Characterization of phospholipase B of Culex pipiens fatigans
Phospholipase B has been found in the mosquito Culex pipiens fatigans, and some of its properties have been studied. The enzyme had a high optimum temperature (45°C) and broad alkaline pH optimum (8-9). It was inactive toward diacylphospholipids, and hydrolyzed lysolecithin at a higher rate than lys...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1969-11-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520430238 |
| _version_ | 1819055312961798144 |
|---|---|
| author | R. Hanumantha Rao D. Subrahmanyam |
| author_facet | R. Hanumantha Rao D. Subrahmanyam |
| author_sort | R. Hanumantha Rao |
| collection | DOAJ |
| description | Phospholipase B has been found in the mosquito Culex pipiens fatigans, and some of its properties have been studied. The enzyme had a high optimum temperature (45°C) and broad alkaline pH optimum (8-9). It was inactive toward diacylphospholipids, and hydrolyzed lysolecithin at a higher rate than lysophosphatidyl ethanolamine. The enzyme was heat labile, but lysolecithin protected it against heat inactivation. Phosphatidyl ethanolamine, phosphatidyl choline, deoxycholate, Fe+++, and Hg++ inhibited the enzyme markedly.The enzyme was present mainly in larvae; little enzyme activity was detected in pupae or adults. The total and specific activities were highest in IV instar (6 day) and I instar (1st day) larvae, respectively. It was localized in the microsomal fraction and was distributed mainly in the abdomen and thorax of the insect. The enzyme was present at much higher levels of activity in larvae of the mosquitoes Anopheles stephensi and Aedes aegypti. |
| first_indexed | 2024-12-21T13:05:32Z |
| format | Article |
| id | doaj.art-f974cb8b5c9b492db108c03eae1ee02f |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T13:05:32Z |
| publishDate | 1969-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-f974cb8b5c9b492db108c03eae1ee02f2022-12-21T19:03:02ZengElsevierJournal of Lipid Research0022-22751969-11-01106636641Characterization of phospholipase B of Culex pipiens fatigansR. Hanumantha Rao0D. Subrahmanyam1Biochemistry Division, National Institute of Communicable Diseases, Delhi, IndiaBiochemistry Division, National Institute of Communicable Diseases, Delhi, IndiaPhospholipase B has been found in the mosquito Culex pipiens fatigans, and some of its properties have been studied. The enzyme had a high optimum temperature (45°C) and broad alkaline pH optimum (8-9). It was inactive toward diacylphospholipids, and hydrolyzed lysolecithin at a higher rate than lysophosphatidyl ethanolamine. The enzyme was heat labile, but lysolecithin protected it against heat inactivation. Phosphatidyl ethanolamine, phosphatidyl choline, deoxycholate, Fe+++, and Hg++ inhibited the enzyme markedly.The enzyme was present mainly in larvae; little enzyme activity was detected in pupae or adults. The total and specific activities were highest in IV instar (6 day) and I instar (1st day) larvae, respectively. It was localized in the microsomal fraction and was distributed mainly in the abdomen and thorax of the insect. The enzyme was present at much higher levels of activity in larvae of the mosquitoes Anopheles stephensi and Aedes aegypti.http://www.sciencedirect.com/science/article/pii/S0022227520430238mosquito vectorsdevelopmental stageslysophospholipidsalkaline pH optimumhigh optimum temperaturedeoxycholate |
| spellingShingle | R. Hanumantha Rao D. Subrahmanyam Characterization of phospholipase B of Culex pipiens fatigans Journal of Lipid Research mosquito vectors developmental stages lysophospholipids alkaline pH optimum high optimum temperature deoxycholate |
| title | Characterization of phospholipase B of Culex pipiens fatigans |
| title_full | Characterization of phospholipase B of Culex pipiens fatigans |
| title_fullStr | Characterization of phospholipase B of Culex pipiens fatigans |
| title_full_unstemmed | Characterization of phospholipase B of Culex pipiens fatigans |
| title_short | Characterization of phospholipase B of Culex pipiens fatigans |
| title_sort | characterization of phospholipase b of culex pipiens fatigans |
| topic | mosquito vectors developmental stages lysophospholipids alkaline pH optimum high optimum temperature deoxycholate |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520430238 |
| work_keys_str_mv | AT rhanumantharao characterizationofphospholipasebofculexpipiensfatigans AT dsubrahmanyam characterizationofphospholipasebofculexpipiensfatigans |