Improvement in The Function of Isolated Rat Pancreatic Islets through Reduction of Oxidative Stress Using Traditional Iranian Medicine
Objective: Pancreatic islets have fewer antioxidant enzymes than other tissues and thus are vulnerable to oxidative stress. In the present study, the effects of nine specifically selected Iranian medical plants on the mitochondria function and survival of isolated rat islets were examined. Material...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2014-06-01
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| Series: | Cell Journal |
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| Online Access: | http://celljournal.org/library/upload/article/af_73432422444775633542442772742644525464266-Mahban%20Rahimifard-1.pdf |
| _version_ | 1831645414552502272 |
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| author | Mahban Rahimifard Mona Navaei-Nigjeh Neda Mahroui Sanaz Mirzaei Zahra Siahpoosh Amir Nili-Ahmadabadi Azadeh Mohammadirad Maryam Baeeri Reza Hajiaghaie Mohammad Abdollahi |
| author_facet | Mahban Rahimifard Mona Navaei-Nigjeh Neda Mahroui Sanaz Mirzaei Zahra Siahpoosh Amir Nili-Ahmadabadi Azadeh Mohammadirad Maryam Baeeri Reza Hajiaghaie Mohammad Abdollahi |
| author_sort | Mahban Rahimifard |
| collection | DOAJ |
| description | Objective: Pancreatic islets have fewer antioxidant enzymes than other tissues and thus are vulnerable to oxidative stress. In the present study, the effects of nine specifically selected Iranian medical plants on the mitochondria function and survival of isolated rat islets were examined.
Materials and Methods: In this experimental study, following laparotomy, pancreases of rats were removed and the islets isolated and incubated in vitro for 24 hours. Logarithmic doses of plant materials were added to the islets and incubated for an additional 24 hours after which the viability of the cells and production of reactive oxygen species (ROS) were measured. Levels of insulin production in relation to static and stimulated glucose concentrations were also determined.
Results: The tested compounds markedly increased survival of the islet cells, their mitochondrial activity, and insulin levels at the same time as reducing production of ROS. Greatest effects were observed in the following order: Peganum harmala, Glycyrrhiza glabra, Satureja hortensis, Rosmarinus officinalis, Teucrium scordium, Aloe vera, Zingiber officinale, Silybum marianum, and Hypericum perforatum at doses of 10, 103, 104, 10, 102, 102, 10-1, 10 and 103 μgmL-1, respectively.
Conclusion: Based on these results, we suggest that pretreatment with these selected Iranian medical plants can improve the outcomes of pancreas transplants and grafts through the control of oxidative stress damage. |
| first_indexed | 2024-12-19T13:36:12Z |
| format | Article |
| id | doaj.art-f97731fa90994a19b8d4c64cfc9cd0b4 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-19T13:36:12Z |
| publishDate | 2014-06-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-f97731fa90994a19b8d4c64cfc9cd0b42022-12-21T20:19:12ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142014-06-01162147162Improvement in The Function of Isolated Rat Pancreatic Islets through Reduction of Oxidative Stress Using Traditional Iranian MedicineMahban Rahimifard 0 Mona Navaei-Nigjeh 1 Neda Mahroui 2Sanaz Mirzaei 3 Zahra Siahpoosh 4 Amir Nili-Ahmadabadi 5Azadeh Mohammadirad6 Maryam Baeeri 7Reza Hajiaghaie 8Mohammad Abdollahi 9Faculty of Science and Agriculture, Payame Noor University of Tehran, Tehran, IranFaculty of Pharmacy and Pharmaceutical Sciences Research Centre, Tehran University of Medical Sciences,Tehran, IranPharmaceutical Branch, Islamic Azad University of Medical Sciences, Tehran, IranPharmaceutical Branch, Islamic Azad University of Medical Sciences, Tehran, IranPharmaceutical Branch, Islamic Azad University of Medical Sciences, Tehran, IranFaculty of Pharmacy and Pharmaceutical Sciences Research Centre, Tehran University of Medical Sciences,Tehran, IranFaculty of Pharmacy and Pharmaceutical Sciences Research Centre, Tehran University of Medical Sciences,Tehran, IranFaculty of Pharmacy and Pharmaceutical Sciences Research Centre, Tehran University of Medical Sciences,Tehran, IranPharmacognosy and Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, IranFaculty of Pharmacy and Pharmaceutical Sciences Research Centre, Tehran University of Medical Sciences,Tehran, IranObjective: Pancreatic islets have fewer antioxidant enzymes than other tissues and thus are vulnerable to oxidative stress. In the present study, the effects of nine specifically selected Iranian medical plants on the mitochondria function and survival of isolated rat islets were examined. Materials and Methods: In this experimental study, following laparotomy, pancreases of rats were removed and the islets isolated and incubated in vitro for 24 hours. Logarithmic doses of plant materials were added to the islets and incubated for an additional 24 hours after which the viability of the cells and production of reactive oxygen species (ROS) were measured. Levels of insulin production in relation to static and stimulated glucose concentrations were also determined. Results: The tested compounds markedly increased survival of the islet cells, their mitochondrial activity, and insulin levels at the same time as reducing production of ROS. Greatest effects were observed in the following order: Peganum harmala, Glycyrrhiza glabra, Satureja hortensis, Rosmarinus officinalis, Teucrium scordium, Aloe vera, Zingiber officinale, Silybum marianum, and Hypericum perforatum at doses of 10, 103, 104, 10, 102, 102, 10-1, 10 and 103 μgmL-1, respectively. Conclusion: Based on these results, we suggest that pretreatment with these selected Iranian medical plants can improve the outcomes of pancreas transplants and grafts through the control of oxidative stress damage.http://celljournal.org/library/upload/article/af_73432422444775633542442772742644525464266-Mahban%20Rahimifard-1.pdfIslets of LangerhansOxidative StressMedicineIranian TraditionalIn Vitro |
| spellingShingle | Mahban Rahimifard Mona Navaei-Nigjeh Neda Mahroui Sanaz Mirzaei Zahra Siahpoosh Amir Nili-Ahmadabadi Azadeh Mohammadirad Maryam Baeeri Reza Hajiaghaie Mohammad Abdollahi Improvement in The Function of Isolated Rat Pancreatic Islets through Reduction of Oxidative Stress Using Traditional Iranian Medicine Cell Journal Islets of Langerhans Oxidative Stress Medicine Iranian Traditional In Vitro |
| title | Improvement in The Function of Isolated Rat Pancreatic Islets through Reduction of Oxidative Stress Using Traditional Iranian Medicine |
| title_full | Improvement in The Function of Isolated Rat Pancreatic Islets through Reduction of Oxidative Stress Using Traditional Iranian Medicine |
| title_fullStr | Improvement in The Function of Isolated Rat Pancreatic Islets through Reduction of Oxidative Stress Using Traditional Iranian Medicine |
| title_full_unstemmed | Improvement in The Function of Isolated Rat Pancreatic Islets through Reduction of Oxidative Stress Using Traditional Iranian Medicine |
| title_short | Improvement in The Function of Isolated Rat Pancreatic Islets through Reduction of Oxidative Stress Using Traditional Iranian Medicine |
| title_sort | improvement in the function of isolated rat pancreatic islets through reduction of oxidative stress using traditional iranian medicine |
| topic | Islets of Langerhans Oxidative Stress Medicine Iranian Traditional In Vitro |
| url | http://celljournal.org/library/upload/article/af_73432422444775633542442772742644525464266-Mahban%20Rahimifard-1.pdf |
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