Escherichia coli‐associated follicular cystitis in dogs: Clinical and pathologic characterization
Abstract Background Follicular cystitis is an uncommon inflammatory change in the urinary bladder wall characterized by the formation of tertiary lymphoid structures (TLSs) in the submucosa. Objectives To characterize clinical and pathologic features of follicular cystitis in dogs and to explore in...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-05-01
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| Series: | Journal of Veterinary Internal Medicine |
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| Online Access: | https://doi.org/10.1111/jvim.16719 |
| _version_ | 1797816151319773184 |
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| author | Sanna J. Viitanen Laura Tuomisto Nina Salonen Katariina Eskola Kristel Kegler |
| author_facet | Sanna J. Viitanen Laura Tuomisto Nina Salonen Katariina Eskola Kristel Kegler |
| author_sort | Sanna J. Viitanen |
| collection | DOAJ |
| description | Abstract Background Follicular cystitis is an uncommon inflammatory change in the urinary bladder wall characterized by the formation of tertiary lymphoid structures (TLSs) in the submucosa. Objectives To characterize clinical and pathologic features of follicular cystitis in dogs and to explore in situ distribution and possible role of Escherichia coli as an associated cause. Animals Eight dogs diagnosed with follicular cystitis and 2 control dogs. Methods Retrospective descriptive study. Dogs diagnosed with follicular cystitis (macroscopic follicular lesions in the urinary bladder mucosa and histopathologic detection of TLSs in bladder wall biopsies) were identified from medical records. Paraffin embedded bladder wall biopsies were subject to in situ hybridization for E. coli 16SrRNA identification. Results Follicular cystitis was diagnosed in large breed (median weight 24.9 kg, interquartile range [IQR] 18.8‐35.4 kg) female dogs with a history of chronic recurrent urinary tract infections (UTIs; median duration of clinical signs 7 months, IQR 3‐17 months; median number of previous UTIs 5, IQR 4‐6). Positive E. coli 16SrRNA signal was detected within developing, immature and mature TLSs in 7/8 dogs, through submucosal stroma in 8/8 dogs and within the urothelium in 3/8 dogs. Conclusions and Clinical Importance Chronic inflammation associated with an intramural E. coli infection in the urinary bladder wall represents a possible triggering factor for the development of follicular cystitis. |
| first_indexed | 2024-03-13T08:33:26Z |
| format | Article |
| id | doaj.art-f992c45277a94dbf8334a8fe6a43e1c9 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-03-13T08:33:26Z |
| publishDate | 2023-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-f992c45277a94dbf8334a8fe6a43e1c92023-05-31T01:33:58ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762023-05-013731059106610.1111/jvim.16719Escherichia coli‐associated follicular cystitis in dogs: Clinical and pathologic characterizationSanna J. Viitanen0Laura Tuomisto1Nina Salonen2Katariina Eskola3Kristel Kegler4Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine University of Helsinki Helsinki FinlandDepartment of Veterinary Biosciences, Pathology and Parasitology Unit, Faculty of Veterinary Medicine University of Helsinki Helsinki FinlandDepartment of Equine and Small Animal Medicine, Faculty of Veterinary Medicine University of Helsinki Helsinki FinlandDepartment of Equine and Small Animal Medicine, Faculty of Veterinary Medicine University of Helsinki Helsinki FinlandDepartment of Veterinary Biosciences, Pathology and Parasitology Unit, Faculty of Veterinary Medicine University of Helsinki Helsinki FinlandAbstract Background Follicular cystitis is an uncommon inflammatory change in the urinary bladder wall characterized by the formation of tertiary lymphoid structures (TLSs) in the submucosa. Objectives To characterize clinical and pathologic features of follicular cystitis in dogs and to explore in situ distribution and possible role of Escherichia coli as an associated cause. Animals Eight dogs diagnosed with follicular cystitis and 2 control dogs. Methods Retrospective descriptive study. Dogs diagnosed with follicular cystitis (macroscopic follicular lesions in the urinary bladder mucosa and histopathologic detection of TLSs in bladder wall biopsies) were identified from medical records. Paraffin embedded bladder wall biopsies were subject to in situ hybridization for E. coli 16SrRNA identification. Results Follicular cystitis was diagnosed in large breed (median weight 24.9 kg, interquartile range [IQR] 18.8‐35.4 kg) female dogs with a history of chronic recurrent urinary tract infections (UTIs; median duration of clinical signs 7 months, IQR 3‐17 months; median number of previous UTIs 5, IQR 4‐6). Positive E. coli 16SrRNA signal was detected within developing, immature and mature TLSs in 7/8 dogs, through submucosal stroma in 8/8 dogs and within the urothelium in 3/8 dogs. Conclusions and Clinical Importance Chronic inflammation associated with an intramural E. coli infection in the urinary bladder wall represents a possible triggering factor for the development of follicular cystitis.https://doi.org/10.1111/jvim.16719caninehistopathologyin situ hybridizationurinary |
| spellingShingle | Sanna J. Viitanen Laura Tuomisto Nina Salonen Katariina Eskola Kristel Kegler Escherichia coli‐associated follicular cystitis in dogs: Clinical and pathologic characterization Journal of Veterinary Internal Medicine canine histopathology in situ hybridization urinary |
| title | Escherichia coli‐associated follicular cystitis in dogs: Clinical and pathologic characterization |
| title_full | Escherichia coli‐associated follicular cystitis in dogs: Clinical and pathologic characterization |
| title_fullStr | Escherichia coli‐associated follicular cystitis in dogs: Clinical and pathologic characterization |
| title_full_unstemmed | Escherichia coli‐associated follicular cystitis in dogs: Clinical and pathologic characterization |
| title_short | Escherichia coli‐associated follicular cystitis in dogs: Clinical and pathologic characterization |
| title_sort | escherichia coli associated follicular cystitis in dogs clinical and pathologic characterization |
| topic | canine histopathology in situ hybridization urinary |
| url | https://doi.org/10.1111/jvim.16719 |
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