Overexpression of phosphoserine aminotransferase PSAT1 stimulates cell growth and increases chemoresistance of colon cancer cells
<p>Abstract</p> <p>Background</p> <p>Colorectal cancer (CRC) is one of the most common causes of cancer death throughout the world. In this work our aim was to study the role of the phosphoserine aminotransferase PSAT1 in colorectal cancer development.</p> <p&g...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-01-01
|
| Series: | Molecular Cancer |
| Online Access: | http://www.molecular-cancer.com/content/7/1/14 |
| _version_ | 1819042327864279040 |
|---|---|
| author | Conseiller Emmanuel Fraslon Caroline Robert Bruno Bec Nicole Denis Vincent Bascoul-Mollevi Caroline Copois Virginie Vié Nadia Molina Franck Larroque Christian Martineau Pierre Del Rio Maguy Gongora Céline |
| author_facet | Conseiller Emmanuel Fraslon Caroline Robert Bruno Bec Nicole Denis Vincent Bascoul-Mollevi Caroline Copois Virginie Vié Nadia Molina Franck Larroque Christian Martineau Pierre Del Rio Maguy Gongora Céline |
| author_sort | Conseiller Emmanuel |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Colorectal cancer (CRC) is one of the most common causes of cancer death throughout the world. In this work our aim was to study the role of the phosphoserine aminotransferase PSAT1 in colorectal cancer development.</p> <p>Results</p> <p>We first observed that PSAT1 is overexpressed in colon tumors. In addition, we showed that after drug treatment, PSAT1 expression level in hepatic metastases increased in non responder and decreased in responder patients.</p> <p>In experiments using human cell lines, we showed that ectopic PSAT1 overexpression in colon carcinoma SW480 cell line resulted in an increase in its growth rate and survival. In addition, SW480-PSAT1 cells presented a higher tumorigenic potential than SW480 control cells in xenografted mice. Moreover, the SW480-PSAT1 cell line was more resistant to oxaliplatin treatment than the non-transfected SW480 cell line. This resistance resulted from a decrease in the apoptotic response and in the mitotic catastrophes induced by the drug treatment.</p> <p>Conclusion</p> <p>These results show that an enzyme playing a role in the L-serine biosynthesis could be implicated in colon cancer progression and chemoresistance and indicate that PSAT1 represents a new interesting target for CRC therapy.</p> |
| first_indexed | 2024-12-21T09:39:08Z |
| format | Article |
| id | doaj.art-f98e3a88f2844e829df68d72df895f82 |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-12-21T09:39:08Z |
| publishDate | 2008-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-f98e3a88f2844e829df68d72df895f822022-12-21T19:08:32ZengBMCMolecular Cancer1476-45982008-01-01711410.1186/1476-4598-7-14Overexpression of phosphoserine aminotransferase PSAT1 stimulates cell growth and increases chemoresistance of colon cancer cellsConseiller EmmanuelFraslon CarolineRobert BrunoBec NicoleDenis VincentBascoul-Mollevi CarolineCopois VirginieVié NadiaMolina FranckLarroque ChristianMartineau PierreDel Rio MaguyGongora Céline<p>Abstract</p> <p>Background</p> <p>Colorectal cancer (CRC) is one of the most common causes of cancer death throughout the world. In this work our aim was to study the role of the phosphoserine aminotransferase PSAT1 in colorectal cancer development.</p> <p>Results</p> <p>We first observed that PSAT1 is overexpressed in colon tumors. In addition, we showed that after drug treatment, PSAT1 expression level in hepatic metastases increased in non responder and decreased in responder patients.</p> <p>In experiments using human cell lines, we showed that ectopic PSAT1 overexpression in colon carcinoma SW480 cell line resulted in an increase in its growth rate and survival. In addition, SW480-PSAT1 cells presented a higher tumorigenic potential than SW480 control cells in xenografted mice. Moreover, the SW480-PSAT1 cell line was more resistant to oxaliplatin treatment than the non-transfected SW480 cell line. This resistance resulted from a decrease in the apoptotic response and in the mitotic catastrophes induced by the drug treatment.</p> <p>Conclusion</p> <p>These results show that an enzyme playing a role in the L-serine biosynthesis could be implicated in colon cancer progression and chemoresistance and indicate that PSAT1 represents a new interesting target for CRC therapy.</p>http://www.molecular-cancer.com/content/7/1/14 |
| spellingShingle | Conseiller Emmanuel Fraslon Caroline Robert Bruno Bec Nicole Denis Vincent Bascoul-Mollevi Caroline Copois Virginie Vié Nadia Molina Franck Larroque Christian Martineau Pierre Del Rio Maguy Gongora Céline Overexpression of phosphoserine aminotransferase PSAT1 stimulates cell growth and increases chemoresistance of colon cancer cells Molecular Cancer |
| title | Overexpression of phosphoserine aminotransferase PSAT1 stimulates cell growth and increases chemoresistance of colon cancer cells |
| title_full | Overexpression of phosphoserine aminotransferase PSAT1 stimulates cell growth and increases chemoresistance of colon cancer cells |
| title_fullStr | Overexpression of phosphoserine aminotransferase PSAT1 stimulates cell growth and increases chemoresistance of colon cancer cells |
| title_full_unstemmed | Overexpression of phosphoserine aminotransferase PSAT1 stimulates cell growth and increases chemoresistance of colon cancer cells |
| title_short | Overexpression of phosphoserine aminotransferase PSAT1 stimulates cell growth and increases chemoresistance of colon cancer cells |
| title_sort | overexpression of phosphoserine aminotransferase psat1 stimulates cell growth and increases chemoresistance of colon cancer cells |
| url | http://www.molecular-cancer.com/content/7/1/14 |
| work_keys_str_mv | AT conseilleremmanuel overexpressionofphosphoserineaminotransferasepsat1stimulatescellgrowthandincreaseschemoresistanceofcoloncancercells AT frasloncaroline overexpressionofphosphoserineaminotransferasepsat1stimulatescellgrowthandincreaseschemoresistanceofcoloncancercells AT robertbruno overexpressionofphosphoserineaminotransferasepsat1stimulatescellgrowthandincreaseschemoresistanceofcoloncancercells AT becnicole overexpressionofphosphoserineaminotransferasepsat1stimulatescellgrowthandincreaseschemoresistanceofcoloncancercells AT denisvincent overexpressionofphosphoserineaminotransferasepsat1stimulatescellgrowthandincreaseschemoresistanceofcoloncancercells AT bascoulmollevicaroline overexpressionofphosphoserineaminotransferasepsat1stimulatescellgrowthandincreaseschemoresistanceofcoloncancercells AT copoisvirginie overexpressionofphosphoserineaminotransferasepsat1stimulatescellgrowthandincreaseschemoresistanceofcoloncancercells AT vienadia overexpressionofphosphoserineaminotransferasepsat1stimulatescellgrowthandincreaseschemoresistanceofcoloncancercells AT molinafranck overexpressionofphosphoserineaminotransferasepsat1stimulatescellgrowthandincreaseschemoresistanceofcoloncancercells AT larroquechristian overexpressionofphosphoserineaminotransferasepsat1stimulatescellgrowthandincreaseschemoresistanceofcoloncancercells AT martineaupierre overexpressionofphosphoserineaminotransferasepsat1stimulatescellgrowthandincreaseschemoresistanceofcoloncancercells AT delriomaguy overexpressionofphosphoserineaminotransferasepsat1stimulatescellgrowthandincreaseschemoresistanceofcoloncancercells AT gongoraceline overexpressionofphosphoserineaminotransferasepsat1stimulatescellgrowthandincreaseschemoresistanceofcoloncancercells |