Spoligotype‐based population structure of Mycobacterium tuberculosis in the Jimma Zone, southwest Ethiopia
Abstract Background To understand the population dynamics and propose more effective preventive strategies, defining the population structure of the circulating Mycobacterium tuberculosis strains is important. Methods A total of 177 M. tuberculosis complex isolates from pulmonary tuberculosis (TB) c...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-06-01
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| Series: | MicrobiologyOpen |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mbo3.744 |
| _version_ | 1818337543107641344 |
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| author | Gemeda Abebe Ketema Abdissa Kedir Abdella Mulualem Tadesse Adane Worku Gobena Ameni |
| author_facet | Gemeda Abebe Ketema Abdissa Kedir Abdella Mulualem Tadesse Adane Worku Gobena Ameni |
| author_sort | Gemeda Abebe |
| collection | DOAJ |
| description | Abstract Background To understand the population dynamics and propose more effective preventive strategies, defining the population structure of the circulating Mycobacterium tuberculosis strains is important. Methods A total of 177 M. tuberculosis complex isolates from pulmonary tuberculosis (TB) cases in southwest Ethiopia were genotyped by spoligotyping. Of the strains included in this study, 126 were pan‐susceptible strains while the remaining 51 isolates were resistant to one or more first‐line anti‐TB drugs. The genotyping results were compared to the international spoligotyping (SITVIT) database of the Pasteur Institute of Guadeloupe and the newly revised publicly available international multi‐marker database (SITVITWEB/SPOLDB4). An online tool Run TB‐Lineage was also used to predict the major lineages using a conformal Bayesian network analysis. Results The spoligotyping of the 177 isolates resulted in 69 different spoligotype patterns of which 127 (71.8%) were clustered into 19 spoligoclusters (with clustering rate of 61.02%). Each cluster contains 2–29 isolates. Of the isolates with corresponding SIT in SITVIT/SDB4, the predominant strains identified were SIT 37 of the T3 subfamily with 29 isolates followed by SIT 53 of the T1 subfamily with 20 isolates. SIT 777 of the H4 subfamily and SIT 25 of the CAS1_DELHI subfamily each consisting of six isolates were identified. Eighty spoligotype patterns were orphan as they were not recorded in the SITVIT2/SPDB4 database. Further classification of the isolates on the basis of major lineages showed that 82.5% and 14.1% of the isolates belonged to Euro‐American and East African Indian lineages, respectively, while 2.8% of the isolates belonged to Mycobacterium africanum and 0.6% to Indo‐Oceanic. Conclusion The ill‐defined T and H clades were predominant around Jimma. The substantial number of orphans recorded in the study area warrants for additional studies with genotyping methods with better resolution and covering whole areas of southwest Ethiopia. |
| first_indexed | 2024-12-13T14:56:53Z |
| format | Article |
| id | doaj.art-f990a67354f642c99e3fdfc859475757 |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-12-13T14:56:53Z |
| publishDate | 2019-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-f990a67354f642c99e3fdfc8594757572022-12-21T23:41:13ZengWileyMicrobiologyOpen2045-88272019-06-0186n/an/a10.1002/mbo3.744Spoligotype‐based population structure of Mycobacterium tuberculosis in the Jimma Zone, southwest EthiopiaGemeda Abebe0Ketema Abdissa1Kedir Abdella2Mulualem Tadesse3Adane Worku4Gobena Ameni5Mycobacteriology Research Center Jimma University Jimma EthiopiaMycobacteriology Research Center Jimma University Jimma EthiopiaMycobacteriology Research Center Jimma University Jimma EthiopiaMycobacteriology Research Center Jimma University Jimma EthiopiaAklilu Lemma Institute of Pathobiology Addis Ababa University Addis Ababa EthiopiaAklilu Lemma Institute of Pathobiology Addis Ababa University Addis Ababa EthiopiaAbstract Background To understand the population dynamics and propose more effective preventive strategies, defining the population structure of the circulating Mycobacterium tuberculosis strains is important. Methods A total of 177 M. tuberculosis complex isolates from pulmonary tuberculosis (TB) cases in southwest Ethiopia were genotyped by spoligotyping. Of the strains included in this study, 126 were pan‐susceptible strains while the remaining 51 isolates were resistant to one or more first‐line anti‐TB drugs. The genotyping results were compared to the international spoligotyping (SITVIT) database of the Pasteur Institute of Guadeloupe and the newly revised publicly available international multi‐marker database (SITVITWEB/SPOLDB4). An online tool Run TB‐Lineage was also used to predict the major lineages using a conformal Bayesian network analysis. Results The spoligotyping of the 177 isolates resulted in 69 different spoligotype patterns of which 127 (71.8%) were clustered into 19 spoligoclusters (with clustering rate of 61.02%). Each cluster contains 2–29 isolates. Of the isolates with corresponding SIT in SITVIT/SDB4, the predominant strains identified were SIT 37 of the T3 subfamily with 29 isolates followed by SIT 53 of the T1 subfamily with 20 isolates. SIT 777 of the H4 subfamily and SIT 25 of the CAS1_DELHI subfamily each consisting of six isolates were identified. Eighty spoligotype patterns were orphan as they were not recorded in the SITVIT2/SPDB4 database. Further classification of the isolates on the basis of major lineages showed that 82.5% and 14.1% of the isolates belonged to Euro‐American and East African Indian lineages, respectively, while 2.8% of the isolates belonged to Mycobacterium africanum and 0.6% to Indo‐Oceanic. Conclusion The ill‐defined T and H clades were predominant around Jimma. The substantial number of orphans recorded in the study area warrants for additional studies with genotyping methods with better resolution and covering whole areas of southwest Ethiopia.https://doi.org/10.1002/mbo3.744EthiopiaJimmaM. tuberculosispopulations structure |
| spellingShingle | Gemeda Abebe Ketema Abdissa Kedir Abdella Mulualem Tadesse Adane Worku Gobena Ameni Spoligotype‐based population structure of Mycobacterium tuberculosis in the Jimma Zone, southwest Ethiopia MicrobiologyOpen Ethiopia Jimma M. tuberculosis populations structure |
| title | Spoligotype‐based population structure of Mycobacterium tuberculosis in the Jimma Zone, southwest Ethiopia |
| title_full | Spoligotype‐based population structure of Mycobacterium tuberculosis in the Jimma Zone, southwest Ethiopia |
| title_fullStr | Spoligotype‐based population structure of Mycobacterium tuberculosis in the Jimma Zone, southwest Ethiopia |
| title_full_unstemmed | Spoligotype‐based population structure of Mycobacterium tuberculosis in the Jimma Zone, southwest Ethiopia |
| title_short | Spoligotype‐based population structure of Mycobacterium tuberculosis in the Jimma Zone, southwest Ethiopia |
| title_sort | spoligotype based population structure of mycobacterium tuberculosis in the jimma zone southwest ethiopia |
| topic | Ethiopia Jimma M. tuberculosis populations structure |
| url | https://doi.org/10.1002/mbo3.744 |
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