Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2
Abstract Squamous cell carcinoma of the external auditory canal (EACSCC) is an extraordinarily rare and aggressive malignant disease. Establishment of EACSCC cell line with robust molecular characteristics is essential for the basic and translational research of EACSCC. Here, we show the newly estab...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-46926-y |
| _version_ | 1827764471345446912 |
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| author | Noritaka Komune Kuniaki Sato Mayumi Ono Akira Imaizumi Shogo Masuda Shinsaku Itoyama Tomomi Manako Ryosuke Kuga Takahiro Hongo Ryunosuke Kogo Hideya Onishi Takashi Nakagawa |
| author_facet | Noritaka Komune Kuniaki Sato Mayumi Ono Akira Imaizumi Shogo Masuda Shinsaku Itoyama Tomomi Manako Ryosuke Kuga Takahiro Hongo Ryunosuke Kogo Hideya Onishi Takashi Nakagawa |
| author_sort | Noritaka Komune |
| collection | DOAJ |
| description | Abstract Squamous cell carcinoma of the external auditory canal (EACSCC) is an extraordinarily rare and aggressive malignant disease. Establishment of EACSCC cell line with robust molecular characteristics is essential for the basic and translational research of EACSCC. Here, we show the newly established EACSCC cell line SCEACono2, derived from a patient with well-to-moderately differentiated EACSCC. We analyzed histologic and genetic features of SCEACono2 hiring multiple experiments, including next-generation sequencing (NGS). Immunocytochemical staining of SCEACono2 showed positivity of p53 and SCC1/2. Furthermore, SCEACono2 exhibited a unique characteristic that cytokeratin, vimentin as well as cancer stem cell markers (CD44, CD133, ALP and Oct3/4) were positive. SCEACono2 had an ability to form tumors at the temporal lesion xenograft nude mice model. NGS revealed that SCEACono2 harbored the somatic mutations of TP53 (p.G245S) and NOTCH1 (p.A465T). RNA-seq and downstream bioinformatics analysis revealed significant enrichment of genes involved in inflammation and cell adhesion in SCEACono2 compared to SCC-9 and HSC-4. STR profiling indicated no evidence of cross-contamination. In conclusion, SCEACono2 could serves as a promising and robust research resource of EACSCC in vitro and in vivo. |
| first_indexed | 2024-03-11T11:04:54Z |
| format | Article |
| id | doaj.art-f96588624d934622a39d349174d71508 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-11T11:04:54Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f96588624d934622a39d349174d715082023-11-12T12:16:51ZengNature PortfolioScientific Reports2045-23222023-11-0113111210.1038/s41598-023-46926-yBiological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2Noritaka Komune0Kuniaki Sato1Mayumi Ono2Akira Imaizumi3Shogo Masuda4Shinsaku Itoyama5Tomomi Manako6Ryosuke Kuga7Takahiro Hongo8Ryunosuke Kogo9Hideya Onishi10Takashi Nakagawa11Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu UniversityDepartment of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu UniversityDepartment of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu UniversityDepartment of Cancer Therapy and Research, Graduate School of Medical Sciences, Kyushu UniversityDepartment of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu UniversityDepartment of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu UniversityDepartment of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu UniversityDepartment of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu UniversityDepartment of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu UniversityDepartment of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu UniversityDepartment of Cancer Therapy and Research, Graduate School of Medical Sciences, Kyushu UniversityDepartment of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu UniversityAbstract Squamous cell carcinoma of the external auditory canal (EACSCC) is an extraordinarily rare and aggressive malignant disease. Establishment of EACSCC cell line with robust molecular characteristics is essential for the basic and translational research of EACSCC. Here, we show the newly established EACSCC cell line SCEACono2, derived from a patient with well-to-moderately differentiated EACSCC. We analyzed histologic and genetic features of SCEACono2 hiring multiple experiments, including next-generation sequencing (NGS). Immunocytochemical staining of SCEACono2 showed positivity of p53 and SCC1/2. Furthermore, SCEACono2 exhibited a unique characteristic that cytokeratin, vimentin as well as cancer stem cell markers (CD44, CD133, ALP and Oct3/4) were positive. SCEACono2 had an ability to form tumors at the temporal lesion xenograft nude mice model. NGS revealed that SCEACono2 harbored the somatic mutations of TP53 (p.G245S) and NOTCH1 (p.A465T). RNA-seq and downstream bioinformatics analysis revealed significant enrichment of genes involved in inflammation and cell adhesion in SCEACono2 compared to SCC-9 and HSC-4. STR profiling indicated no evidence of cross-contamination. In conclusion, SCEACono2 could serves as a promising and robust research resource of EACSCC in vitro and in vivo.https://doi.org/10.1038/s41598-023-46926-y |
| spellingShingle | Noritaka Komune Kuniaki Sato Mayumi Ono Akira Imaizumi Shogo Masuda Shinsaku Itoyama Tomomi Manako Ryosuke Kuga Takahiro Hongo Ryunosuke Kogo Hideya Onishi Takashi Nakagawa Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2 Scientific Reports |
| title | Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2 |
| title_full | Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2 |
| title_fullStr | Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2 |
| title_full_unstemmed | Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2 |
| title_short | Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2 |
| title_sort | biological and genetic characterization of a newly established human external auditory canal carcinoma cell line sceacono2 |
| url | https://doi.org/10.1038/s41598-023-46926-y |
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