Expression of hyaluronan synthase 3 in deformed human temporomandibular joint discs: in vivo and in vitro studies
The present study aimed at investigating the expression of a hyaluronan synthase (HAS) 3 in tissue samples of deformed human temporomandibular joint (TMJ) discs and cells obtained from the discs. Fifteen adult human TMJ discs (twelve diseased discs and three normal discs) were used in this study. Th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2010-12-01
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| Series: | European Journal of Histochemistry |
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| Online Access: | http://www.ejh.it/index.php/ejh/article/view/1781 |
| _version_ | 1818055336134705152 |
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| author | T. Matsumoto M. Inayama I. Tojyo N. Kiga S. Fujita |
| author_facet | T. Matsumoto M. Inayama I. Tojyo N. Kiga S. Fujita |
| author_sort | T. Matsumoto |
| collection | DOAJ |
| description | The present study aimed at investigating the expression of a hyaluronan synthase (HAS) 3 in tissue samples of deformed human temporomandibular joint (TMJ) discs and cells obtained from the discs. Fifteen adult human TMJ discs (twelve diseased discs and three normal discs) were used in this study. The twelve diseased discs were obtained from twelve patients with internal derangement (ID) of TMJ. These patients all had anteriorly displaced discs and deformed discs. The tissues were immunohistochemically stained using HAS3 antibodies. In addition, the subcultured TMJ disc cells under both normal and hypoxic conditions (O2: 2%) were incubated for 3, 6, 12, and 24 h after addition of interleukin-1ß (IL-1ß) (1ng/mL). Subsequently, the expression of HAS3 was examined using real-time reverse transcription-polymerase chain reaction (RT-PCR). The control group showed from negative to weak positive reactions for HAS3 on immunohistochemical staining. The discs extracted from twelve cases with ID presented from moderate to strong positive reactions for HAS3. The quantity of HAS3 mRNA was compared with a control group, and showed a 204-fold increase at 3 h, a 26-fold increase at 6 h, a 2.5-fold increase at 12 h and a 32-fold increase at 24 h under hypoxia with the addition of IL-1ß. The expression of HAS3 mRNA was significantly enhanced at 3 h and 24 h. The results obtained suggest that HAS3 is related to the pathological changes of human TMJ discs affected by ID. |
| first_indexed | 2024-12-10T12:11:19Z |
| format | Article |
| id | doaj.art-f9633e507519491e8b34b28f6f5550a9 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-10T12:11:19Z |
| publishDate | 2010-12-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-f9633e507519491e8b34b28f6f5550a92022-12-22T01:49:22ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062010-12-01544e50e5010.4081/ejh.2010.e501171Expression of hyaluronan synthase 3 in deformed human temporomandibular joint discs: in vivo and in vitro studiesT. MatsumotoM. InayamaI. TojyoN. KigaS. FujitaThe present study aimed at investigating the expression of a hyaluronan synthase (HAS) 3 in tissue samples of deformed human temporomandibular joint (TMJ) discs and cells obtained from the discs. Fifteen adult human TMJ discs (twelve diseased discs and three normal discs) were used in this study. The twelve diseased discs were obtained from twelve patients with internal derangement (ID) of TMJ. These patients all had anteriorly displaced discs and deformed discs. The tissues were immunohistochemically stained using HAS3 antibodies. In addition, the subcultured TMJ disc cells under both normal and hypoxic conditions (O2: 2%) were incubated for 3, 6, 12, and 24 h after addition of interleukin-1ß (IL-1ß) (1ng/mL). Subsequently, the expression of HAS3 was examined using real-time reverse transcription-polymerase chain reaction (RT-PCR). The control group showed from negative to weak positive reactions for HAS3 on immunohistochemical staining. The discs extracted from twelve cases with ID presented from moderate to strong positive reactions for HAS3. The quantity of HAS3 mRNA was compared with a control group, and showed a 204-fold increase at 3 h, a 26-fold increase at 6 h, a 2.5-fold increase at 12 h and a 32-fold increase at 24 h under hypoxia with the addition of IL-1ß. The expression of HAS3 mRNA was significantly enhanced at 3 h and 24 h. The results obtained suggest that HAS3 is related to the pathological changes of human TMJ discs affected by ID.http://www.ejh.it/index.php/ejh/article/view/1781Temporomandibular joint discInternal derangementHyaluronan synthase 3 (HAS3)HumanHypoxia |
| spellingShingle | T. Matsumoto M. Inayama I. Tojyo N. Kiga S. Fujita Expression of hyaluronan synthase 3 in deformed human temporomandibular joint discs: in vivo and in vitro studies European Journal of Histochemistry Temporomandibular joint disc Internal derangement Hyaluronan synthase 3 (HAS3) Human Hypoxia |
| title | Expression of hyaluronan synthase 3 in deformed human temporomandibular joint discs: in vivo and in vitro studies |
| title_full | Expression of hyaluronan synthase 3 in deformed human temporomandibular joint discs: in vivo and in vitro studies |
| title_fullStr | Expression of hyaluronan synthase 3 in deformed human temporomandibular joint discs: in vivo and in vitro studies |
| title_full_unstemmed | Expression of hyaluronan synthase 3 in deformed human temporomandibular joint discs: in vivo and in vitro studies |
| title_short | Expression of hyaluronan synthase 3 in deformed human temporomandibular joint discs: in vivo and in vitro studies |
| title_sort | expression of hyaluronan synthase 3 in deformed human temporomandibular joint discs in vivo and in vitro studies |
| topic | Temporomandibular joint disc Internal derangement Hyaluronan synthase 3 (HAS3) Human Hypoxia |
| url | http://www.ejh.it/index.php/ejh/article/view/1781 |
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