Growth and Biochemical Responses of Potato Cultivars under In Vitro Lithium Chloride and Mannitol Simulated Salinity and Drought Stress
Globally, drought and salinity stress critically constrain potato (<i>Solanum tuberosum</i> L.) production. Considering the impact of these stresses on crops and increasing food demand, insight into both tolerance and susceptibility is essential. The present study screens two potato cult...
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MDPI AG
2021-05-01
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| Series: | Plants |
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| author | Farooq Abdul Sattar Bahget Talat Hamooh Gordon Wellman Md. Arfan Ali Saad Hussain Shah Yasir Anwar Magdi Ali Ahmed Mousa |
| author_facet | Farooq Abdul Sattar Bahget Talat Hamooh Gordon Wellman Md. Arfan Ali Saad Hussain Shah Yasir Anwar Magdi Ali Ahmed Mousa |
| author_sort | Farooq Abdul Sattar |
| collection | DOAJ |
| description | Globally, drought and salinity stress critically constrain potato (<i>Solanum tuberosum</i> L.) production. Considering the impact of these stresses on crops and increasing food demand, insight into both tolerance and susceptibility is essential. The present study screens two potato cultivars, BARI-401 and Spunta, for their tolerance to simulated salinity and drought by in vitro LiCl and mannitol exposure. Plantlets treated with a range of LiCl (0, 10, 30, and 40 mM) and mannitol (0, 50, 100, 200, and 250 mM) concentrations were biochemically and physiologically characterized to assess their tolerance capacity. Shoot number, shoot length, root number, and root length were affected in both cultivars under higher LiCl and mannitol concentrations, even though Spunta was able to better maintain a higher shoot length under the 40 mM of LiCl and 250 mM of mannitol compared to BARI-401. The total phenol contents (TPC) in both cultivars were increased at the highest treatment concentration and the total flavonoids content (TFC) was decreased in BARI-401 as compared to Spunta. Higher free radical scavenging capacity (FRSC, low IC<sub>50</sub> value) was recorded in Spunta as compared to BARI-401 with increasing treatment concentrations, which supports the high antioxidant capacity of Spunta. An inverse correlation between polyphenol oxidase (PPO) and TPC was noted in both cultivars. Peroxidase dismutase (POD) activity was increased significantly in both cultivars for all treatments, but activity was highest overall in Spunta. These physiological and biochemical analyses of both cultivars suggest that cultivar Spunta is more tolerant to salinity and drought stress. Further open-field experiments are required to confirm these results. |
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| spelling | doaj.art-f97fa4902560474388a7e68f4f2520ab2023-11-21T18:29:23ZengMDPI AGPlants2223-77472021-05-0110592410.3390/plants10050924Growth and Biochemical Responses of Potato Cultivars under In Vitro Lithium Chloride and Mannitol Simulated Salinity and Drought StressFarooq Abdul Sattar0Bahget Talat Hamooh1Gordon Wellman2Md. Arfan Ali3Saad Hussain Shah4Yasir Anwar5Magdi Ali Ahmed Mousa6Department of Arid Land Agriculture, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Arid Land Agriculture, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDivision of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi ArabiaDepartment of Arid Land Agriculture, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah 21589, Saudi ArabiaInstitute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar 25120, PakistanDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Arid Land Agriculture, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah 21589, Saudi ArabiaGlobally, drought and salinity stress critically constrain potato (<i>Solanum tuberosum</i> L.) production. Considering the impact of these stresses on crops and increasing food demand, insight into both tolerance and susceptibility is essential. The present study screens two potato cultivars, BARI-401 and Spunta, for their tolerance to simulated salinity and drought by in vitro LiCl and mannitol exposure. Plantlets treated with a range of LiCl (0, 10, 30, and 40 mM) and mannitol (0, 50, 100, 200, and 250 mM) concentrations were biochemically and physiologically characterized to assess their tolerance capacity. Shoot number, shoot length, root number, and root length were affected in both cultivars under higher LiCl and mannitol concentrations, even though Spunta was able to better maintain a higher shoot length under the 40 mM of LiCl and 250 mM of mannitol compared to BARI-401. The total phenol contents (TPC) in both cultivars were increased at the highest treatment concentration and the total flavonoids content (TFC) was decreased in BARI-401 as compared to Spunta. Higher free radical scavenging capacity (FRSC, low IC<sub>50</sub> value) was recorded in Spunta as compared to BARI-401 with increasing treatment concentrations, which supports the high antioxidant capacity of Spunta. An inverse correlation between polyphenol oxidase (PPO) and TPC was noted in both cultivars. Peroxidase dismutase (POD) activity was increased significantly in both cultivars for all treatments, but activity was highest overall in Spunta. These physiological and biochemical analyses of both cultivars suggest that cultivar Spunta is more tolerant to salinity and drought stress. Further open-field experiments are required to confirm these results.https://www.mdpi.com/2223-7747/10/5/924<i>Solanum tuberosum</i> L.micropropagationgrowthabiotic stresstoleranceantioxidants |
| spellingShingle | Farooq Abdul Sattar Bahget Talat Hamooh Gordon Wellman Md. Arfan Ali Saad Hussain Shah Yasir Anwar Magdi Ali Ahmed Mousa Growth and Biochemical Responses of Potato Cultivars under In Vitro Lithium Chloride and Mannitol Simulated Salinity and Drought Stress Plants <i>Solanum tuberosum</i> L. micropropagation growth abiotic stress tolerance antioxidants |
| title | Growth and Biochemical Responses of Potato Cultivars under In Vitro Lithium Chloride and Mannitol Simulated Salinity and Drought Stress |
| title_full | Growth and Biochemical Responses of Potato Cultivars under In Vitro Lithium Chloride and Mannitol Simulated Salinity and Drought Stress |
| title_fullStr | Growth and Biochemical Responses of Potato Cultivars under In Vitro Lithium Chloride and Mannitol Simulated Salinity and Drought Stress |
| title_full_unstemmed | Growth and Biochemical Responses of Potato Cultivars under In Vitro Lithium Chloride and Mannitol Simulated Salinity and Drought Stress |
| title_short | Growth and Biochemical Responses of Potato Cultivars under In Vitro Lithium Chloride and Mannitol Simulated Salinity and Drought Stress |
| title_sort | growth and biochemical responses of potato cultivars under in vitro lithium chloride and mannitol simulated salinity and drought stress |
| topic | <i>Solanum tuberosum</i> L. micropropagation growth abiotic stress tolerance antioxidants |
| url | https://www.mdpi.com/2223-7747/10/5/924 |
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