Crohn's Disease: Retrospective Study In Algerian Patients
Background: Inflammatory disease of Crohn affects the entire digestive tract, with extra-intestinal manifestations and immune disorders. Objectives: This work aims to represent the histopathological aspects of Crohn‘s disease and the establishment of pathogenic bacteria as causal agents. Meth...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Jinnah University for Women
2023-02-01
|
| Series: | RADS Journal of Biological Research & Applied Science |
| Subjects: | |
| Online Access: | http://jbas.juw.edu.pk/index.php/JBAS/article/view/565 |
| _version_ | 1797893886024089600 |
|---|---|
| author | Hassina Guetarni Meriem Aissou |
| author_facet | Hassina Guetarni Meriem Aissou |
| author_sort | Hassina Guetarni |
| collection | DOAJ |
| description |
Background: Inflammatory disease of Crohn affects the entire digestive tract, with extra-intestinal manifestations and immune disorders.
Objectives: This work aims to represent the histopathological aspects of Crohn‘s disease and the establishment of pathogenic bacteria as causal agents.
Methodology: The histopathological aspects of the disease were studied on a colonic resection specimen and on intestinal biopsies with colorations topographic staining. Pathogenic bacteria responsible for Crohn‘s disease have also been isolated and identified. The study is continued to establish a correlation between the disease and exposure to infections by unusual bacteria, particularly pathogens (Salmonella, Shigella, Streptococcus pyogenes, Klebsiella).
Results : The macroscopic appearance of the disease presented transmural involvement and can be complicated by abscesses and fistulas, microscopic appearance indicated the infiltrate of inflammatory cells (lymphocytes, plasma cells) and lymphoid follicles after topographic staining.
Crohn’s disease is an idiopathic disease, it is assumed that it is a deregulation of the immune system due to an infectious agent in genetically predisposed people.
In our work, we studied the microbiota of intestine and stool of Crohn’s patients in which we found certain bacteria including Proteus mirabilis with a predominance of E. coli. Other pathogenic bacteria were found like Salmonella spp., Shigella spp., Klebsiella spp. and Streptococcus pyogenes. Two cases which tested positive on Ziehl Neelsen stain represented Mycobacterium avium paratuberculosis.
Conclusion: The histopathological aspect of CD can be better visualized and identified on surgical specimens than on endoscopic biopsies, which helps to monitor the evolution of the disease and must be accompanied by clinical, serological and radiological exploration.
|
| first_indexed | 2024-04-10T07:00:02Z |
| format | Article |
| id | doaj.art-f9779b9b8f4d42279ec884c86199876b |
| institution | Directory Open Access Journal |
| issn | 2305-8722 2521-8573 |
| language | English |
| last_indexed | 2024-04-10T07:00:02Z |
| publishDate | 2023-02-01 |
| publisher | Jinnah University for Women |
| record_format | Article |
| series | RADS Journal of Biological Research & Applied Science |
| spelling | doaj.art-f9779b9b8f4d42279ec884c86199876b2023-02-28T02:53:17ZengJinnah University for WomenRADS Journal of Biological Research & Applied Science2305-87222521-85732023-02-0113210.37962/jbas.v13i2.565Crohn's Disease: Retrospective Study In Algerian PatientsHassina Guetarni0Meriem Aissou1Faculty of Natural Sciences, University Bounaama Djilali of Khemis Miliana, Ain Defla, Algeria.Faculty of Natural Sciences, University Bounaama Djilali of Khemis Miliana, Ain Defla, Algeria. Background: Inflammatory disease of Crohn affects the entire digestive tract, with extra-intestinal manifestations and immune disorders. Objectives: This work aims to represent the histopathological aspects of Crohn‘s disease and the establishment of pathogenic bacteria as causal agents. Methodology: The histopathological aspects of the disease were studied on a colonic resection specimen and on intestinal biopsies with colorations topographic staining. Pathogenic bacteria responsible for Crohn‘s disease have also been isolated and identified. The study is continued to establish a correlation between the disease and exposure to infections by unusual bacteria, particularly pathogens (Salmonella, Shigella, Streptococcus pyogenes, Klebsiella). Results : The macroscopic appearance of the disease presented transmural involvement and can be complicated by abscesses and fistulas, microscopic appearance indicated the infiltrate of inflammatory cells (lymphocytes, plasma cells) and lymphoid follicles after topographic staining. Crohn’s disease is an idiopathic disease, it is assumed that it is a deregulation of the immune system due to an infectious agent in genetically predisposed people. In our work, we studied the microbiota of intestine and stool of Crohn’s patients in which we found certain bacteria including Proteus mirabilis with a predominance of E. coli. Other pathogenic bacteria were found like Salmonella spp., Shigella spp., Klebsiella spp. and Streptococcus pyogenes. Two cases which tested positive on Ziehl Neelsen stain represented Mycobacterium avium paratuberculosis. Conclusion: The histopathological aspect of CD can be better visualized and identified on surgical specimens than on endoscopic biopsies, which helps to monitor the evolution of the disease and must be accompanied by clinical, serological and radiological exploration. http://jbas.juw.edu.pk/index.php/JBAS/article/view/565Crohn disease, inflammatory granulomas, lymphoid follicle, Ziehl Neelsen staining, Hematoxylin eosin staining, Mycobacterium avium paratuberculosis. |
| spellingShingle | Hassina Guetarni Meriem Aissou Crohn's Disease: Retrospective Study In Algerian Patients RADS Journal of Biological Research & Applied Science Crohn disease, inflammatory granulomas, lymphoid follicle, Ziehl Neelsen staining, Hematoxylin eosin staining, Mycobacterium avium paratuberculosis. |
| title | Crohn's Disease: Retrospective Study In Algerian Patients |
| title_full | Crohn's Disease: Retrospective Study In Algerian Patients |
| title_fullStr | Crohn's Disease: Retrospective Study In Algerian Patients |
| title_full_unstemmed | Crohn's Disease: Retrospective Study In Algerian Patients |
| title_short | Crohn's Disease: Retrospective Study In Algerian Patients |
| title_sort | crohn s disease retrospective study in algerian patients |
| topic | Crohn disease, inflammatory granulomas, lymphoid follicle, Ziehl Neelsen staining, Hematoxylin eosin staining, Mycobacterium avium paratuberculosis. |
| url | http://jbas.juw.edu.pk/index.php/JBAS/article/view/565 |
| work_keys_str_mv | AT hassinaguetarni crohnsdiseaseretrospectivestudyinalgerianpatients AT meriemaissou crohnsdiseaseretrospectivestudyinalgerianpatients |