Cytotoxicity assessment of Bio-C Repair Íon+: A new calcium silicate-based cement
Background. Direct pulp capping is a method designed to preserve the exposed dental pulp. Due to good biological, physical, and mechanical properties, new versions of calcium silicate-based materials have been developed as pulp capping materials. The present study aimed to evaluate the cytotoxic eff...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2021-08-01
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| Series: | Journal of Dental Research, Dental Clinics, Dental Prospects |
| Subjects: | |
| Online Access: | https://joddd.tbzmed.ac.ir/PDF/joddd-15-152.pdf |
| _version_ | 1818582537963831296 |
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| author | Celso Afonso Klein-Junior Roberto Zimmer Tãnyre Dobler Vanessa Oliveira Daniel Rodrigo Marinowic Ahmet Özkömür Eduardo Galia Reston |
| author_facet | Celso Afonso Klein-Junior Roberto Zimmer Tãnyre Dobler Vanessa Oliveira Daniel Rodrigo Marinowic Ahmet Özkömür Eduardo Galia Reston |
| author_sort | Celso Afonso Klein-Junior |
| collection | DOAJ |
| description | Background. Direct pulp capping is a method designed to preserve the exposed dental pulp. Due to good biological, physical, and mechanical properties, new versions of calcium silicate-based materials have been developed as pulp capping materials. The present study aimed to evaluate the cytotoxic effects of four calcium silicate-based pulp capping materials, of which the Bio-C Repair Íon+ is still in an experimental phase. Methods. Biodentine, MTA Repair HP, Bio-C Repair, and Bio-C Repair Íon+ cements were dispensed in a metallic matrix to produce 125-mm3 specimens, which were immersed in Dulbecco’s Modified Eagle Medium (DMEM) to obtain extracts. NIH 3T3 cells were cultured and exposed to the extracts for 24 hours and seven days. Cell viability was assessed by the methyl tetrazolium test (MTT). The mean values for the experimental and control groups (without treatment) were compared by analysis of variance (ANOVA) and post hoc Tukey tests, considering a significance level of 5%. Results. All the tested materials demonstrated a reduction in cell viability (P<0.05). According to ISO 10993-5: 2009 (E), Bio-C Repair Íon+ exhibited mild and moderate cytotoxicity in the 24- hour and 7-day analyses, respectively. Bio-C Repair and Biodentine showed mild cytotoxicity, and MTA Repair HP exhibited moderate cytotoxicity at both intervals. Conclusion. The highest cell viability was demonstrated by Biodentine, MTA, and Repair HP, in descending order. Bio-C Repair and Bio-C Repair Íon+ showed moderate cytotoxicity, similar to MTA Repair HP in the 7-day analysis. |
| first_indexed | 2024-12-16T07:50:58Z |
| format | Article |
| id | doaj.art-f9656d5e4ae9439288719f260922acaa |
| institution | Directory Open Access Journal |
| issn | 2008-210X 2008-2118 |
| language | English |
| last_indexed | 2024-12-16T07:50:58Z |
| publishDate | 2021-08-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Journal of Dental Research, Dental Clinics, Dental Prospects |
| spelling | doaj.art-f9656d5e4ae9439288719f260922acaa2022-12-21T22:38:53ZengTabriz University of Medical SciencesJournal of Dental Research, Dental Clinics, Dental Prospects2008-210X2008-21182021-08-0115315215610.34172/joddd.2021.026joddd-29737Cytotoxicity assessment of Bio-C Repair Íon+: A new calcium silicate-based cementCelso Afonso Klein-Junior0Roberto Zimmer1Tãnyre Dobler2Vanessa Oliveira3Daniel Rodrigo Marinowic4Ahmet Özkömür5Eduardo Galia Reston6Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil, Canoas, RS, BrazilDepartment of Operative Dentistry, School of Dentistry, Lutheran University of Brazil, Canoas, RS, BrazilDepartment of Operative Dentistry, School of Dentistry, Lutheran University of Brazil, Cachoeira do Sul, RS, BrazilDepartment of Operative Dentistry, School of Dentistry, Lutheran University of Brazil, Cachoeira do Sul, RS, BrazilBrain Institute, Neuroscience laboratory, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, BrazilDepartment of Operative Dentistry, School of Dentistry, Lutheran University of Brazil, Canoas, RS, BrazilDepartment of Operative Dentistry, School of Dentistry, Lutheran University of Brazil, Canoas, RS, BrazilBackground. Direct pulp capping is a method designed to preserve the exposed dental pulp. Due to good biological, physical, and mechanical properties, new versions of calcium silicate-based materials have been developed as pulp capping materials. The present study aimed to evaluate the cytotoxic effects of four calcium silicate-based pulp capping materials, of which the Bio-C Repair Íon+ is still in an experimental phase. Methods. Biodentine, MTA Repair HP, Bio-C Repair, and Bio-C Repair Íon+ cements were dispensed in a metallic matrix to produce 125-mm3 specimens, which were immersed in Dulbecco’s Modified Eagle Medium (DMEM) to obtain extracts. NIH 3T3 cells were cultured and exposed to the extracts for 24 hours and seven days. Cell viability was assessed by the methyl tetrazolium test (MTT). The mean values for the experimental and control groups (without treatment) were compared by analysis of variance (ANOVA) and post hoc Tukey tests, considering a significance level of 5%. Results. All the tested materials demonstrated a reduction in cell viability (P<0.05). According to ISO 10993-5: 2009 (E), Bio-C Repair Íon+ exhibited mild and moderate cytotoxicity in the 24- hour and 7-day analyses, respectively. Bio-C Repair and Biodentine showed mild cytotoxicity, and MTA Repair HP exhibited moderate cytotoxicity at both intervals. Conclusion. The highest cell viability was demonstrated by Biodentine, MTA, and Repair HP, in descending order. Bio-C Repair and Bio-C Repair Íon+ showed moderate cytotoxicity, similar to MTA Repair HP in the 7-day analysis.https://joddd.tbzmed.ac.ir/PDF/joddd-15-152.pdfcalcium silicatecell survivalfibroblastsmineral trioxide aggregate |
| spellingShingle | Celso Afonso Klein-Junior Roberto Zimmer Tãnyre Dobler Vanessa Oliveira Daniel Rodrigo Marinowic Ahmet Özkömür Eduardo Galia Reston Cytotoxicity assessment of Bio-C Repair Íon+: A new calcium silicate-based cement Journal of Dental Research, Dental Clinics, Dental Prospects calcium silicate cell survival fibroblasts mineral trioxide aggregate |
| title | Cytotoxicity assessment of Bio-C Repair Íon+: A new calcium silicate-based cement |
| title_full | Cytotoxicity assessment of Bio-C Repair Íon+: A new calcium silicate-based cement |
| title_fullStr | Cytotoxicity assessment of Bio-C Repair Íon+: A new calcium silicate-based cement |
| title_full_unstemmed | Cytotoxicity assessment of Bio-C Repair Íon+: A new calcium silicate-based cement |
| title_short | Cytotoxicity assessment of Bio-C Repair Íon+: A new calcium silicate-based cement |
| title_sort | cytotoxicity assessment of bio c repair ion a new calcium silicate based cement |
| topic | calcium silicate cell survival fibroblasts mineral trioxide aggregate |
| url | https://joddd.tbzmed.ac.ir/PDF/joddd-15-152.pdf |
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