Nickel Increases Chromosomal Abnormalities by Interfering With the Initiation of DNA Repair Pathways
Background: Nickel is a carcinogenic, heavy metal released through industrial activities and via natural resources. It is able to cause DNA damages by reducing the efficiency of DNA repair mechanisms. However, the exact time point at which it is able to interfere with these mechanisms is not yet cle...
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| Format: | Article |
| Language: | English |
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Arak University of Medical Sciences
2022-10-01
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| Series: | Iranian Journal of Toxicology |
| Subjects: | |
| Online Access: | http://ijt.arakmu.ac.ir/article-1-1107-en.html |
| _version_ | 1828127815032111104 |
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| author | Mahsa Ghorbani Farhang Haddad Khadijeh Shahrokhabadi |
| author_facet | Mahsa Ghorbani Farhang Haddad Khadijeh Shahrokhabadi |
| author_sort | Mahsa Ghorbani |
| collection | DOAJ |
| description | Background: Nickel is a carcinogenic, heavy metal released through industrial activities and via natural resources. It is able to cause DNA damages by reducing the efficiency of DNA repair mechanisms. However, the exact time point at which it is able to interfere with these mechanisms is not yet clearly understood.
Methods: To find the most nickel-vulnerable time of repair mechanisms, human dermal fibroblasts (HDF) were treated with three doses of nickel before and after X-irradiation. The induced frequency of chromosomal abnormality was studied using micronucleus assay in binucleated cells. The cytotoxicity of different treatments was established using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
Results: The results revealed that nickel treatment had a synergistic effect on inducing Micronucleus frequency only when cells were treated 2 hours before X-irradiation. The X-ray treatment of the cells with 5 and 10 mM nickel had a cytotoxic effect mainly when given 6 hours after the irradiation.
Conclusion: The results suggest that nickel can interfere with human DNA repair mechanisms only at the start of the process, while having no significant effect on the human DNA repair mechanisms when activated. |
| first_indexed | 2024-04-11T15:55:14Z |
| format | Article |
| id | doaj.art-f96adfbcd89547a195ab750d8cf2cc40 |
| institution | Directory Open Access Journal |
| issn | 2008-2967 2251-9459 |
| language | English |
| last_indexed | 2024-04-11T15:55:14Z |
| publishDate | 2022-10-01 |
| publisher | Arak University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Toxicology |
| spelling | doaj.art-f96adfbcd89547a195ab750d8cf2cc402022-12-22T04:15:11ZengArak University of Medical SciencesIranian Journal of Toxicology2008-29672251-94592022-10-01164229236Nickel Increases Chromosomal Abnormalities by Interfering With the Initiation of DNA Repair PathwaysMahsa Ghorbani0Farhang Haddad1Khadijeh Shahrokhabadi2 Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran. Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran. Background: Nickel is a carcinogenic, heavy metal released through industrial activities and via natural resources. It is able to cause DNA damages by reducing the efficiency of DNA repair mechanisms. However, the exact time point at which it is able to interfere with these mechanisms is not yet clearly understood. Methods: To find the most nickel-vulnerable time of repair mechanisms, human dermal fibroblasts (HDF) were treated with three doses of nickel before and after X-irradiation. The induced frequency of chromosomal abnormality was studied using micronucleus assay in binucleated cells. The cytotoxicity of different treatments was established using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The results revealed that nickel treatment had a synergistic effect on inducing Micronucleus frequency only when cells were treated 2 hours before X-irradiation. The X-ray treatment of the cells with 5 and 10 mM nickel had a cytotoxic effect mainly when given 6 hours after the irradiation. Conclusion: The results suggest that nickel can interfere with human DNA repair mechanisms only at the start of the process, while having no significant effect on the human DNA repair mechanisms when activated.http://ijt.arakmu.ac.ir/article-1-1107-en.htmlchromosomesdna repair mechanismshuman dermal fibroblast (hdf)micronucleus assaynickelx-irradiation |
| spellingShingle | Mahsa Ghorbani Farhang Haddad Khadijeh Shahrokhabadi Nickel Increases Chromosomal Abnormalities by Interfering With the Initiation of DNA Repair Pathways Iranian Journal of Toxicology chromosomes dna repair mechanisms human dermal fibroblast (hdf) micronucleus assay nickel x-irradiation |
| title | Nickel Increases Chromosomal Abnormalities by Interfering With the Initiation of DNA Repair Pathways |
| title_full | Nickel Increases Chromosomal Abnormalities by Interfering With the Initiation of DNA Repair Pathways |
| title_fullStr | Nickel Increases Chromosomal Abnormalities by Interfering With the Initiation of DNA Repair Pathways |
| title_full_unstemmed | Nickel Increases Chromosomal Abnormalities by Interfering With the Initiation of DNA Repair Pathways |
| title_short | Nickel Increases Chromosomal Abnormalities by Interfering With the Initiation of DNA Repair Pathways |
| title_sort | nickel increases chromosomal abnormalities by interfering with the initiation of dna repair pathways |
| topic | chromosomes dna repair mechanisms human dermal fibroblast (hdf) micronucleus assay nickel x-irradiation |
| url | http://ijt.arakmu.ac.ir/article-1-1107-en.html |
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