Protein Chips for Detection of Salmonella spp. from Enrichment Culture
Food pathogens are the cause of foodborne epidemics, therefore there is a need to detect the pathogens in food productions rapidly. A pre-enrichment culture followed by selective agar plating are standard detection methods. Molecular methods such as qPCR have provided a first rapid protocol for dete...
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| Format: | Article |
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MDPI AG
2016-04-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/16/4/574 |
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| author | Palmiro Poltronieri Fabio Cimaglia Enrico De Lorenzis Maurizio Chiesa Valeria Mezzolla Ida Barbara Reca |
| author_facet | Palmiro Poltronieri Fabio Cimaglia Enrico De Lorenzis Maurizio Chiesa Valeria Mezzolla Ida Barbara Reca |
| author_sort | Palmiro Poltronieri |
| collection | DOAJ |
| description | Food pathogens are the cause of foodborne epidemics, therefore there is a need to detect the pathogens in food productions rapidly. A pre-enrichment culture followed by selective agar plating are standard detection methods. Molecular methods such as qPCR have provided a first rapid protocol for detection of pathogens within 24 h of enrichment culture. Biosensors also may provide a rapid tool to individuate a source of Salmonella contamination at early times of pre-enrichment culture. Forty mL of Salmonella spp. enrichment culture were processed by immunoseparation using the Pathatrix, as in AFNOR validated qPCR protocols. The Salmonella biosensor combined with immunoseparation showed a limit of detection of 100 bacteria/40 mL, with a 400 fold increase to previous results. qPCR analysis requires processing of bead-bound bacteria with lysis buffer and DNA clean up, with a limit of detection of 2 cfu/50 μL. Finally, a protein chip was developed and tested in screening and identification of 5 common pathogen species, Salmonella spp., E. coli, S. aureus, Campylobacter spp. and Listeria spp. The protein chip, with high specificity in species identification, is proposed to be integrated into a Lab-on-Chip system, for rapid and reproducible screening of Salmonella spp. and other pathogen species contaminating food productions. |
| first_indexed | 2024-04-11T22:09:26Z |
| format | Article |
| id | doaj.art-f968070226d94359bbd4ccb50dbf7273 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T22:09:26Z |
| publishDate | 2016-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-f968070226d94359bbd4ccb50dbf72732022-12-22T04:00:36ZengMDPI AGSensors1424-82202016-04-0116457410.3390/s16040574s16040574Protein Chips for Detection of Salmonella spp. from Enrichment CulturePalmiro Poltronieri0Fabio Cimaglia1Enrico De Lorenzis2Maurizio Chiesa3Valeria Mezzolla4Ida Barbara Reca5CNR-ISPA, Institute of Sciences of Food Productions, via Monteroni km 7, 73100 Lecce, ItalyBiotecgen, c/o Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyBiotecgen, c/o Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyBiotecgen, c/o Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyCNR-ISPA, Institute of Sciences of Food Productions, via Monteroni km 7, 73100 Lecce, ItalyCNR-ISPA, Institute of Sciences of Food Productions, via Monteroni km 7, 73100 Lecce, ItalyFood pathogens are the cause of foodborne epidemics, therefore there is a need to detect the pathogens in food productions rapidly. A pre-enrichment culture followed by selective agar plating are standard detection methods. Molecular methods such as qPCR have provided a first rapid protocol for detection of pathogens within 24 h of enrichment culture. Biosensors also may provide a rapid tool to individuate a source of Salmonella contamination at early times of pre-enrichment culture. Forty mL of Salmonella spp. enrichment culture were processed by immunoseparation using the Pathatrix, as in AFNOR validated qPCR protocols. The Salmonella biosensor combined with immunoseparation showed a limit of detection of 100 bacteria/40 mL, with a 400 fold increase to previous results. qPCR analysis requires processing of bead-bound bacteria with lysis buffer and DNA clean up, with a limit of detection of 2 cfu/50 μL. Finally, a protein chip was developed and tested in screening and identification of 5 common pathogen species, Salmonella spp., E. coli, S. aureus, Campylobacter spp. and Listeria spp. The protein chip, with high specificity in species identification, is proposed to be integrated into a Lab-on-Chip system, for rapid and reproducible screening of Salmonella spp. and other pathogen species contaminating food productions.http://www.mdpi.com/1424-8220/16/4/574antibodybiosensorsdetectionfood pathogensidentificationlabellingSalmonella spp. |
| spellingShingle | Palmiro Poltronieri Fabio Cimaglia Enrico De Lorenzis Maurizio Chiesa Valeria Mezzolla Ida Barbara Reca Protein Chips for Detection of Salmonella spp. from Enrichment Culture Sensors antibody biosensors detection food pathogens identification labelling Salmonella spp. |
| title | Protein Chips for Detection of Salmonella spp. from Enrichment Culture |
| title_full | Protein Chips for Detection of Salmonella spp. from Enrichment Culture |
| title_fullStr | Protein Chips for Detection of Salmonella spp. from Enrichment Culture |
| title_full_unstemmed | Protein Chips for Detection of Salmonella spp. from Enrichment Culture |
| title_short | Protein Chips for Detection of Salmonella spp. from Enrichment Culture |
| title_sort | protein chips for detection of salmonella spp from enrichment culture |
| topic | antibody biosensors detection food pathogens identification labelling Salmonella spp. |
| url | http://www.mdpi.com/1424-8220/16/4/574 |
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