Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species
Coccidiosis is an intestinal disorder of poultry and often caused by simultaneous infections of several Eimeria species. GAPDH is one of the immunogenic common antigens among Eimeria tenella, E. acervulina, and E. maxima identified in our previous study. The present study was performed to further ev...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2017-07-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01245/full |
| _version_ | 1818583774942724096 |
|---|---|
| author | Lu Tian Wenyu Li Xinmei Huang Xinmei Huang Di Tian Jianhua Liu Xinchao Yang Lianrui Liu Ruofeng Yan Lixin Xu Xiangrui Li Xiaokai Song |
| author_facet | Lu Tian Wenyu Li Xinmei Huang Xinmei Huang Di Tian Jianhua Liu Xinchao Yang Lianrui Liu Ruofeng Yan Lixin Xu Xiangrui Li Xiaokai Song |
| author_sort | Lu Tian |
| collection | DOAJ |
| description | Coccidiosis is an intestinal disorder of poultry and often caused by simultaneous infections of several Eimeria species. GAPDH is one of the immunogenic common antigens among Eimeria tenella, E. acervulina, and E. maxima identified in our previous study. The present study was performed to further evaluate its immunogenicity and protective efficacy. The genes of GAPDH cloned from E. acervulina and E. maxima were named as EaGAPDH and EmGAPDH, respectively. The immunogenicity of recombinant proteins of EaGAPDH and EmGAPDH were analyzed by Western blot. The transcription and expression of pVAX-EaGAPDH and pVAX-EmGAPDH in the injected muscles were detected by reverse transcription PCR (RT-PCR) and Western blot, respectively. GAPDH-induced changes of T lymphocytes subpopulation, cytokines production, and antibody were determined using flow cytometry, quantitative real-time PCR (qPCR), and ELISA, respectively. Finally, the protective efficacies of pVAX-EaGAPDH and pVAX-EmGAPDH were evaluated by vaccination and challenge experiments. The results revealed that the recombinant GAPDH proteins reacted with the corresponding chicken antisera. The EaGAPDH genes were successfully transcribed and expressed in the injected muscles. Vaccination with pVAX-EaGAPDH and pVAX-EmGAPDH significantly increased the proportion of CD4+ and CD8+ T lymphocytes, the cytokines productions of IFN-γ, IL-2, IL-4 et al., and IgG antibody levels compared to controls. The vaccination increased the weight gains, decreased the oocyst outputs, alleviate the enteric lesions compared to controls, and induced moderate anti-coccidial index (ACI). In conclusion, the coccidial common antigen of GAPDH induced significant humoral and cellular immune response and effective protection against E. tenella, E. acervulina, E. maxima, and mixed infection of the three Eimeria species. |
| first_indexed | 2024-12-16T08:10:38Z |
| format | Article |
| id | doaj.art-f96e3cbb8edc40e1aadc93d84c6d887c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-16T08:10:38Z |
| publishDate | 2017-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f96e3cbb8edc40e1aadc93d84c6d887c2022-12-21T22:38:21ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-07-01810.3389/fmicb.2017.01245262742Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria SpeciesLu Tian0Wenyu Li1Xinmei Huang2Xinmei Huang3Di Tian4Jianhua Liu5Xinchao Yang6Lianrui Liu7Ruofeng Yan8Lixin Xu9Xiangrui Li10Xiaokai Song11College of Veterinary Medicine, Nanjing Agricultural UniversityNanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural UniversityNanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural UniversityNanjing, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural SciencesNanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural UniversityNanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural UniversityNanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural UniversityNanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural UniversityNanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural UniversityNanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural UniversityNanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural UniversityNanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural UniversityNanjing, ChinaCoccidiosis is an intestinal disorder of poultry and often caused by simultaneous infections of several Eimeria species. GAPDH is one of the immunogenic common antigens among Eimeria tenella, E. acervulina, and E. maxima identified in our previous study. The present study was performed to further evaluate its immunogenicity and protective efficacy. The genes of GAPDH cloned from E. acervulina and E. maxima were named as EaGAPDH and EmGAPDH, respectively. The immunogenicity of recombinant proteins of EaGAPDH and EmGAPDH were analyzed by Western blot. The transcription and expression of pVAX-EaGAPDH and pVAX-EmGAPDH in the injected muscles were detected by reverse transcription PCR (RT-PCR) and Western blot, respectively. GAPDH-induced changes of T lymphocytes subpopulation, cytokines production, and antibody were determined using flow cytometry, quantitative real-time PCR (qPCR), and ELISA, respectively. Finally, the protective efficacies of pVAX-EaGAPDH and pVAX-EmGAPDH were evaluated by vaccination and challenge experiments. The results revealed that the recombinant GAPDH proteins reacted with the corresponding chicken antisera. The EaGAPDH genes were successfully transcribed and expressed in the injected muscles. Vaccination with pVAX-EaGAPDH and pVAX-EmGAPDH significantly increased the proportion of CD4+ and CD8+ T lymphocytes, the cytokines productions of IFN-γ, IL-2, IL-4 et al., and IgG antibody levels compared to controls. The vaccination increased the weight gains, decreased the oocyst outputs, alleviate the enteric lesions compared to controls, and induced moderate anti-coccidial index (ACI). In conclusion, the coccidial common antigen of GAPDH induced significant humoral and cellular immune response and effective protection against E. tenella, E. acervulina, E. maxima, and mixed infection of the three Eimeria species.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01245/fullchicken coccidiancommon antigenGAPDHmixed infectionimmunogenicityprotection |
| spellingShingle | Lu Tian Wenyu Li Xinmei Huang Xinmei Huang Di Tian Jianhua Liu Xinchao Yang Lianrui Liu Ruofeng Yan Lixin Xu Xiangrui Li Xiaokai Song Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species Frontiers in Microbiology chicken coccidian common antigen GAPDH mixed infection immunogenicity protection |
| title | Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species |
| title_full | Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species |
| title_fullStr | Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species |
| title_full_unstemmed | Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species |
| title_short | Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species |
| title_sort | protective efficacy of coccidial common antigen glyceraldehyde 3 phosphate dehydrogenase gapdh against challenge with three eimeria species |
| topic | chicken coccidian common antigen GAPDH mixed infection immunogenicity protection |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01245/full |
| work_keys_str_mv | AT lutian protectiveefficacyofcoccidialcommonantigenglyceraldehyde3phosphatedehydrogenasegapdhagainstchallengewiththreeeimeriaspecies AT wenyuli protectiveefficacyofcoccidialcommonantigenglyceraldehyde3phosphatedehydrogenasegapdhagainstchallengewiththreeeimeriaspecies AT xinmeihuang protectiveefficacyofcoccidialcommonantigenglyceraldehyde3phosphatedehydrogenasegapdhagainstchallengewiththreeeimeriaspecies AT xinmeihuang protectiveefficacyofcoccidialcommonantigenglyceraldehyde3phosphatedehydrogenasegapdhagainstchallengewiththreeeimeriaspecies AT ditian protectiveefficacyofcoccidialcommonantigenglyceraldehyde3phosphatedehydrogenasegapdhagainstchallengewiththreeeimeriaspecies AT jianhualiu protectiveefficacyofcoccidialcommonantigenglyceraldehyde3phosphatedehydrogenasegapdhagainstchallengewiththreeeimeriaspecies AT xinchaoyang protectiveefficacyofcoccidialcommonantigenglyceraldehyde3phosphatedehydrogenasegapdhagainstchallengewiththreeeimeriaspecies AT lianruiliu protectiveefficacyofcoccidialcommonantigenglyceraldehyde3phosphatedehydrogenasegapdhagainstchallengewiththreeeimeriaspecies AT ruofengyan protectiveefficacyofcoccidialcommonantigenglyceraldehyde3phosphatedehydrogenasegapdhagainstchallengewiththreeeimeriaspecies AT lixinxu protectiveefficacyofcoccidialcommonantigenglyceraldehyde3phosphatedehydrogenasegapdhagainstchallengewiththreeeimeriaspecies AT xiangruili protectiveefficacyofcoccidialcommonantigenglyceraldehyde3phosphatedehydrogenasegapdhagainstchallengewiththreeeimeriaspecies AT xiaokaisong protectiveefficacyofcoccidialcommonantigenglyceraldehyde3phosphatedehydrogenasegapdhagainstchallengewiththreeeimeriaspecies |