A comparison of machine learning methods for quantifying self-grooming behavior in mice
BackgroundAs machine learning technology continues to advance and the need for standardized behavioral quantification grows, commercial and open-source automated behavioral analysis tools are gaining prominence in behavioral neuroscience. We present a comparative analysis of three behavioral analysi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnbeh.2024.1340357/full |
| _version_ | 1797341251551363072 |
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| author | Kassi Correia Raegan Walker Christopher Pittenger Christopher Fields |
| author_facet | Kassi Correia Raegan Walker Christopher Pittenger Christopher Fields |
| author_sort | Kassi Correia |
| collection | DOAJ |
| description | BackgroundAs machine learning technology continues to advance and the need for standardized behavioral quantification grows, commercial and open-source automated behavioral analysis tools are gaining prominence in behavioral neuroscience. We present a comparative analysis of three behavioral analysis pipelines—DeepLabCut (DLC) and Simple Behavioral Analysis (SimBA), HomeCageScan (HCS), and manual scoring—in measuring repetitive self-grooming among mice.MethodsGrooming behavior of mice was recorded at baseline and after water spray or restraint treatments. Videos were processed and analyzed in parallel using 3 methods (DLC/SimBA, HCS, and manual scoring), quantifying both total number of grooming bouts and total grooming duration.ResultsBoth treatment conditions (water spray and restraint) resulted in significant elevation in both total grooming duration and number of grooming bouts. HCS measures of grooming duration were significantly elevated relative to those derived from manual scoring: specifically, HCS tended to overestimate duration at low levels of grooming. DLC/SimBA duration measurements were not significantly different than those derived from manual scoring. However, both SimBA and HCS measures of the number of grooming bouts were significantly different than those derived from manual scoring; the magnitude and direction of the difference depended on treatment condition.ConclusionDLC/SimBA provides a high-throughput pipeline for quantifying grooming duration that correlates well with manual scoring. However, grooming bout data derived from both DLC/SimBA and HCS did not reliably estimate measures obtained via manual scoring. |
| first_indexed | 2024-03-08T10:15:13Z |
| format | Article |
| id | doaj.art-f9635595572e46939d95f7e6be675f76 |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-03-08T10:15:13Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-f9635595572e46939d95f7e6be675f762024-01-29T04:22:29ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532024-01-011810.3389/fnbeh.2024.13403571340357A comparison of machine learning methods for quantifying self-grooming behavior in miceKassi Correia0Raegan Walker1Christopher Pittenger2Christopher Fields3Department of Psychiatry, Yale School of Medicine, Yale University, New Haven, CT, United StatesDepartment of Psychiatry, Yale School of Medicine, Yale University, New Haven, CT, United StatesDepartment of Psychiatry, Yale School of Medicine, New Haven, CT, United StatesDepartment of Psychiatry, Yale School of Medicine, New Haven, CT, United StatesBackgroundAs machine learning technology continues to advance and the need for standardized behavioral quantification grows, commercial and open-source automated behavioral analysis tools are gaining prominence in behavioral neuroscience. We present a comparative analysis of three behavioral analysis pipelines—DeepLabCut (DLC) and Simple Behavioral Analysis (SimBA), HomeCageScan (HCS), and manual scoring—in measuring repetitive self-grooming among mice.MethodsGrooming behavior of mice was recorded at baseline and after water spray or restraint treatments. Videos were processed and analyzed in parallel using 3 methods (DLC/SimBA, HCS, and manual scoring), quantifying both total number of grooming bouts and total grooming duration.ResultsBoth treatment conditions (water spray and restraint) resulted in significant elevation in both total grooming duration and number of grooming bouts. HCS measures of grooming duration were significantly elevated relative to those derived from manual scoring: specifically, HCS tended to overestimate duration at low levels of grooming. DLC/SimBA duration measurements were not significantly different than those derived from manual scoring. However, both SimBA and HCS measures of the number of grooming bouts were significantly different than those derived from manual scoring; the magnitude and direction of the difference depended on treatment condition.ConclusionDLC/SimBA provides a high-throughput pipeline for quantifying grooming duration that correlates well with manual scoring. However, grooming bout data derived from both DLC/SimBA and HCS did not reliably estimate measures obtained via manual scoring.https://www.frontiersin.org/articles/10.3389/fnbeh.2024.1340357/fullbehaviorHomeCageScanARBmachine learninggrooming |
| spellingShingle | Kassi Correia Raegan Walker Christopher Pittenger Christopher Fields A comparison of machine learning methods for quantifying self-grooming behavior in mice Frontiers in Behavioral Neuroscience behavior HomeCageScan ARB machine learning grooming |
| title | A comparison of machine learning methods for quantifying self-grooming behavior in mice |
| title_full | A comparison of machine learning methods for quantifying self-grooming behavior in mice |
| title_fullStr | A comparison of machine learning methods for quantifying self-grooming behavior in mice |
| title_full_unstemmed | A comparison of machine learning methods for quantifying self-grooming behavior in mice |
| title_short | A comparison of machine learning methods for quantifying self-grooming behavior in mice |
| title_sort | comparison of machine learning methods for quantifying self grooming behavior in mice |
| topic | behavior HomeCageScan ARB machine learning grooming |
| url | https://www.frontiersin.org/articles/10.3389/fnbeh.2024.1340357/full |
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