Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi Infection
Phytophthora cinnamomi Rands is a cosmopolite pathogen of woody plants which during the last couple of centuries has spread all over the world from its center of origin in Southeast Asia. In contrast to Chinese cork oak (Quercus variabilis Blume) forests native to Asia, which are generally healthy d...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-06-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.894533/full |
| _version_ | 1811251381659500544 |
|---|---|
| author | Iñigo Saiz-Fernández Biljana Đorđević Pavel Kerchev Martin Černý Thomas Jung Miroslav Berka Chuen-Hsu Fu Marília Horta Jung Břetislav Brzobohatý |
| author_facet | Iñigo Saiz-Fernández Biljana Đorđević Pavel Kerchev Martin Černý Thomas Jung Miroslav Berka Chuen-Hsu Fu Marília Horta Jung Břetislav Brzobohatý |
| author_sort | Iñigo Saiz-Fernández |
| collection | DOAJ |
| description | Phytophthora cinnamomi Rands is a cosmopolite pathogen of woody plants which during the last couple of centuries has spread all over the world from its center of origin in Southeast Asia. In contrast to Chinese cork oak (Quercus variabilis Blume) forests native to Asia, which are generally healthy despite the presence of the pathogen, the populations of Cork oaks (Quercus suber L.) in Europe have been severely decimated by P. cinnamomi. The present study aims at identifying the differences in the early proteomic and metabolomic response of these two tree species that lead to their differences in susceptibility to P. cinnamomi. By using micropropagated clonal plants, we tried to minimize the plant-to-plant differences in the defense response that is maximized by the high intraspecific genetic variability inherent to the Quercus genus. The evolution on the content of Phytophthora proteins in the roots during the first 36 h after inoculation suggests a slower infection process in Q. variabilis plants. These plants displayed a significant decrease in sugars in the roots, together with a downregulation of proteins related to carbon metabolism. In the leaves, the biggest changes in proteomic profiling were observed 16 h after inoculation, and included increased abundance of peroxidases, superoxide dismutases and glutathione S-transferases in Q. variabilis plants, which probably contributed to decrease its susceptibility to P. cinnamomi. |
| first_indexed | 2024-04-12T16:18:44Z |
| format | Article |
| id | doaj.art-f9715fad82d041d69744994767b6c12d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T16:18:44Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f9715fad82d041d69744994767b6c12d2022-12-22T03:25:38ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.894533894533Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi InfectionIñigo Saiz-Fernández0Biljana Đorđević1Pavel Kerchev2Martin Černý3Thomas Jung4Miroslav Berka5Chuen-Hsu Fu6Marília Horta Jung7Břetislav Brzobohatý8Department of Molecular Biology and Radiobiology, Faculty of AgriSciences, Phytophthora Research Centre, Mendel University in Brno, Brno, CzechiaDepartment of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Phytophthora Research Centre, Mendel University in Brno, Brno, CzechiaDepartment of Molecular Biology and Radiobiology, Faculty of AgriSciences, Phytophthora Research Centre, Mendel University in Brno, Brno, CzechiaDepartment of Molecular Biology and Radiobiology, Faculty of AgriSciences, Phytophthora Research Centre, Mendel University in Brno, Brno, CzechiaDepartment of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Phytophthora Research Centre, Mendel University in Brno, Brno, CzechiaDepartment of Molecular Biology and Radiobiology, Faculty of AgriSciences, Phytophthora Research Centre, Mendel University in Brno, Brno, CzechiaForest Protection Division, Taiwan Forestry Research Institute, Taipei, TaiwanDepartment of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Phytophthora Research Centre, Mendel University in Brno, Brno, CzechiaDepartment of Molecular Biology and Radiobiology, Faculty of AgriSciences, Phytophthora Research Centre, Mendel University in Brno, Brno, CzechiaPhytophthora cinnamomi Rands is a cosmopolite pathogen of woody plants which during the last couple of centuries has spread all over the world from its center of origin in Southeast Asia. In contrast to Chinese cork oak (Quercus variabilis Blume) forests native to Asia, which are generally healthy despite the presence of the pathogen, the populations of Cork oaks (Quercus suber L.) in Europe have been severely decimated by P. cinnamomi. The present study aims at identifying the differences in the early proteomic and metabolomic response of these two tree species that lead to their differences in susceptibility to P. cinnamomi. By using micropropagated clonal plants, we tried to minimize the plant-to-plant differences in the defense response that is maximized by the high intraspecific genetic variability inherent to the Quercus genus. The evolution on the content of Phytophthora proteins in the roots during the first 36 h after inoculation suggests a slower infection process in Q. variabilis plants. These plants displayed a significant decrease in sugars in the roots, together with a downregulation of proteins related to carbon metabolism. In the leaves, the biggest changes in proteomic profiling were observed 16 h after inoculation, and included increased abundance of peroxidases, superoxide dismutases and glutathione S-transferases in Q. variabilis plants, which probably contributed to decrease its susceptibility to P. cinnamomi.https://www.frontiersin.org/articles/10.3389/fmicb.2022.894533/fullmicropropagationplant pathogenresistancesugarsperoxidasesglutathione S-transferase |
| spellingShingle | Iñigo Saiz-Fernández Biljana Đorđević Pavel Kerchev Martin Černý Thomas Jung Miroslav Berka Chuen-Hsu Fu Marília Horta Jung Břetislav Brzobohatý Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi Infection Frontiers in Microbiology micropropagation plant pathogen resistance sugars peroxidases glutathione S-transferase |
| title | Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi Infection |
| title_full | Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi Infection |
| title_fullStr | Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi Infection |
| title_full_unstemmed | Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi Infection |
| title_short | Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi Infection |
| title_sort | differences in the proteomic and metabolomic response of quercus suber and quercus variabilis during the early stages of phytophthora cinnamomi infection |
| topic | micropropagation plant pathogen resistance sugars peroxidases glutathione S-transferase |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.894533/full |
| work_keys_str_mv | AT inigosaizfernandez differencesintheproteomicandmetabolomicresponseofquercussuberandquercusvariabilisduringtheearlystagesofphytophthoracinnamomiinfection AT biljanađorđevic differencesintheproteomicandmetabolomicresponseofquercussuberandquercusvariabilisduringtheearlystagesofphytophthoracinnamomiinfection AT pavelkerchev differencesintheproteomicandmetabolomicresponseofquercussuberandquercusvariabilisduringtheearlystagesofphytophthoracinnamomiinfection AT martincerny differencesintheproteomicandmetabolomicresponseofquercussuberandquercusvariabilisduringtheearlystagesofphytophthoracinnamomiinfection AT thomasjung differencesintheproteomicandmetabolomicresponseofquercussuberandquercusvariabilisduringtheearlystagesofphytophthoracinnamomiinfection AT miroslavberka differencesintheproteomicandmetabolomicresponseofquercussuberandquercusvariabilisduringtheearlystagesofphytophthoracinnamomiinfection AT chuenhsufu differencesintheproteomicandmetabolomicresponseofquercussuberandquercusvariabilisduringtheearlystagesofphytophthoracinnamomiinfection AT mariliahortajung differencesintheproteomicandmetabolomicresponseofquercussuberandquercusvariabilisduringtheearlystagesofphytophthoracinnamomiinfection AT bretislavbrzobohaty differencesintheproteomicandmetabolomicresponseofquercussuberandquercusvariabilisduringtheearlystagesofphytophthoracinnamomiinfection |