Actin Stress Fibers Response and Adaptation under Stretch
One of the many effects of soft tissues under mechanical solicitation in the cellular damage produced by highly localized strain. Here, we study the response of peripheral stress fibers (SFs) to external stretch in mammalian cells, plated onto deformable micropatterned substrates. A local fluorescen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/9/5095 |
| _version_ | 1797504458880450560 |
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| author | Roberto Bernal Milenka Van Hemelryck Basile Gurchenkov Damien Cuvelier |
| author_facet | Roberto Bernal Milenka Van Hemelryck Basile Gurchenkov Damien Cuvelier |
| author_sort | Roberto Bernal |
| collection | DOAJ |
| description | One of the many effects of soft tissues under mechanical solicitation in the cellular damage produced by highly localized strain. Here, we study the response of peripheral stress fibers (SFs) to external stretch in mammalian cells, plated onto deformable micropatterned substrates. A local fluorescence analysis reveals that an adaptation response is observed at the vicinity of the focal adhesion sites (FAs) due to its mechanosensor function. The response depends on the type of mechanical stress, from a Maxwell-type material in compression to a complex scenario in extension, where a mechanotransduction and a self-healing process takes place in order to prevent the induced severing of the SF. A model is proposed to take into account the effect of the applied stretch on the mechanics of the SF, from which relevant parameters of the healing process are obtained. In contrast, the repair of the actin bundle occurs at the weak point of the SF and depends on the amount of applied strain. As a result, the SFs display strain-softening features due to the incorporation of new actin material into the bundle. In contrast, the response under compression shows a reorganization with a constant actin material suggesting a gliding process of the SFs by the myosin II motors. |
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| format | Article |
| id | doaj.art-f959636b56d848a2a9ee51a01b8e449c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T04:04:52Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f959636b56d848a2a9ee51a01b8e449c2023-11-23T08:27:01ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-01239509510.3390/ijms23095095Actin Stress Fibers Response and Adaptation under StretchRoberto Bernal0Milenka Van Hemelryck1Basile Gurchenkov2Damien Cuvelier3Cellular Mechanics Laboratory, Physics Department, SMAT-C, Universidad de Santiago de Chile, Santiago 9170124, ChileCellular Mechanics Laboratory, Physics Department, SMAT-C, Universidad de Santiago de Chile, Santiago 9170124, ChileInstitut du Cerveau et de la Moelle Épinière, Hôpital Pitié Salpêtrière, 47 bd de l’Hôpital, 75013 Paris, FranceSorbonne Université, Faculté des Sciences et Ingénierie, UFR 926 Chemistry, 75005 Paris, FranceOne of the many effects of soft tissues under mechanical solicitation in the cellular damage produced by highly localized strain. Here, we study the response of peripheral stress fibers (SFs) to external stretch in mammalian cells, plated onto deformable micropatterned substrates. A local fluorescence analysis reveals that an adaptation response is observed at the vicinity of the focal adhesion sites (FAs) due to its mechanosensor function. The response depends on the type of mechanical stress, from a Maxwell-type material in compression to a complex scenario in extension, where a mechanotransduction and a self-healing process takes place in order to prevent the induced severing of the SF. A model is proposed to take into account the effect of the applied stretch on the mechanics of the SF, from which relevant parameters of the healing process are obtained. In contrast, the repair of the actin bundle occurs at the weak point of the SF and depends on the amount of applied strain. As a result, the SFs display strain-softening features due to the incorporation of new actin material into the bundle. In contrast, the response under compression shows a reorganization with a constant actin material suggesting a gliding process of the SFs by the myosin II motors.https://www.mdpi.com/1422-0067/23/9/5095actin stress fiberscell mechanicsSF response |
| spellingShingle | Roberto Bernal Milenka Van Hemelryck Basile Gurchenkov Damien Cuvelier Actin Stress Fibers Response and Adaptation under Stretch International Journal of Molecular Sciences actin stress fibers cell mechanics SF response |
| title | Actin Stress Fibers Response and Adaptation under Stretch |
| title_full | Actin Stress Fibers Response and Adaptation under Stretch |
| title_fullStr | Actin Stress Fibers Response and Adaptation under Stretch |
| title_full_unstemmed | Actin Stress Fibers Response and Adaptation under Stretch |
| title_short | Actin Stress Fibers Response and Adaptation under Stretch |
| title_sort | actin stress fibers response and adaptation under stretch |
| topic | actin stress fibers cell mechanics SF response |
| url | https://www.mdpi.com/1422-0067/23/9/5095 |
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