Flexible Amperometric Immunosensor Based on Colloidal Quantum Dots for Detecting the Myeloperoxidase (MPO) Systemic Inflammation Biomarker
Myeloperoxidase (MPO) has been demonstrated to be a biomarker of neutrophilic inflammation in various diseases. Rapid detection and quantitative analysis of MPO are of great significance for human health. Herein, an MPO protein flexible amperometric immunosensor based on a colloidal quantum dot (CQD...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/2/255 |
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| author | Yanbing Tao Yunong Zhao Le Wang Jing Huang Yan Chen Qing Huang Boxiang Song Hua-Yao Li Jianjun Chen Huan Liu |
| author_facet | Yanbing Tao Yunong Zhao Le Wang Jing Huang Yan Chen Qing Huang Boxiang Song Hua-Yao Li Jianjun Chen Huan Liu |
| author_sort | Yanbing Tao |
| collection | DOAJ |
| description | Myeloperoxidase (MPO) has been demonstrated to be a biomarker of neutrophilic inflammation in various diseases. Rapid detection and quantitative analysis of MPO are of great significance for human health. Herein, an MPO protein flexible amperometric immunosensor based on a colloidal quantum dot (CQD)-modified electrode was demonstrated. The remarkable surface activity of CQDs allows them to bind directly and stably to the surface of proteins and to convert antigen–antibody specific binding reactions into significant currents. The flexible amperometric immunosensor provides quantitative analysis of MPO protein with an ultra-low limit of detection (LOD) (31.6 fg mL<sup>−1</sup>), as well as good reproducibility and stability. The detection method is expected to be applied in clinical examination, POCT (bedside test), community physical examination, home self-examination and other practical scenarios. |
| first_indexed | 2024-03-11T09:05:33Z |
| format | Article |
| id | doaj.art-f96e6bedf0a54f248b6ffc778fbc831e |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T09:05:33Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-f96e6bedf0a54f248b6ffc778fbc831e2023-11-16T19:26:18ZengMDPI AGBiosensors2079-63742023-02-0113225510.3390/bios13020255Flexible Amperometric Immunosensor Based on Colloidal Quantum Dots for Detecting the Myeloperoxidase (MPO) Systemic Inflammation BiomarkerYanbing Tao0Yunong Zhao1Le Wang2Jing Huang3Yan Chen4Qing Huang5Boxiang Song6Hua-Yao Li7Jianjun Chen8Huan Liu9School of Integrated Circuits, Wuhan National Laboratory for Optoelectronics, Optics Valley Laboratory, Huazhong University of Science and Technology, Wuhan 430074, ChinaSchool of Integrated Circuits, Wuhan National Laboratory for Optoelectronics, Optics Valley Laboratory, Huazhong University of Science and Technology, Wuhan 430074, ChinaSchool of Integrated Circuits, Wuhan National Laboratory for Optoelectronics, Optics Valley Laboratory, Huazhong University of Science and Technology, Wuhan 430074, ChinaSchool of Integrated Circuits, Wuhan National Laboratory for Optoelectronics, Optics Valley Laboratory, Huazhong University of Science and Technology, Wuhan 430074, ChinaCHINALLERGY Biotech Co., Ltd., Wuhan Institute of Biotechnology, 666 Gaoxin Road, Wuhan 430079, ChinaSchool of Integrated Circuits, Wuhan National Laboratory for Optoelectronics, Optics Valley Laboratory, Huazhong University of Science and Technology, Wuhan 430074, ChinaSchool of Integrated Circuits, Wuhan National Laboratory for Optoelectronics, Optics Valley Laboratory, Huazhong University of Science and Technology, Wuhan 430074, ChinaSchool of Integrated Circuits, Wuhan National Laboratory for Optoelectronics, Optics Valley Laboratory, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaSchool of Integrated Circuits, Wuhan National Laboratory for Optoelectronics, Optics Valley Laboratory, Huazhong University of Science and Technology, Wuhan 430074, ChinaMyeloperoxidase (MPO) has been demonstrated to be a biomarker of neutrophilic inflammation in various diseases. Rapid detection and quantitative analysis of MPO are of great significance for human health. Herein, an MPO protein flexible amperometric immunosensor based on a colloidal quantum dot (CQD)-modified electrode was demonstrated. The remarkable surface activity of CQDs allows them to bind directly and stably to the surface of proteins and to convert antigen–antibody specific binding reactions into significant currents. The flexible amperometric immunosensor provides quantitative analysis of MPO protein with an ultra-low limit of detection (LOD) (31.6 fg mL<sup>−1</sup>), as well as good reproducibility and stability. The detection method is expected to be applied in clinical examination, POCT (bedside test), community physical examination, home self-examination and other practical scenarios.https://www.mdpi.com/2079-6374/13/2/255myeloperoxidasecolloidal quantum dotflexible immunosensorinflammatory diseaseamperometric sensor |
| spellingShingle | Yanbing Tao Yunong Zhao Le Wang Jing Huang Yan Chen Qing Huang Boxiang Song Hua-Yao Li Jianjun Chen Huan Liu Flexible Amperometric Immunosensor Based on Colloidal Quantum Dots for Detecting the Myeloperoxidase (MPO) Systemic Inflammation Biomarker Biosensors myeloperoxidase colloidal quantum dot flexible immunosensor inflammatory disease amperometric sensor |
| title | Flexible Amperometric Immunosensor Based on Colloidal Quantum Dots for Detecting the Myeloperoxidase (MPO) Systemic Inflammation Biomarker |
| title_full | Flexible Amperometric Immunosensor Based on Colloidal Quantum Dots for Detecting the Myeloperoxidase (MPO) Systemic Inflammation Biomarker |
| title_fullStr | Flexible Amperometric Immunosensor Based on Colloidal Quantum Dots for Detecting the Myeloperoxidase (MPO) Systemic Inflammation Biomarker |
| title_full_unstemmed | Flexible Amperometric Immunosensor Based on Colloidal Quantum Dots for Detecting the Myeloperoxidase (MPO) Systemic Inflammation Biomarker |
| title_short | Flexible Amperometric Immunosensor Based on Colloidal Quantum Dots for Detecting the Myeloperoxidase (MPO) Systemic Inflammation Biomarker |
| title_sort | flexible amperometric immunosensor based on colloidal quantum dots for detecting the myeloperoxidase mpo systemic inflammation biomarker |
| topic | myeloperoxidase colloidal quantum dot flexible immunosensor inflammatory disease amperometric sensor |
| url | https://www.mdpi.com/2079-6374/13/2/255 |
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