<i>Bacillus subtilis</i> Protects the Ducks from Oxidative Stress Induced by <i>Escherichia coli</i>: Efficacy and Molecular Mechanism
<i>Bacillus subtilis</i> has been widely used in animal husbandry as a potential alternative to antibiotics due to its excellent bacteriostasis and antioxidant activity. This study aims to investigate the effects of <i>Bacillus subtilis</i> on the protection of ducks from <...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/10/1951 |
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| author | Chong Li Yang Li Shuzhen Li Si Chen Guohua Liu Xuejuan Deng Wenhuan Chang Huiyi Cai |
| author_facet | Chong Li Yang Li Shuzhen Li Si Chen Guohua Liu Xuejuan Deng Wenhuan Chang Huiyi Cai |
| author_sort | Chong Li |
| collection | DOAJ |
| description | <i>Bacillus subtilis</i> has been widely used in animal husbandry as a potential alternative to antibiotics due to its excellent bacteriostasis and antioxidant activity. This study aims to investigate the effects of <i>Bacillus subtilis</i> on the protection of ducks from <i>Escherichia coli</i> infection and its mechanism. The four experimental groups include the negative control group, positive control group, antibiotic group and <i>Bacillus subtilis</i> group. Ducks in positive, antibiotic and <i>Bacillus subtilis</i> groups are orally administered with <i>Escherichia coli</i> and equivalent saline solution for the negative group. The results show that supplements with <i>Bacillus subtilis</i> enhances the performance and health status of the infected ducks. Moreover, <i>Bacillus subtilis</i> alleviates the increase in globulin, LPS and MDA, and the decrease in albumin, T-AOC and T-SOD in the serum caused by <i>Escherichia coli</i> infection. <i>Bacillus subtilis</i> also attenuates injury in the intestine and partially reverses the increase in ROS production and the depletion of ATP in the jejunum. These effects are accompanied with the change of related genes of the ribosome (13.54%) and oxidative phosphorylation (6.68%). Collectively, <i>Bacillus subtilis</i> alleviates the damage caused by <i>Escherichia coli</i> infection in ducks by activating ribosome and oxidative phosphorylation signaling to regulate antioxidant and energy metabolism. |
| first_indexed | 2024-03-09T20:50:07Z |
| format | Article |
| id | doaj.art-f97b21e921f443ea9712cd82a1fd2eb7 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T20:50:07Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-f97b21e921f443ea9712cd82a1fd2eb72023-11-23T22:38:22ZengMDPI AGAntioxidants2076-39212022-09-011110195110.3390/antiox11101951<i>Bacillus subtilis</i> Protects the Ducks from Oxidative Stress Induced by <i>Escherichia coli</i>: Efficacy and Molecular MechanismChong Li0Yang Li1Shuzhen Li2Si Chen3Guohua Liu4Xuejuan Deng5Wenhuan Chang6Huiyi Cai7Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaDepartment of Molecular Cell Biology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Suwon 16419, KoreaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaNational Engineering Research Center of Biological Feed, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, China<i>Bacillus subtilis</i> has been widely used in animal husbandry as a potential alternative to antibiotics due to its excellent bacteriostasis and antioxidant activity. This study aims to investigate the effects of <i>Bacillus subtilis</i> on the protection of ducks from <i>Escherichia coli</i> infection and its mechanism. The four experimental groups include the negative control group, positive control group, antibiotic group and <i>Bacillus subtilis</i> group. Ducks in positive, antibiotic and <i>Bacillus subtilis</i> groups are orally administered with <i>Escherichia coli</i> and equivalent saline solution for the negative group. The results show that supplements with <i>Bacillus subtilis</i> enhances the performance and health status of the infected ducks. Moreover, <i>Bacillus subtilis</i> alleviates the increase in globulin, LPS and MDA, and the decrease in albumin, T-AOC and T-SOD in the serum caused by <i>Escherichia coli</i> infection. <i>Bacillus subtilis</i> also attenuates injury in the intestine and partially reverses the increase in ROS production and the depletion of ATP in the jejunum. These effects are accompanied with the change of related genes of the ribosome (13.54%) and oxidative phosphorylation (6.68%). Collectively, <i>Bacillus subtilis</i> alleviates the damage caused by <i>Escherichia coli</i> infection in ducks by activating ribosome and oxidative phosphorylation signaling to regulate antioxidant and energy metabolism.https://www.mdpi.com/2076-3921/11/10/1951<i>Bacillus subtilis</i>pekin duck<i>Escherichia coli</i>oxidative stressribosomeoxidative phosphorylation |
| spellingShingle | Chong Li Yang Li Shuzhen Li Si Chen Guohua Liu Xuejuan Deng Wenhuan Chang Huiyi Cai <i>Bacillus subtilis</i> Protects the Ducks from Oxidative Stress Induced by <i>Escherichia coli</i>: Efficacy and Molecular Mechanism Antioxidants <i>Bacillus subtilis</i> pekin duck <i>Escherichia coli</i> oxidative stress ribosome oxidative phosphorylation |
| title | <i>Bacillus subtilis</i> Protects the Ducks from Oxidative Stress Induced by <i>Escherichia coli</i>: Efficacy and Molecular Mechanism |
| title_full | <i>Bacillus subtilis</i> Protects the Ducks from Oxidative Stress Induced by <i>Escherichia coli</i>: Efficacy and Molecular Mechanism |
| title_fullStr | <i>Bacillus subtilis</i> Protects the Ducks from Oxidative Stress Induced by <i>Escherichia coli</i>: Efficacy and Molecular Mechanism |
| title_full_unstemmed | <i>Bacillus subtilis</i> Protects the Ducks from Oxidative Stress Induced by <i>Escherichia coli</i>: Efficacy and Molecular Mechanism |
| title_short | <i>Bacillus subtilis</i> Protects the Ducks from Oxidative Stress Induced by <i>Escherichia coli</i>: Efficacy and Molecular Mechanism |
| title_sort | i bacillus subtilis i protects the ducks from oxidative stress induced by i escherichia coli i efficacy and molecular mechanism |
| topic | <i>Bacillus subtilis</i> pekin duck <i>Escherichia coli</i> oxidative stress ribosome oxidative phosphorylation |
| url | https://www.mdpi.com/2076-3921/11/10/1951 |
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