Molecular characterization of rifabutin-resistance in refractory Helicobacter pylori infection in Taiwan
Objectives: To explore the molecular characteristics of rpoB, encoding β-subunit of DNA-directed RNA polymerase, and unravel the link to rifabutin-resistance in patients with refractory Helicobacter pylori infection. Methods: From January 2018-March 2021, a total of 1590 patients were screened for e...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971223007622 |
| _version_ | 1827583874240086016 |
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| author | Chia-Jung Kuo Ngoc-Niem Bui Jun-Nong Ke Cheng-Yu Lin Wey-Ran Lin Ming-Ling Chang Hui-Yu Wu Mei-Zi Huang Cheng-Hsun Chiu Cheng-Tang Chiu Chih-Ho Lai |
| author_facet | Chia-Jung Kuo Ngoc-Niem Bui Jun-Nong Ke Cheng-Yu Lin Wey-Ran Lin Ming-Ling Chang Hui-Yu Wu Mei-Zi Huang Cheng-Hsun Chiu Cheng-Tang Chiu Chih-Ho Lai |
| author_sort | Chia-Jung Kuo |
| collection | DOAJ |
| description | Objectives: To explore the molecular characteristics of rpoB, encoding β-subunit of DNA-directed RNA polymerase, and unravel the link to rifabutin-resistance in patients with refractory Helicobacter pylori infection. Methods: From January 2018-March 2021, a total of 1590 patients were screened for eligibility to participate in the study. Patients with refractory H. pylori infection were confirmed by using the (13C)-urea breath assay. All enrolled patients underwent esophagogastroduodenoscopy, and biopsies were taken for H. pylori culture and antibacterial susceptibility testing. Sequence analysis of rpoB was conducted for all rifabutin-resistant isolates. Results: In total, 70 patients were diagnosed with refractory H. pylori infection, and 39 isolates were successfully cultured. Amongst, 10 isolates were identified as rifabutin-resistance and nine isolates exhibited at least one amino acid substitution in RpoB. Isolates with a minimal inhibitory concentration >32 mg/l displayed a higher number of mutational changes in RpoB than the others. Additionally, more amino acid substitutions in RpoB correlated with developing a higher minimal inhibitory concentration for H. pylori rifabutin-resistance. Conclusion: Our findings highlight the relationship between rifabutin-resistance in refractory H. pylori infection and specific mutations in RpoB, which will aid the clinical selection of appropriate antibacterial agents with better therapeutic effects. |
| first_indexed | 2024-03-08T23:13:59Z |
| format | Article |
| id | doaj.art-f98411d8054b4e5f8c9c2dcc23907b0f |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-03-08T23:13:59Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-f98411d8054b4e5f8c9c2dcc23907b0f2023-12-15T07:22:47ZengElsevierInternational Journal of Infectious Diseases1201-97122024-01-011382528Molecular characterization of rifabutin-resistance in refractory Helicobacter pylori infection in TaiwanChia-Jung Kuo0Ngoc-Niem Bui1Jun-Nong Ke2Cheng-Yu Lin3Wey-Ran Lin4Ming-Ling Chang5Hui-Yu Wu6Mei-Zi Huang7Cheng-Hsun Chiu8Cheng-Tang Chiu9Chih-Ho Lai10Department of Gastroenterology and Hepatology, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; School of Medicine, College of Medicine, Chang Gung University, Taoyuan, TaiwanGraduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Faculty of Medicine, Can Tho University of Medicine and Pharmacy, Can Tho, Viet NamGraduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Department of Laboratory Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan, TaiwanDepartment of Gastroenterology and Hepatology, Chang Gung Memorial Hospital at Linkou, Taoyuan, TaiwanDepartment of Gastroenterology and Hepatology, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; School of Medicine, College of Medicine, Chang Gung University, Taoyuan, TaiwanDepartment of Gastroenterology and Hepatology, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; School of Medicine, College of Medicine, Chang Gung University, Taoyuan, TaiwanGraduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, TaiwanGraduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, TaiwanSchool of Medicine, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Molecular Infectious Disease Research Center, Department of Pediatrics, Chang Gung Memorial Hospital at Linkou, Taoyuan, TaiwanDepartment of Gastroenterology and Hepatology, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; School of Medicine, College of Medicine, Chang Gung University, Taoyuan, TaiwanGraduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Molecular Infectious Disease Research Center, Department of Pediatrics, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; Department of Medical Research, School of Medicine, China Medical University and Hospital, Taichung, Taiwan; Department of Nursing, Asia University, Taichung, Taiwan; Department of Microbiology and Immunology, Chang Gung University, Taoyuan, Taiwan; Corresponding author.Objectives: To explore the molecular characteristics of rpoB, encoding β-subunit of DNA-directed RNA polymerase, and unravel the link to rifabutin-resistance in patients with refractory Helicobacter pylori infection. Methods: From January 2018-March 2021, a total of 1590 patients were screened for eligibility to participate in the study. Patients with refractory H. pylori infection were confirmed by using the (13C)-urea breath assay. All enrolled patients underwent esophagogastroduodenoscopy, and biopsies were taken for H. pylori culture and antibacterial susceptibility testing. Sequence analysis of rpoB was conducted for all rifabutin-resistant isolates. Results: In total, 70 patients were diagnosed with refractory H. pylori infection, and 39 isolates were successfully cultured. Amongst, 10 isolates were identified as rifabutin-resistance and nine isolates exhibited at least one amino acid substitution in RpoB. Isolates with a minimal inhibitory concentration >32 mg/l displayed a higher number of mutational changes in RpoB than the others. Additionally, more amino acid substitutions in RpoB correlated with developing a higher minimal inhibitory concentration for H. pylori rifabutin-resistance. Conclusion: Our findings highlight the relationship between rifabutin-resistance in refractory H. pylori infection and specific mutations in RpoB, which will aid the clinical selection of appropriate antibacterial agents with better therapeutic effects.http://www.sciencedirect.com/science/article/pii/S1201971223007622Helicobacter pyloriRefractory infectionRifabutin-resistanceRpoBMutation |
| spellingShingle | Chia-Jung Kuo Ngoc-Niem Bui Jun-Nong Ke Cheng-Yu Lin Wey-Ran Lin Ming-Ling Chang Hui-Yu Wu Mei-Zi Huang Cheng-Hsun Chiu Cheng-Tang Chiu Chih-Ho Lai Molecular characterization of rifabutin-resistance in refractory Helicobacter pylori infection in Taiwan International Journal of Infectious Diseases Helicobacter pylori Refractory infection Rifabutin-resistance RpoB Mutation |
| title | Molecular characterization of rifabutin-resistance in refractory Helicobacter pylori infection in Taiwan |
| title_full | Molecular characterization of rifabutin-resistance in refractory Helicobacter pylori infection in Taiwan |
| title_fullStr | Molecular characterization of rifabutin-resistance in refractory Helicobacter pylori infection in Taiwan |
| title_full_unstemmed | Molecular characterization of rifabutin-resistance in refractory Helicobacter pylori infection in Taiwan |
| title_short | Molecular characterization of rifabutin-resistance in refractory Helicobacter pylori infection in Taiwan |
| title_sort | molecular characterization of rifabutin resistance in refractory helicobacter pylori infection in taiwan |
| topic | Helicobacter pylori Refractory infection Rifabutin-resistance RpoB Mutation |
| url | http://www.sciencedirect.com/science/article/pii/S1201971223007622 |
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