Multiorgan and Vascular Tropism of SARS-CoV-2
Although the respiratory tract is the main target of SARS-CoV-2, other tissues and organs are permissive to the infection. In this report, we investigated this wide-spectrum tropism by studying the SARS-CoV-2 genetic intra-host variability in multiple tissues. The virological and histological invest...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/3/515 |
| _version_ | 1797441053448470528 |
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| author | Cédric Hartard Ahlam Chaqroun Nicla Settembre Guillaume Gauchotte Benjamin Lefevre Elodie Marchand Charles Mazeaud Duc Trung Nguyen Laurent Martrille Isabelle Koscinski Sergueï Malikov Evelyne Schvoerer |
| author_facet | Cédric Hartard Ahlam Chaqroun Nicla Settembre Guillaume Gauchotte Benjamin Lefevre Elodie Marchand Charles Mazeaud Duc Trung Nguyen Laurent Martrille Isabelle Koscinski Sergueï Malikov Evelyne Schvoerer |
| author_sort | Cédric Hartard |
| collection | DOAJ |
| description | Although the respiratory tract is the main target of SARS-CoV-2, other tissues and organs are permissive to the infection. In this report, we investigated this wide-spectrum tropism by studying the SARS-CoV-2 genetic intra-host variability in multiple tissues. The virological and histological investigation of multiple specimens from a post-mortem COVID-19 patient was performed. SARS-CoV-2 genome was detected in several tissues, including the lower respiratory system, cardio-vascular biopsies, stomach, pancreas, adrenal gland, mediastinal ganglion and testicles. Subgenomic RNA transcripts were also detected, in favor of an active viral replication, especially in testicles. Ultra-deep sequencing allowed us to highlight several SARS-CoV-2 mutations according to tissue distribution. More specifically, mutations of the spike protein, i.e., V341A (18.3%), E654 (44%) and H655R (30.8%), were detected in the inferior vena cava. SARS-CoV-2 variability can contribute to heterogeneous distributions of viral quasispecies, which may affect the COVID-19 pathogeny. |
| first_indexed | 2024-03-09T12:17:22Z |
| format | Article |
| id | doaj.art-f97fd96ad1f943d780b333dff929e538 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:17:22Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f97fd96ad1f943d780b333dff929e5382023-11-30T22:45:39ZengMDPI AGViruses1999-49152022-03-0114351510.3390/v14030515Multiorgan and Vascular Tropism of SARS-CoV-2Cédric Hartard0Ahlam Chaqroun1Nicla Settembre2Guillaume Gauchotte3Benjamin Lefevre4Elodie Marchand5Charles Mazeaud6Duc Trung Nguyen7Laurent Martrille8Isabelle Koscinski9Sergueï Malikov10Evelyne Schvoerer11Laboratory of Virology, Department of Microbiology, CHRU Nancy, Université de Lorraine, F-54000 Nancy, FranceCNRS, LCPME, Université de Lorraine, F-54000 Nancy, FranceDepartment of Vascular & Endovascular Surgery, CHRU Nancy, Université de Lorraine, INSERM 1116, F-54000 Nancy, FranceDepartment of Biopathology, Centre de Ressources Biologiques, Université de Lorraine, F-54000 Nancy, FranceDepartment of Infectious and Tropical Diseases, CHRU Nancy, Université de Lorraine, F-54000 Nancy, FranceDepartment of Legal Medicine, CHU Nancy, Université de Lorraine, F-54000 Nancy, FranceDepartment of Urology, CHRU Nancy, Université de Lorraine, F-54000 Nancy, FranceService ORL, CHRU Nancy, Université de Lorraine, F-54000 Nancy, FranceDepartment of Legal Medicine, CHU Nancy, Université de Lorraine, F-54000 Nancy, FranceLaboratoire de Biologie de la Reproduction/CECOS Lorraine, CHRU Nancy, INSERM U1256 NGERE, F-54000 Nancy, FranceDepartment of Vascular & Endovascular Surgery, CHRU Nancy, Université de Lorraine, INSERM 1116, F-54000 Nancy, FranceLaboratory of Virology, Department of Microbiology, CHRU Nancy, Université de Lorraine, F-54000 Nancy, FranceAlthough the respiratory tract is the main target of SARS-CoV-2, other tissues and organs are permissive to the infection. In this report, we investigated this wide-spectrum tropism by studying the SARS-CoV-2 genetic intra-host variability in multiple tissues. The virological and histological investigation of multiple specimens from a post-mortem COVID-19 patient was performed. SARS-CoV-2 genome was detected in several tissues, including the lower respiratory system, cardio-vascular biopsies, stomach, pancreas, adrenal gland, mediastinal ganglion and testicles. Subgenomic RNA transcripts were also detected, in favor of an active viral replication, especially in testicles. Ultra-deep sequencing allowed us to highlight several SARS-CoV-2 mutations according to tissue distribution. More specifically, mutations of the spike protein, i.e., V341A (18.3%), E654 (44%) and H655R (30.8%), were detected in the inferior vena cava. SARS-CoV-2 variability can contribute to heterogeneous distributions of viral quasispecies, which may affect the COVID-19 pathogeny.https://www.mdpi.com/1999-4915/14/3/515SARS-CoV-2viral quasispeciesultra-deep sequencing |
| spellingShingle | Cédric Hartard Ahlam Chaqroun Nicla Settembre Guillaume Gauchotte Benjamin Lefevre Elodie Marchand Charles Mazeaud Duc Trung Nguyen Laurent Martrille Isabelle Koscinski Sergueï Malikov Evelyne Schvoerer Multiorgan and Vascular Tropism of SARS-CoV-2 Viruses SARS-CoV-2 viral quasispecies ultra-deep sequencing |
| title | Multiorgan and Vascular Tropism of SARS-CoV-2 |
| title_full | Multiorgan and Vascular Tropism of SARS-CoV-2 |
| title_fullStr | Multiorgan and Vascular Tropism of SARS-CoV-2 |
| title_full_unstemmed | Multiorgan and Vascular Tropism of SARS-CoV-2 |
| title_short | Multiorgan and Vascular Tropism of SARS-CoV-2 |
| title_sort | multiorgan and vascular tropism of sars cov 2 |
| topic | SARS-CoV-2 viral quasispecies ultra-deep sequencing |
| url | https://www.mdpi.com/1999-4915/14/3/515 |
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