A Novel Platform for Root Protection Applies New Root-Coating Technologies to Mitigate Soil-Borne Tomato Brown Rugose Fruit Virus Disease
Tomato brown rugose fruit virus (ToBRFV) is a soil-borne virus showing a low percentage of ca. 3% soil-mediated infection when the soil contains root debris from a previous 30–50 day growth cycle of ToBRFV-infected tomato plants. We designed stringent conditions of soil-mediated ToBRFV infection by...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/3/728 |
| _version_ | 1797608661347991552 |
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| author | Eyal Klein Elisheva Smith Chen Klap Elena Bakelman Arie Ophir Aviad Sela Elena Poverenov Dmitry Rein Yachin Cohen Dan Eliahu Shai Shahal Guy Mechrez Karthik Ananth Mani Pulikanti Guruprasad Reddy Abraham J. Domb Nadav Pass Aviv Dombrovsky |
| author_facet | Eyal Klein Elisheva Smith Chen Klap Elena Bakelman Arie Ophir Aviad Sela Elena Poverenov Dmitry Rein Yachin Cohen Dan Eliahu Shai Shahal Guy Mechrez Karthik Ananth Mani Pulikanti Guruprasad Reddy Abraham J. Domb Nadav Pass Aviv Dombrovsky |
| author_sort | Eyal Klein |
| collection | DOAJ |
| description | Tomato brown rugose fruit virus (ToBRFV) is a soil-borne virus showing a low percentage of ca. 3% soil-mediated infection when the soil contains root debris from a previous 30–50 day growth cycle of ToBRFV-infected tomato plants. We designed stringent conditions of soil-mediated ToBRFV infection by increasing the length of the pre-growth cycle to 90–120 days, adding a ToBRFV inoculum as well as truncating seedling roots, which increased seedling susceptibility to ToBRFV infection. These rigorous conditions were employed to challenge the efficiency of four innovative root-coating technologies in mitigating soil-mediated ToBRFV infection while avoiding any phytotoxic effect. We tested four different formulations, which were prepared with or without the addition of various virus disinfectants. We found that under conditions of 100% soil-mediated ToBRFV infection of uncoated positive control plants, root-coating with formulations based on methylcellulose (MC), polyvinyl alcohol (PVA), silica Pickering emulsion and super-absorbent polymer (SAP) that were prepared with the disinfectant chlorinated-trisodium phosphate (Cl-TSP) showed low percentages of soil-mediated ToBRFV infection of 0%, 4.3%, 5.5% and 0%, respectively. These formulations had no adverse effect on plant growth parameters when compared to negative control plants grown under non ToBRFV inoculation conditions. |
| first_indexed | 2024-03-11T05:46:23Z |
| format | Article |
| id | doaj.art-f95d1342fc8c49078b83ab5865a5895d |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T05:46:23Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f95d1342fc8c49078b83ab5865a5895d2023-11-17T14:23:30ZengMDPI AGViruses1999-49152023-03-0115372810.3390/v15030728A Novel Platform for Root Protection Applies New Root-Coating Technologies to Mitigate Soil-Borne Tomato Brown Rugose Fruit Virus DiseaseEyal Klein0Elisheva Smith1Chen Klap2Elena Bakelman3Arie Ophir4Aviad Sela5Elena Poverenov6Dmitry Rein7Yachin Cohen8Dan Eliahu9Shai Shahal10Guy Mechrez11Karthik Ananth Mani12Pulikanti Guruprasad Reddy13Abraham J. Domb14Nadav Pass15Aviv Dombrovsky16Hishtil Nurseries, Nehalim 4995000, IsraelHishtil Nurseries, Nehalim 4995000, IsraelDepartment of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Institute, Rishon LeZion 7505101, IsraelDepartment of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Institute, Rishon LeZion 7505101, IsraelDepartment of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Institute, Rishon LeZion 7505101, IsraelThe Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 761001, IsraelAgro-Nanotechnology and Advanced Materials Center, Department of Food Science, Agricultural Research Organization, The Volcani Institute, Rishon LeZion 7505101, IsraelDepartment of Chemical Engineering, Technion—Israel Institute of Technology, Haifa 3200003, IsraelDepartment of Chemical Engineering, Technion—Israel Institute of Technology, Haifa 3200003, IsraelPolyGreen Ltd., North Industrial Zone, Nahariya 2231103, IsraelPolyGreen Ltd., North Industrial Zone, Nahariya 2231103, IsraelAgro-Nanotechnology and Advanced Materials Center, Department of Food Science, Agricultural Research Organization, The Volcani Institute, Rishon LeZion 7505101, IsraelAgro-Nanotechnology and Advanced Materials Center, Department of Food Science, Agricultural Research Organization, The Volcani Institute, Rishon LeZion 7505101, IsraelSchool of Pharmacy-Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112002, IsraelSchool of Pharmacy-Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112002, IsraelHishtil Nurseries, Nehalim 4995000, IsraelDepartment of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Institute, Rishon LeZion 7505101, IsraelTomato brown rugose fruit virus (ToBRFV) is a soil-borne virus showing a low percentage of ca. 3% soil-mediated infection when the soil contains root debris from a previous 30–50 day growth cycle of ToBRFV-infected tomato plants. We designed stringent conditions of soil-mediated ToBRFV infection by increasing the length of the pre-growth cycle to 90–120 days, adding a ToBRFV inoculum as well as truncating seedling roots, which increased seedling susceptibility to ToBRFV infection. These rigorous conditions were employed to challenge the efficiency of four innovative root-coating technologies in mitigating soil-mediated ToBRFV infection while avoiding any phytotoxic effect. We tested four different formulations, which were prepared with or without the addition of various virus disinfectants. We found that under conditions of 100% soil-mediated ToBRFV infection of uncoated positive control plants, root-coating with formulations based on methylcellulose (MC), polyvinyl alcohol (PVA), silica Pickering emulsion and super-absorbent polymer (SAP) that were prepared with the disinfectant chlorinated-trisodium phosphate (Cl-TSP) showed low percentages of soil-mediated ToBRFV infection of 0%, 4.3%, 5.5% and 0%, respectively. These formulations had no adverse effect on plant growth parameters when compared to negative control plants grown under non ToBRFV inoculation conditions.https://www.mdpi.com/1999-4915/15/3/728tobamovirussoil-mediated virus transmissionsoil disinfection |
| spellingShingle | Eyal Klein Elisheva Smith Chen Klap Elena Bakelman Arie Ophir Aviad Sela Elena Poverenov Dmitry Rein Yachin Cohen Dan Eliahu Shai Shahal Guy Mechrez Karthik Ananth Mani Pulikanti Guruprasad Reddy Abraham J. Domb Nadav Pass Aviv Dombrovsky A Novel Platform for Root Protection Applies New Root-Coating Technologies to Mitigate Soil-Borne Tomato Brown Rugose Fruit Virus Disease Viruses tobamovirus soil-mediated virus transmission soil disinfection |
| title | A Novel Platform for Root Protection Applies New Root-Coating Technologies to Mitigate Soil-Borne Tomato Brown Rugose Fruit Virus Disease |
| title_full | A Novel Platform for Root Protection Applies New Root-Coating Technologies to Mitigate Soil-Borne Tomato Brown Rugose Fruit Virus Disease |
| title_fullStr | A Novel Platform for Root Protection Applies New Root-Coating Technologies to Mitigate Soil-Borne Tomato Brown Rugose Fruit Virus Disease |
| title_full_unstemmed | A Novel Platform for Root Protection Applies New Root-Coating Technologies to Mitigate Soil-Borne Tomato Brown Rugose Fruit Virus Disease |
| title_short | A Novel Platform for Root Protection Applies New Root-Coating Technologies to Mitigate Soil-Borne Tomato Brown Rugose Fruit Virus Disease |
| title_sort | novel platform for root protection applies new root coating technologies to mitigate soil borne tomato brown rugose fruit virus disease |
| topic | tobamovirus soil-mediated virus transmission soil disinfection |
| url | https://www.mdpi.com/1999-4915/15/3/728 |
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