Photobiomodulation augments the effects of mitochondrial transplantation in the treatment of spinal cord injury in rats by facilitating mitochondrial transfer to neurons via Connexin 36
Abstract Mitochondrial transplantation is a promising treatment for spinal cord injury (SCI), but it has the disadvantage of low efficiency of mitochondrial transfer to targeted cells. Here, we demonstrated that Photobiomodulation (PBM) could promote the transfer process, thus augmenting the therape...
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| Format: | Article |
| Language: | English |
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Wiley
2023-05-01
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| Series: | Bioengineering & Translational Medicine |
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| Online Access: | https://doi.org/10.1002/btm2.10473 |
| _version_ | 1797825430354395136 |
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| author | Zhijie Zhu Xin Li Xuankang Wang Xiaoshuang Zuo Yangguang Ma Xue Gao Zhuowen Liang Zhihao Zhang Zhiwen Song Tan Ding Cheng Ju Penghui Li Kun Li Jiawei Zhang Huilin Quan Zhe Wang Xueyu Hu |
| author_facet | Zhijie Zhu Xin Li Xuankang Wang Xiaoshuang Zuo Yangguang Ma Xue Gao Zhuowen Liang Zhihao Zhang Zhiwen Song Tan Ding Cheng Ju Penghui Li Kun Li Jiawei Zhang Huilin Quan Zhe Wang Xueyu Hu |
| author_sort | Zhijie Zhu |
| collection | DOAJ |
| description | Abstract Mitochondrial transplantation is a promising treatment for spinal cord injury (SCI), but it has the disadvantage of low efficiency of mitochondrial transfer to targeted cells. Here, we demonstrated that Photobiomodulation (PBM) could promote the transfer process, thus augmenting the therapeutic effect of mitochondrial transplantation. In vivo experiments, motor function recovery, tissue repair, and neuronal apoptosis were evaluated in different treatment groups. Under the premise of mitochondrial transplantation, the expression of Connex36 (Cx36), the trend of mitochondria transferred to neurons, and its downstream effects, such as ATP production and antioxidant capacity, were evaluated after PBM intervention. In in vitro experiments, dorsal root ganglia (DRG) were cotreated with PBM and 18β‐GA (a Cx36 inhibitor). In vivo experiments showed that PBM combined with mitochondrial transplantation could increase ATP production and reduce oxidative stress and neuronal apoptosis levels, thereby promoting tissue repair and motor function recovery. In vitro experiments further verified that Cx36 mediated the transfer of mitochondria into neurons. PBM could facilitate this progress via Cx36 both in vivo and in vitro. The present study reports a potential method of using PBM to facilitate the transfer of mitochondria to neurons for the treatment of SCI. |
| first_indexed | 2024-03-13T10:52:52Z |
| format | Article |
| id | doaj.art-f98851e091f0443eba4ecefe958ef868 |
| institution | Directory Open Access Journal |
| issn | 2380-6761 |
| language | English |
| last_indexed | 2024-03-13T10:52:52Z |
| publishDate | 2023-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj.art-f98851e091f0443eba4ecefe958ef8682023-05-17T07:33:11ZengWileyBioengineering & Translational Medicine2380-67612023-05-0183n/an/a10.1002/btm2.10473Photobiomodulation augments the effects of mitochondrial transplantation in the treatment of spinal cord injury in rats by facilitating mitochondrial transfer to neurons via Connexin 36Zhijie Zhu0Xin Li1Xuankang Wang2Xiaoshuang Zuo3Yangguang Ma4Xue Gao5Zhuowen Liang6Zhihao Zhang7Zhiwen Song8Tan Ding9Cheng Ju10Penghui Li11Kun Li12Jiawei Zhang13Huilin Quan14Zhe Wang15Xueyu Hu16Department of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaDepartment of Orthopedics Xijing Hospital, Fourth Military Medical University Shaanxi ChinaAbstract Mitochondrial transplantation is a promising treatment for spinal cord injury (SCI), but it has the disadvantage of low efficiency of mitochondrial transfer to targeted cells. Here, we demonstrated that Photobiomodulation (PBM) could promote the transfer process, thus augmenting the therapeutic effect of mitochondrial transplantation. In vivo experiments, motor function recovery, tissue repair, and neuronal apoptosis were evaluated in different treatment groups. Under the premise of mitochondrial transplantation, the expression of Connex36 (Cx36), the trend of mitochondria transferred to neurons, and its downstream effects, such as ATP production and antioxidant capacity, were evaluated after PBM intervention. In in vitro experiments, dorsal root ganglia (DRG) were cotreated with PBM and 18β‐GA (a Cx36 inhibitor). In vivo experiments showed that PBM combined with mitochondrial transplantation could increase ATP production and reduce oxidative stress and neuronal apoptosis levels, thereby promoting tissue repair and motor function recovery. In vitro experiments further verified that Cx36 mediated the transfer of mitochondria into neurons. PBM could facilitate this progress via Cx36 both in vivo and in vitro. The present study reports a potential method of using PBM to facilitate the transfer of mitochondria to neurons for the treatment of SCI.https://doi.org/10.1002/btm2.10473connexinmitochondrial transplantationneuronphotobiomodulationspinal cord injury |
| spellingShingle | Zhijie Zhu Xin Li Xuankang Wang Xiaoshuang Zuo Yangguang Ma Xue Gao Zhuowen Liang Zhihao Zhang Zhiwen Song Tan Ding Cheng Ju Penghui Li Kun Li Jiawei Zhang Huilin Quan Zhe Wang Xueyu Hu Photobiomodulation augments the effects of mitochondrial transplantation in the treatment of spinal cord injury in rats by facilitating mitochondrial transfer to neurons via Connexin 36 Bioengineering & Translational Medicine connexin mitochondrial transplantation neuron photobiomodulation spinal cord injury |
| title | Photobiomodulation augments the effects of mitochondrial transplantation in the treatment of spinal cord injury in rats by facilitating mitochondrial transfer to neurons via Connexin 36 |
| title_full | Photobiomodulation augments the effects of mitochondrial transplantation in the treatment of spinal cord injury in rats by facilitating mitochondrial transfer to neurons via Connexin 36 |
| title_fullStr | Photobiomodulation augments the effects of mitochondrial transplantation in the treatment of spinal cord injury in rats by facilitating mitochondrial transfer to neurons via Connexin 36 |
| title_full_unstemmed | Photobiomodulation augments the effects of mitochondrial transplantation in the treatment of spinal cord injury in rats by facilitating mitochondrial transfer to neurons via Connexin 36 |
| title_short | Photobiomodulation augments the effects of mitochondrial transplantation in the treatment of spinal cord injury in rats by facilitating mitochondrial transfer to neurons via Connexin 36 |
| title_sort | photobiomodulation augments the effects of mitochondrial transplantation in the treatment of spinal cord injury in rats by facilitating mitochondrial transfer to neurons via connexin 36 |
| topic | connexin mitochondrial transplantation neuron photobiomodulation spinal cord injury |
| url | https://doi.org/10.1002/btm2.10473 |
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