderlyinggrowthheterosisandweaknessofrubbertreeseedlings AT guangminghe transcriptomicanalysesrevealmolecularmechanismsunderlyinggrowthheterosisandweaknessofrubbertreeseedlings AT dejunli transcriptomicanalysesrevealmolecularmechanismsunderlyinggrowthheterosisandweaknessofrubbertreeseedlings |