| Summary: | The processes of muscle growth and development, including myoblast proliferation, migration, differentiation, and fusion, are modified by a variety of regulatory factors. <i>MYL4</i> plays an important role in atrial development, atrial cardiomyopathy, muscle-fiber size, and muscle development. The structural variation (SV) of <i>MYL4</i> was found via the de novo sequencing of Ningxiang pigs, and the existence of SV was verified in the experiments. The genotype distribution of Ningxiang pigs and Large White pigs was detected, and it was found that Ningxiang pigs were mainly of the <i>BB</i> genotype and that Large White pigs were mainly of the <i>AB</i> genotype. However, the molecular mechanisms behind the <i>MYL4</i>-mediated regulation of skeletal muscle development need to be deeply explored. Therefore, RT-qPCR, 3′RACE, CCK8, EdU, Western blot, immunofluorescence, flow cytometry, and bioinformation analysis were used to explore the function of <i>MYL4</i> in myoblast development. The cDNA of <i>MYL4</i> was successfully cloned from Ningxiang pigs, and its physicochemical properties were predicted. The expression profiles in six tissues and four stages of Ningxiang pigs and Large White pigs were found to be the highest in the lungs and 30 days after birth. The expression of <i>MYL4</i> increased gradually with the extension of the myogenic differentiation time. The myoblast function test showed that the overexpression of <i>MYL4</i> inhibited proliferation and promoted apoptosis and differentiation. The knockdown of <i>MYL4</i> showed the opposite result. These results enhance our understanding of the molecular mechanisms of muscle development and provide a solid theoretical foundation for further exploring the role of the <i>MYL4</i> gene in muscle development.
|
|---|