Protein kinase C is involved with upstream signaling of methyl farnesoate for photoperiod-dependent sex determination in the water flea Daphnia pulex
Sex determination of Daphnia pulex is decided by environmental conditions. We established a suitable experimental system for this study using D. pulex WTN6 strain, in which the sex of the offspring can be controlled by photoperiod. Long-day conditions induced females and short-day conditions induced...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2017-02-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/6/2/161 |
| _version_ | 1830140746412326912 |
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| author | Kenji Toyota Tomomi Sato Norihisa Tatarazako Taisen Iguchi |
| author_facet | Kenji Toyota Tomomi Sato Norihisa Tatarazako Taisen Iguchi |
| author_sort | Kenji Toyota |
| collection | DOAJ |
| description | Sex determination of Daphnia pulex is decided by environmental conditions. We established a suitable experimental system for this study using D. pulex WTN6 strain, in which the sex of the offspring can be controlled by photoperiod. Long-day conditions induced females and short-day conditions induced males. Using this system, we previously found that methy farnesoate (MF), which is a putative innate juvenile hormone molecule in daphnids, is necessary for male sex determination and that protein kinase C (PKC) is a candidate factor of male sex determiner. In this study, we demonstrated that a PKC inhibitor [bisindolylmaleimide IV (BIM)] application strongly suppressed male offspring induction in the short-day condition. Moreover, co-treatment of BIM with MF revealed that PKC signaling acts upstream of MF signaling for male sex determination. This is the first experimental evidence that PKC is involved in the male sex determination process associated with methyl farnesoate signaling in daphnid species. |
| first_indexed | 2024-12-17T09:08:23Z |
| format | Article |
| id | doaj.art-f98480939f6945ce8e97530b1aafb5c6 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-17T09:08:23Z |
| publishDate | 2017-02-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-f98480939f6945ce8e97530b1aafb5c62022-12-21T21:55:23ZengThe Company of BiologistsBiology Open2046-63902017-02-016216116410.1242/bio.021857021857Protein kinase C is involved with upstream signaling of methyl farnesoate for photoperiod-dependent sex determination in the water flea Daphnia pulexKenji Toyota0Tomomi Sato1Norihisa Tatarazako2Taisen Iguchi3 Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Department of Basic Biology, Faculty of Life Science, SOKENDAI (Graduate University for Advanced Studies), 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan Ecotoxicity Reference Laboratory, Risk Assessment Science Collaboration Office, Center for Health and Environmental Risk Research, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Department of Basic Biology, Faculty of Life Science, SOKENDAI (Graduate University for Advanced Studies), 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan Sex determination of Daphnia pulex is decided by environmental conditions. We established a suitable experimental system for this study using D. pulex WTN6 strain, in which the sex of the offspring can be controlled by photoperiod. Long-day conditions induced females and short-day conditions induced males. Using this system, we previously found that methy farnesoate (MF), which is a putative innate juvenile hormone molecule in daphnids, is necessary for male sex determination and that protein kinase C (PKC) is a candidate factor of male sex determiner. In this study, we demonstrated that a PKC inhibitor [bisindolylmaleimide IV (BIM)] application strongly suppressed male offspring induction in the short-day condition. Moreover, co-treatment of BIM with MF revealed that PKC signaling acts upstream of MF signaling for male sex determination. This is the first experimental evidence that PKC is involved in the male sex determination process associated with methyl farnesoate signaling in daphnid species.http://bio.biologists.org/content/6/2/161Daphnia pulexEnvironmental sex determinationJuvenile hormoneMethyl farnesoatePhotoperiodProtein kinase C |
| spellingShingle | Kenji Toyota Tomomi Sato Norihisa Tatarazako Taisen Iguchi Protein kinase C is involved with upstream signaling of methyl farnesoate for photoperiod-dependent sex determination in the water flea Daphnia pulex Biology Open Daphnia pulex Environmental sex determination Juvenile hormone Methyl farnesoate Photoperiod Protein kinase C |
| title | Protein kinase C is involved with upstream signaling of methyl farnesoate for photoperiod-dependent sex determination in the water flea Daphnia pulex |
| title_full | Protein kinase C is involved with upstream signaling of methyl farnesoate for photoperiod-dependent sex determination in the water flea Daphnia pulex |
| title_fullStr | Protein kinase C is involved with upstream signaling of methyl farnesoate for photoperiod-dependent sex determination in the water flea Daphnia pulex |
| title_full_unstemmed | Protein kinase C is involved with upstream signaling of methyl farnesoate for photoperiod-dependent sex determination in the water flea Daphnia pulex |
| title_short | Protein kinase C is involved with upstream signaling of methyl farnesoate for photoperiod-dependent sex determination in the water flea Daphnia pulex |
| title_sort | protein kinase c is involved with upstream signaling of methyl farnesoate for photoperiod dependent sex determination in the water flea daphnia pulex |
| topic | Daphnia pulex Environmental sex determination Juvenile hormone Methyl farnesoate Photoperiod Protein kinase C |
| url | http://bio.biologists.org/content/6/2/161 |
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