Actions of FGF2, LIF, and IGF1 on bovine embryo survival and conceptus elongation following slow-rate freezing
Culture environment during in vitro embryo production can affect embryo phenotype and pregnancy outcomes, making culture modifications a logical approach for improving embryo competence. Previously, the addition of the growth factors FGF2, LIF, and IGF1, termed FLI, to the culture medium improved bo...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Animal Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fanim.2022.1040064/full |
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| author | Katy S. Stoecklein Alvaro Garcia-Guerra Benjamin J. Duran Randall S. Prather M. Sofia Ortega M. Sofia Ortega |
| author_facet | Katy S. Stoecklein Alvaro Garcia-Guerra Benjamin J. Duran Randall S. Prather M. Sofia Ortega M. Sofia Ortega |
| author_sort | Katy S. Stoecklein |
| collection | DOAJ |
| description | Culture environment during in vitro embryo production can affect embryo phenotype and pregnancy outcomes, making culture modifications a logical approach for improving embryo competence. Previously, the addition of the growth factors FGF2, LIF, and IGF1, termed FLI, to the culture medium improved bovine embryo development, and re-expansion following cryopreservation. The objective of this study was to investigate the survival of cryopreserved FLI treated embryos at day 15 of pregnancy and evaluate conceptus transcriptomes. Embryos were produced using in vitro fertilization of abattoir-derived oocytes, cultured to the blastocyst stage in the presence or absence of FLI (+/- FLI), and cryopreserved by slow-rate freezing. Thawed embryos were transferred into non-lactating recipient beef cows and eight days later conceptuses were recovered and analyzed. For a subset of conceptuses whole transcriptome analysis was performed by using the NovaSeq 6000. There was no detectable difference in conceptus recovery or average conceptus length between the two groups. There were 32 differentially expressed transcripts, 23 up-regulated and nine down-regulated in the +FLI group compared to -FLI. Genes were involved in interferon signaling, prostaglandin synthesis, and placental development. This study reveals that embryos cultured with or without FLI and cryopreserved by slow-rate freezing have similar developmental competence up to day 15 of development. Nevertheless, differences in gene expression exhibit an effect of FLI on conceptus signaling during elongation. |
| first_indexed | 2024-04-11T14:32:36Z |
| format | Article |
| id | doaj.art-f991d470adc049bda8ea152e35f7311f |
| institution | Directory Open Access Journal |
| issn | 2673-6225 |
| language | English |
| last_indexed | 2024-04-11T14:32:36Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Animal Science |
| spelling | doaj.art-f991d470adc049bda8ea152e35f7311f2022-12-22T04:18:33ZengFrontiers Media S.A.Frontiers in Animal Science2673-62252022-11-01310.3389/fanim.2022.10400641040064Actions of FGF2, LIF, and IGF1 on bovine embryo survival and conceptus elongation following slow-rate freezingKaty S. Stoecklein0Alvaro Garcia-Guerra1Benjamin J. Duran2Randall S. Prather3M. Sofia Ortega4M. Sofia Ortega5Division of Animal Sciences, University of Missouri, Columbia, MO, United StatesDepartment of Animal Sciences, The Ohio State University, Columbus, OH, United StatesDepartment of Animal Sciences, The Ohio State University, Columbus, OH, United StatesDivision of Animal Sciences, University of Missouri, Columbia, MO, United StatesDivision of Animal Sciences, University of Missouri, Columbia, MO, United StatesDepartment of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI, United StatesCulture environment during in vitro embryo production can affect embryo phenotype and pregnancy outcomes, making culture modifications a logical approach for improving embryo competence. Previously, the addition of the growth factors FGF2, LIF, and IGF1, termed FLI, to the culture medium improved bovine embryo development, and re-expansion following cryopreservation. The objective of this study was to investigate the survival of cryopreserved FLI treated embryos at day 15 of pregnancy and evaluate conceptus transcriptomes. Embryos were produced using in vitro fertilization of abattoir-derived oocytes, cultured to the blastocyst stage in the presence or absence of FLI (+/- FLI), and cryopreserved by slow-rate freezing. Thawed embryos were transferred into non-lactating recipient beef cows and eight days later conceptuses were recovered and analyzed. For a subset of conceptuses whole transcriptome analysis was performed by using the NovaSeq 6000. There was no detectable difference in conceptus recovery or average conceptus length between the two groups. There were 32 differentially expressed transcripts, 23 up-regulated and nine down-regulated in the +FLI group compared to -FLI. Genes were involved in interferon signaling, prostaglandin synthesis, and placental development. This study reveals that embryos cultured with or without FLI and cryopreserved by slow-rate freezing have similar developmental competence up to day 15 of development. Nevertheless, differences in gene expression exhibit an effect of FLI on conceptus signaling during elongation.https://www.frontiersin.org/articles/10.3389/fanim.2022.1040064/fullin vitro fertilizationbovineconceptus elongationFGF2LIFIGF1 |
| spellingShingle | Katy S. Stoecklein Alvaro Garcia-Guerra Benjamin J. Duran Randall S. Prather M. Sofia Ortega M. Sofia Ortega Actions of FGF2, LIF, and IGF1 on bovine embryo survival and conceptus elongation following slow-rate freezing Frontiers in Animal Science in vitro fertilization bovine conceptus elongation FGF2 LIF IGF1 |
| title | Actions of FGF2, LIF, and IGF1 on bovine embryo survival and conceptus elongation following slow-rate freezing |
| title_full | Actions of FGF2, LIF, and IGF1 on bovine embryo survival and conceptus elongation following slow-rate freezing |
| title_fullStr | Actions of FGF2, LIF, and IGF1 on bovine embryo survival and conceptus elongation following slow-rate freezing |
| title_full_unstemmed | Actions of FGF2, LIF, and IGF1 on bovine embryo survival and conceptus elongation following slow-rate freezing |
| title_short | Actions of FGF2, LIF, and IGF1 on bovine embryo survival and conceptus elongation following slow-rate freezing |
| title_sort | actions of fgf2 lif and igf1 on bovine embryo survival and conceptus elongation following slow rate freezing |
| topic | in vitro fertilization bovine conceptus elongation FGF2 LIF IGF1 |
| url | https://www.frontiersin.org/articles/10.3389/fanim.2022.1040064/full |
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