Point-of-Care PCR Assays for COVID-19 Detection
Molecular diagnostics has been the front runner in the world’s response to the COVID-19 pandemic. Particularly, reverse transcriptase-polymerase chain reaction (RT-PCR) and the quantitative variant (qRT-PCR) have been the gold standard for COVID-19 diagnosis. However, faster antigen tests and other...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/5/141 |
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| author | Niharika Gupta Shine Augustine Tarun Narayan Alan O’Riordan Asmita Das D. Kumar John H. T. Luong Bansi D. Malhotra |
| author_facet | Niharika Gupta Shine Augustine Tarun Narayan Alan O’Riordan Asmita Das D. Kumar John H. T. Luong Bansi D. Malhotra |
| author_sort | Niharika Gupta |
| collection | DOAJ |
| description | Molecular diagnostics has been the front runner in the world’s response to the COVID-19 pandemic. Particularly, reverse transcriptase-polymerase chain reaction (RT-PCR) and the quantitative variant (qRT-PCR) have been the gold standard for COVID-19 diagnosis. However, faster antigen tests and other point-of-care (POC) devices have also played a significant role in containing the spread of SARS-CoV-2 by facilitating mass screening and delivering results in less time. Thus, despite the higher sensitivity and specificity of the RT-PCR assays, the impact of POC tests cannot be ignored. As a consequence, there has been an increased interest in the development of miniaturized, high-throughput, and automated PCR systems, many of which can be used at point-of-care. This review summarizes the recent advances in the development of miniaturized PCR systems with an emphasis on COVID-19 detection. The distinct features of digital PCR and electrochemical PCR are detailed along with the challenges. The potential of CRISPR/Cas technology for POC diagnostics is also highlighted. Commercial RT–PCR POC systems approved by various agencies for COVID-19 detection are discussed. |
| first_indexed | 2024-03-10T11:45:49Z |
| format | Article |
| id | doaj.art-f974efce97094c34b2677b68d9d21f9b |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T11:45:49Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-f974efce97094c34b2677b68d9d21f9b2023-11-21T18:08:16ZengMDPI AGBiosensors2079-63742021-05-0111514110.3390/bios11050141Point-of-Care PCR Assays for COVID-19 DetectionNiharika Gupta0Shine Augustine1Tarun Narayan2Alan O’Riordan3Asmita Das4D. Kumar5John H. T. Luong6Bansi D. Malhotra7Department of Biotechnology, Delhi Technological University, Shahbad Daulatpur, Delhi 110042, IndiaDepartment of Biotechnology, Delhi Technological University, Shahbad Daulatpur, Delhi 110042, IndiaNanotechnology Group, Tyndall National Institute, University College Cork, T12 K8AF Cork, IrelandNanotechnology Group, Tyndall National Institute, University College Cork, T12 K8AF Cork, IrelandDepartment of Biotechnology, Delhi Technological University, Shahbad Daulatpur, Delhi 110042, IndiaDepartment of Applied Chemistry, Delhi Technological University, Shahbad Daulatpur, New Delhi 110042, IndiaSchool of Chemistry, University College Cork, T12 K8AF Cork, IrelandDepartment of Biotechnology, Delhi Technological University, Shahbad Daulatpur, Delhi 110042, IndiaMolecular diagnostics has been the front runner in the world’s response to the COVID-19 pandemic. Particularly, reverse transcriptase-polymerase chain reaction (RT-PCR) and the quantitative variant (qRT-PCR) have been the gold standard for COVID-19 diagnosis. However, faster antigen tests and other point-of-care (POC) devices have also played a significant role in containing the spread of SARS-CoV-2 by facilitating mass screening and delivering results in less time. Thus, despite the higher sensitivity and specificity of the RT-PCR assays, the impact of POC tests cannot be ignored. As a consequence, there has been an increased interest in the development of miniaturized, high-throughput, and automated PCR systems, many of which can be used at point-of-care. This review summarizes the recent advances in the development of miniaturized PCR systems with an emphasis on COVID-19 detection. The distinct features of digital PCR and electrochemical PCR are detailed along with the challenges. The potential of CRISPR/Cas technology for POC diagnostics is also highlighted. Commercial RT–PCR POC systems approved by various agencies for COVID-19 detection are discussed.https://www.mdpi.com/2079-6374/11/5/141polymerase chain reactionCOVID-19electrochemicaldigital PCRpoint-of-care |
| spellingShingle | Niharika Gupta Shine Augustine Tarun Narayan Alan O’Riordan Asmita Das D. Kumar John H. T. Luong Bansi D. Malhotra Point-of-Care PCR Assays for COVID-19 Detection Biosensors polymerase chain reaction COVID-19 electrochemical digital PCR point-of-care |
| title | Point-of-Care PCR Assays for COVID-19 Detection |
| title_full | Point-of-Care PCR Assays for COVID-19 Detection |
| title_fullStr | Point-of-Care PCR Assays for COVID-19 Detection |
| title_full_unstemmed | Point-of-Care PCR Assays for COVID-19 Detection |
| title_short | Point-of-Care PCR Assays for COVID-19 Detection |
| title_sort | point of care pcr assays for covid 19 detection |
| topic | polymerase chain reaction COVID-19 electrochemical digital PCR point-of-care |
| url | https://www.mdpi.com/2079-6374/11/5/141 |
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