Population Genetic Structure of <i>Anisakis simplex</i> Infecting the European Hake from North East Atlantic Fishing Grounds
The European hake, one of the most commercially valuable species in ICES fishing areas, is considered an important neglected source of zoonotic risk by nematode parasites belonging to the genus <i>Anisakis</i>. <i>Merluccius merluccius</i> is, by far, the most important host...
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MDPI AG
2023-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/2/197 |
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| author | Andrea Ramilo Helena Rodríguez Santiago Pascual Ángel F. González Elvira Abollo |
| author_facet | Andrea Ramilo Helena Rodríguez Santiago Pascual Ángel F. González Elvira Abollo |
| author_sort | Andrea Ramilo |
| collection | DOAJ |
| description | The European hake, one of the most commercially valuable species in ICES fishing areas, is considered an important neglected source of zoonotic risk by nematode parasites belonging to the genus <i>Anisakis</i>. <i>Merluccius merluccius</i> is, by far, the most important host of <i>Anisakis</i> spp. at the European fishing grounds, in terms of demographic infection values, and carries the highest parasite burden. These high parasite population densities within an individual fish host offer a chance to explore new sources of variations for the genetic structure of <i>Anisakis</i> spp. populations. A total of 873 <i>Anisakis</i> spp. third-stage larvae, originally sampled from viscera and muscular sections of hake collected at ten fishing grounds, were primarily identified using ITS rDNA region as molecular marker. After that, we used mtDNA <i>cox2</i> gene to reveal the high haplotype diversity and the lack of genetic structure for <i>A. simplex</i>. Dominant haplotypes were shared among the different fishing areas and fish sections analyzed. Results indicate a clear connection of <i>A. simplex</i> from European hake along the Northern North Sea to the Portuguese coast, constituting a single genetic population but revealing a certain level of genetic sub-structuring on the Northwest coast of Scotland. This study also provides useful information to advance the understanding of parasite speciation to different fish host tissues or microenvironments. |
| first_indexed | 2024-03-09T13:49:38Z |
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| id | doaj.art-f964f6cc161e47429a97d43d36b9cb3b |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T13:49:38Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-f964f6cc161e47429a97d43d36b9cb3b2023-11-30T20:52:34ZengMDPI AGAnimals2076-26152023-01-0113219710.3390/ani13020197Population Genetic Structure of <i>Anisakis simplex</i> Infecting the European Hake from North East Atlantic Fishing GroundsAndrea Ramilo0Helena Rodríguez1Santiago Pascual2Ángel F. González3Elvira Abollo4Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, IIM-CSIC, 36208 Vigo, Pontevedra, SpainInstituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, IIM-CSIC, 36208 Vigo, Pontevedra, SpainInstituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, IIM-CSIC, 36208 Vigo, Pontevedra, SpainInstituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, IIM-CSIC, 36208 Vigo, Pontevedra, SpainInstituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, IIM-CSIC, 36208 Vigo, Pontevedra, SpainThe European hake, one of the most commercially valuable species in ICES fishing areas, is considered an important neglected source of zoonotic risk by nematode parasites belonging to the genus <i>Anisakis</i>. <i>Merluccius merluccius</i> is, by far, the most important host of <i>Anisakis</i> spp. at the European fishing grounds, in terms of demographic infection values, and carries the highest parasite burden. These high parasite population densities within an individual fish host offer a chance to explore new sources of variations for the genetic structure of <i>Anisakis</i> spp. populations. A total of 873 <i>Anisakis</i> spp. third-stage larvae, originally sampled from viscera and muscular sections of hake collected at ten fishing grounds, were primarily identified using ITS rDNA region as molecular marker. After that, we used mtDNA <i>cox2</i> gene to reveal the high haplotype diversity and the lack of genetic structure for <i>A. simplex</i>. Dominant haplotypes were shared among the different fishing areas and fish sections analyzed. Results indicate a clear connection of <i>A. simplex</i> from European hake along the Northern North Sea to the Portuguese coast, constituting a single genetic population but revealing a certain level of genetic sub-structuring on the Northwest coast of Scotland. This study also provides useful information to advance the understanding of parasite speciation to different fish host tissues or microenvironments.https://www.mdpi.com/2076-2615/13/2/197<i>Anisakis simplex</i><i>Anisakis pegreffii</i>hybrid genotypeEuropean hakegenetic structure |
| spellingShingle | Andrea Ramilo Helena Rodríguez Santiago Pascual Ángel F. González Elvira Abollo Population Genetic Structure of <i>Anisakis simplex</i> Infecting the European Hake from North East Atlantic Fishing Grounds Animals <i>Anisakis simplex</i> <i>Anisakis pegreffii</i> hybrid genotype European hake genetic structure |
| title | Population Genetic Structure of <i>Anisakis simplex</i> Infecting the European Hake from North East Atlantic Fishing Grounds |
| title_full | Population Genetic Structure of <i>Anisakis simplex</i> Infecting the European Hake from North East Atlantic Fishing Grounds |
| title_fullStr | Population Genetic Structure of <i>Anisakis simplex</i> Infecting the European Hake from North East Atlantic Fishing Grounds |
| title_full_unstemmed | Population Genetic Structure of <i>Anisakis simplex</i> Infecting the European Hake from North East Atlantic Fishing Grounds |
| title_short | Population Genetic Structure of <i>Anisakis simplex</i> Infecting the European Hake from North East Atlantic Fishing Grounds |
| title_sort | population genetic structure of i anisakis simplex i infecting the european hake from north east atlantic fishing grounds |
| topic | <i>Anisakis simplex</i> <i>Anisakis pegreffii</i> hybrid genotype European hake genetic structure |
| url | https://www.mdpi.com/2076-2615/13/2/197 |
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